Biological effects of mitochondrial therapy: preventing development of myocardial infarction and blocking metastatic aggression of B16/F10 melanoma
Автор: Kit O.I., Frantsiyants E.M., Shikhlyarova A.I., Neskubina I.V., Kaplieva I.V., Cheryarina N.D., Vereskunova A.A., Trepitaki L.K., Pogorelova Y.A., Bandovkina V.A., Surikova E.I., Kachesova P.S., Sheiko E.A., Kotieva I.M., Gusareva M.A., Luganskaya R.G., Bosenko E.S.
Журнал: Cardiometry @cardiometry
Рубрика: Review
Статья в выпуске: 22, 2022 года.
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The aim is to evaluate the physiological parameters of the efficacy of cardiac mitochondria transplantation in male mice with chronic neurogenic pain and B16/F10 melanoma growth. Materials and methods. Male mice (n=37) of the C57BL/6 line were used in the research work. The animals covered experimental groups as follows: mice with chronic neurogenic pain (CNP) + B16/F10 melanoma (n=27); mice with CNB + B16/ F10 melanoma + mitochondrial therapy (MC therapy) (n=10). Mitochondria were isolated from the heart of an intact rat with the use of differential centrifugation. An introduction of mitochondria to mice was carried out daily intraperitoneally at a dose of 3.3 mg of protein for 3 weeks. Statistical analysis of the results is carried out with the Statistica 10.0 software. Results. On day 21 (week 3) of the experiment, macroscopically in the melanoma tissue in the group of animals with MC therapy, there were 2.5 times more necrosis cases than in the group without MC therapy. During the examination of the internal organs, no metastases were detected in the animals treated with MC therapy, while in 100% of the animals without MC therapy metastases were found in the lungs and in 95% of them in the spleen. In the animals received MC therapy, there was no damage to the heart muscle in 75% of the cases, while in the group of the animals without MC therapy, the presence of lesions in the form of bruises on the surface of the heart was macroscopically detected in 100% of the animals. Conclusion. Thus, intraperitoneal transplantation of intact heart mitochondria contributed to the prevention of myocardial infarction and metastases to internal organs in the C57BL/6 male mice with B16/F10 melanoma growing against the background of chronic neurogenic pain.
Mitochondrial therapy, mitochondria, heart, myocardial infarction, b16/f10 melanoma, chronic neurogenic pain, male mice
Короткий адрес: https://sciup.org/148324635
IDR: 148324635 | DOI: 10.18137/cardiometry.2022.22.5055
Список литературы Biological effects of mitochondrial therapy: preventing development of myocardial infarction and blocking metastatic aggression of B16/F10 melanoma
- Virani SS, et al. Heart Disease and Stroke Statistics-2020 Update: A Report From the American Heart Association. Circulation. 2020;141:e139–e596. doi: 10.1161/CIR.0000000000000757.
- Brown DA, et al. Expert consensus document: Mitochondrial function as a therapeutic target in heart failure. Nat. Rev. Cardiol. 2017;14:238–250. doi: 10.1038/nrcardio.2016.203.
- Bonora M, et al. Targeting mitochondria for cardiovascular disorders: Therapeutic potential and obstacles. Nat. Rev. Cardiol. 2019;16:33–55. doi: 10.1038/s41569-018-0074-0.
- Cao JL, et al. Role of mitochondrial Ca2+ homeostasis in cardiac muscles. Arch. Biochem. Biophys. 2019; 663: 276–287. doi: 10.1016/j.abb.2019.01.027.
- Kohlhaas M, Nickel AG, Maack C. Mitochondrial energetics and calcium coupling in the heart. J. Physiol. 2017; 595:3753–3763. doi: 10.1113/JP273609.
- Clark MA, Shay JW. Mitochondrial transformation of mammalian cells. Nat. Cell Biol. 1982;295:605–607. doi: 10.1038/295605a0.
- Kitani T, et al. Direct Human Mitochondrial Transfer: A Novel Concept Based on the Endosymbiotic Theory. Transplant. Proc. 2014; 46: 1233–1236. doi: 10.1016/j.transproceed.2013.11.133.
- Katrangi E, et al. Xenogenic transfer of isolated murine mitochondria into human rho0 cells can improve respiratory function. Rejuvenation Res. 2007;10:561–570. doi: 10.1089/rej.2007.0575.
- Kit ОI, et al. Influence of B16/F10 melanoma development variant on BCL-2 content in mitochondria of cells of various organs of female mice. Bulletin of Siberian Medicine. 2021;20(3):46-53. [in Russian]
- Kit OI, et al. The processes of self-organization of mitochondria during the growth of experimental tumors under conditions of chronic neurogenic pain. News of higher educational institutions. North Caucasian region. Series: Natural Sciences. 2019; 2 (202):97-105. [in Russian]
- Leppert W, Zajaczkowska R, Wordliczek J, Dobrogowski J, Woron J, Krzakowski M, Pathophysiology and clinical characteristics of pain in most common locations in cancer patients. J. Physiology and Pharmacology. 2016. 67(6). Р. 787-799.
- Kit OI, et al. The effect of chronic neuropathic pain on the course of the malignant process of B16/F10 melanoma in male mice. News of higher educational institutions. North Caucasian region. Series: Natural Sciences. 2019; 1 (201):106-11. [in Russian]
- Frantsiyants EM, et al. Content of apoptosis factors and self-organization processes in the mitochondria of heart cells in female mice C57BL/6 under growth of melanoma b16 / f10 linked with comorbid pathology. Cardiometry. 2021; 18:121-30.
- Frantsiyants ЕМ, et al. The functional state of mitochondria of cardiomyocytes in a malignant process against the background of comorbid pathology in the experiment. South Russian journal of oncology. 2021; 2( 3):13-22. [in Russian]
- Kotieva I.M., et al. An increase in the degree of malignancy of melanoma against the background of chronic pain in female mice. Malignant tumors. 2017;7( 3S1):123-124. [in Russian]
- Egorova M.V., Afanasiev S.A. Isolation of mitochondria from cells and tissues of animals and humans: Modern methodological techniques. Siberian medical journal. 2011; 26(1-1):22-28 [in Russian]
- Levitsky S, et al. Mitochondrial DNA deletions in coronary artery bypass grafting patients. Eur. J. Cardiothorac. Surg. 2003; 24: 777–784. doi: 10.1016/S1010-7940(03)00501-3.
- McCully JD, et al. Injection of isolated mitochondria during early reperfusion for cardioprotection. Am. J. Physiol. Circ. Physiol. 2009; 296: H94–H105. doi: 10.1152/ajpheart.00567.2008.
- Cowan DB, et al. Intracoronary Delivery of Mitochondria to the Ischemic Heart for Cardioprotection. PLoS ONE. 2016; 11:e0160889. doi: 10.1371/journal.pone.0160889.
- Emani SM, et al. Autologous mitochondrial transplantation for dysfunction after ischemia-reperfusion injury. J. Thorac. Cardiovasc. Surg. 2017; 154: 286–289. doi: 10.1016/j.jtcvs.2017.02.018.
- Ramirez-Barbieri G, et al. Alloreactivity and allorecognition of syngeneic and allogeneic mitochondria. Mitochondrion. 2019; 46:103–115. doi: 10.1016/j.mito.2018.03.002.