Analysis of antibiotic resistance of intestinal microflora bacteria

Currently, there is little information on the levels and changes in pathogenic bacteria of cattle feces and their antibiotic resistance on dairy farms. Monitoring of antibiotic resistance of bacteria isolated from livestock farm waste is necessary. The purpose of the study is to investigate the composition of fecal microflora and analyze its antibiotic resistance. Using routine bacteriological methods, as well as mass spectrometry, the species composition of fecal microflora of cattle was determined, and the disk diffusion method was used to analyze its antibiotic resistance. In the course of the work, data were obtained on the quantitative and qualitative composition of the intestinal microbiome of cattle and the specificity of antibiotic resistance to such drugs as ampicillin in two dosages (2 μg and 10 μg), amoxicillin + clavulanic acid 20/10 μg, benzylpenicillin 1 ME, tetracycline 30 μg, erythromycin 15 μg was revealed. These groups of antibiotics are most often used in animal husbandry. The study will help to determine the antibiotics to which the bacteria of the intestinal microflora of cattle are resistant, since, due to their excessive use, resistance to them appears not only in pathogenic bacteria, but also in the so-called opportunistic microorganisms, of which there are many more, and normally they are not dangerous, but with weakened immunity they can cause various diseases. High resistance of intestinal microflora bacteria to the most popular drugs used in animal husbandry, in particular on dairy farms for the treatment of cattle, has been revealed. Antimicrobial drugs are a tool used on livestock farms to ensure animal health, and this tool should be used rationally.