Influence of different kinds of ionizing radiation and cultivation time duration on genetic variability level of soybean somaclones
Автор: Fisenko P.P., Babikova A.V., Yefremova O.S.
Статья в выпуске: 1 (142-143), 2010 года.
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Genetic diversity and relations between plants of Hodson variety of mutant populations developed after seed treat-ment by different kinds of radiation (Hodson-L and Hodson-γ) and their somaclones were studied by means of inter-micro satellite sequence repeat (ISSR) markers. Usage of 12 ISSR-primers allowed to observe variability of 183 ISSR-fragments, 89.61 % of them were polymorphic. Mutant populations Hodson-L and Hodson-γ are characterized by richer genetic variabili-ty than populations of Hodson variety. Genetic variability level of somaclones developed from cotyledon's nodes of the mu-tant population plants decreases and becomes close to the level of Hodson variety variability. However, among these somaclones there were observed regenerant lines, which are characterized by the higher content of protein, histidin, linoleic and oleic acids, and also less damaged by Cercospora sojina and Septoria glycines, than plants of their parental forms. Culti-vation time of initial explants on medium does not influence on the level of the somaclones' genetic variability.
Короткий адрес: https://sciup.org/142150939
IDR: 142150939