Intrapopulation variability of broomrape affecting sunflower in Russia which can be discovered by microsatellite loci

Information about a structure of broomrape populations on sunflower and dynamics of it variability helps breeders to find out a strategy controlling this parasite plant. The purpose of the current research was to study a discriminating potential of a system of microsatellite markers used for determination of in-trapopulation variability of О. cumana infesting sunflower crops in Russia, to analyze a genetic diversity, structure, similarity and discrepancy degree of modern broomrape populations, and to compare populations from Russia and populations from Spain and Ro-mania. For work we used the matured seeds of O. cumana from eight populations collected in 2003-2014 in fields of Krasnodar, Stavropol and Rostov regions, as well as in provinces Cordoba, Spain and Kalarashi, Romania. During research there was determined a race structure of broomrape populations, was done PCR-analysis with ten microsatellite loci and genetic diversity was analyzed using a program GenAlЕx 6.5. A discriminating potential of used marker system is ascertained to be quite informative for determination of intrapopulation variability of broomrape, growing on the territory of Russia. The general traits of genetic diversity showed to have a low level of intrapopulation polymorphism both in the Russian and foreign broomrape populations. An obvious relation between race structure and genetic diversity of populations was not observed. Among the Russian populations, kagalnitskaya and krylovskaya appeared to be the most genetic variable, and privol-nenskaya one is least variable. The Russian populations were differed with more intrapopulation variability compared to Spanish and Romanian. Genetic distances between the Russian populations were generally less than the same one with Spanish and Romanian. A level of genetic differentiation between studied populations calculated by AMOVA appeared to be rather high. The most of revealed genetic diversity (57%) accrued to interpopulation part, intrapopu-lation variability was 38%, dispersion between individuals within each population is 6%. Differences between population calculated by Right’s F-statistics (Fst = 0,565) also certify their genetic differentiation.