Selection of working parameters for the production of egg protein peptides

The production of functional food ingredients, including protein hydrolysates, is one of the innovative areas in the food industry. Enzymatic hydrolysis of proteins is a widely used method for modifying their functional and technological properties and biological activity. The aim of this study was to obtain the hydrolysate of highly hydrolyzed egg albumin based on mathematical experimental planning methods. A two-stage process of enzymatic conversion of egg albumin was carried out. A series of two-factor studies was carried out in the direction of the maximum hydrolysis degree, which was experimentally obtained. Two indicators of enzymatic modification - hydrolysis time and enzyme-substrate ratio (FSS) were studied. The results were optimized using the response surface methodology in the Mathcad 15 professional program. A hydrolysate was obtained with a degree of hydrolysis of 82.56. The parameters of this process were determined: first hydrolysis by pepsin of 5 hours at FSS 1:20, and then - trypsin of 3 hours at FSS 1:19. Further research will focus on the biological activity and functional and technological properties of hydrolysate, as well as the determination of its residual antigenicity. This is the most important characteristic of protein hydrolysates for producing hypoallergenic foods.