Brassinosteroids denigrate the seasonal stress through antioxidant defense system in seedlings of Brassica juncea L

Автор: Kumar Sandeep, Sirhindi Geetika, Bhardwaj Renu, Kumar Manish

Журнал: Журнал стресс-физиологии и биохимии @jspb

Статья в выпуске: 2 т.10, 2014 года.

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The present work has been undertaken to study the effect of exogenously application of 24-epiBL and 28-homoBL on soluble protein, proline contents and antioxidant defense system of Brassica juncea L. RLM 619 under the influence of seasonal stress. It was observed that 24-epiBL and 28-homoBL treatment enhance the soluble protein, dry weight and shoot length of B. juncea seedlings under seasonal stress. If seeds treated with the different concentrations (10 -6, 10 -8 and 10 -10 M) of 24-epiBL and 28-homoBL revealed batter growth, protein and proline contents as compare to untreated seedlings. Similarly the activities of antioxidant enzymes SOD, CAT, APOX, DHAR, PPO and Auxinases were enhanced by the application of different concentration of both brassinosteroids, whereas MDA content was decrease with both brassinosteroids treatments. Then we have concluded that both brassinolides have the seasonal stress ameliorative properties in B. juncea seedlings grown under the influence of seasonal stress. This study culminates to the role of brassinolides as an anti-stress property for protection of plant from various types of stresses.

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Antioxidant enzymes, brassica juncea, brassinosteroids, ros, seasonal stress

Короткий адрес: https://sciup.org/14323877

IDR: 14323877

Список литературы Brassinosteroids denigrate the seasonal stress through antioxidant defense system in seedlings of Brassica juncea L

  • Aebi, H. (1983) Catalase. In: Methods of Enzymatic Analysis, eds Bergmeyer H.U. Verlag Chemie, Weinhan. Pp 673-684
  • Bastin, Unleur, O. (1972) Effect of actinomycin-D on the formation of enzymes in Jerusalem artichoke tuber slices. Planta, 102, 357-361
  • Bates, L.S., Waldren, R.P., and Teare, I.D. (1973) Rapid determination of free proline for water-stress studies. Plant Soil, 39, 205-207
  • Cao, S., Xu, Q., Cao, Y., QiAn, K., An, K., Zhu, Y., Binzeng, H., Zhao, H. and Kuai, B. (2005). Loss-of-function mutation in DET2 gene lead to an enhanced resistance to oxidative stress in Arabidopsis. Physiol. Plant, 123, 57-66
  • Clouse, S.D. and Sasse, J.M. (1998). Brassinosteroids: Essential regulators of plant growth and developments. Annu. Rev. Plant. Physiol. Plant Mol. Biol., 49, 427-451
  • Dalton, D.A., Russell, S.A., Hanus, F.J., Pascoe, G.A. and Evans, H.J. (1986) Enzymatic reactions of ascorbate and glutathione that prevent peroxide damage in soybean root nodules. Proc. Natl. Acad. Sci. USA, 83, 3811-3815
  • Darbyshire, B. (1971) The effect of water stress on Indole acetic acid oxidase in Pea plants. Plant Physiol., 47, 65-67
  • Dubois, M., Gills, K. A., Hamilton, J. K., Robers, P.A. and Smith, F. (1956). Colorometric methods for the determination of sugars and related substances. Ann. Chem., 28, 350-356
  • Gordon, S.A., Weber, R.P. (1951) The effect of x-radiation on indoleacetic acid and auxin levels in the plant. Ameri. Jour. of Bot., 37, 78
  • Hayat, S., Ali, B., Aiman, H.S. and Ahmad, A. (2007). Brassinosteroids enhanced antioxidants under cadmium stress in Brassica juncea. Environ. Exp. Bot. 60, 33-41
  • Heath, R., Packer, L. (1968) Photo peroxidation in isolated chloroplasts I Kinetics and stoichiometry of fatty acid and peroxidation. Arch. Biochem. Biophys., 196, 385-395
  • Hernández, J.A., Jimenez, A., Mullineaux, P. and Sevilla, F. (2000) Tolerance of pea (Pisum sativum L.) to long-term salt stress is associated with induction of antioxidant defenses. Plant Cell Environ., 23, 853-862
  • Hind, G., Marshak, D. and Coughalan, S. (1995) Spinach thylakoid polyphenol oxidase: cloning, characterization, and relation to a putative protein kinase. Biochem., 34, 8157-8164
  • Kono, Y. (1978) Generation of superoxide radical during autooxidation of hydroxylamine and an assay for superoxide dismutase. Archives of Biochem. Biophys.,186, 189-195
  • Kovtun, Y., Chiu, W.L. and Tena, S.J. (2000) Functional analysis of oxidative stress -activated mitogen -activated protein kinase cascade in plants. Proc Natl. Acad. Sci. USA., 97, 2940-2945
  • Krishna, P. (2003) Brassinosteroids-mediated stress responses. Jour. Plant Growth Regul., 22, 289-297
  • Lowry, O.H., Rosenbrough, N.J., Farr, A.L. and Randall, R.J. (1951) Protein measurement with folin-phenol reagent. The Jour. of Biol. Chemis., 193, 265-275
  • Mazorra, L.M., Nunez, M., Hechavarria, M., Coll, F. and Sanchez-Blanco, M.J. (2002) Influence of brassinosteroids on antioxidant enzymes activity in tomato under different temperature. Biol. Plant. 45, 593-596
  • Mc Cord, J.M. (2000) The evolution of free radicals and oxidative stress. Am. Jour. Med. 108, 652-659
  • Metwally, A., Finkemeier, I., Georgi, M. and Dietz, K.J. (2003). Salicylic acid alleviates the cadmium toxicity in barley seedlings. Plant Physiol. 132, 272-281
  • Mittler, R. (2002) Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science, 7, 405-410
  • Nakano, Y. and Asada, K. (1981) Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplast. Plant and Cell Physiol., 22(5), 867-880
  • Noctor, G. and Foyer, C.H. (1998) Ascorbate and glutathione: Keeping active oxygen under control. Annu. Rev. Plant Physiol. Plant Mol. Biol. 49, 249-279
  • Nunez, M., Mazzafera, P., Mazorra, L.M., Siqueira, W.J. and Zullo, M.A.T. (2003). Influence of brassinosteroid analogue on antioxidant enzymes in rice grown in culture medium with NaCl. Biol. Plant, 47, 67-70
  • Ozdemir, F., Bor, M., Demiral, T. and Turkan, I. (2004) Effects of 24-epibrassinolide on seed germination, seedling growth, lipid peroxidation, proline content and antioxidative system of rice (Oryza sativa L.) under salinity stress. Plant Growth Regul., 42, 203-211
  • Rachkovskaya, M.M., and Kim, L.O. (1980) Changes in activities of some oxidases as an index of plant adaptation to industrial pollutants, Gazoustoichivost' rastenii (Plant Tolerance to Gases), Nikolaevskii, V.S., Ed., Novosibirsk: Nauka
  • Rao, S.S.R., Vardhini, B.V., Sujatha, E. and Anuradha, S. (2002). Brassinosteroids. A new class of phytohormones. Curr. Sci. 1239-1245
  • Sairam, R.K. (1994) Effect of homobrassinolide application on plant metabolism and grain yield under irrigated and moisture stress condition of two wheat varieties. Plant Growth Regul., 14, 173-181
  • Schilling, G., Schiller, C. and Otto, S. (1991) Influence of brassinosteroid on organ relations and enzyme activities of sugar beet plants. In: Brassinosteroids: Chemistry, Bioactivity and application eds. Cutlar HG, Yokata T and Adam G. ACS Symp Ser 474. Washington, DC: Am. Chem. Soc., 208-219
  • Schützendübel, A. and Polle, A. (2002) Plant responses to abiotic stresses: heavy metal-induced oxidative stress and protection by mycorrhization. Jour. of Exp. Bot., 53, 1351-1365
  • Sharma, P. and Bhardwaj, R. (2007). Effects of 24-Epibrassinolide on growth and metal uptake in Brassica juncea L. under copper metal stress. Acta Physiol. Planta., 29, 259-263
  • Sirhindi, G., Kumar, S., Bhardwaj, R. and Kumar, M. (2009) Effect of 24-epibrassinolide and 28-homobrassinolide on antioxidant defense system in seedlings of Brassica juncea L. Physiol. Molecul. Biol. Plants, 15(4), 35-41
  • Steffens, J.C., Harel, E. and Hunt, M.D. (1994). Polyphenol oxidase. In: Recent Advances in Phytochemistry, genetic engineering of plant secondary metabolism. eds. Ellis BE, Kuroki GW, Stafford HA, Plenum Press, New York. 28 275-312
  • Trebst, A. and Depika, B. (1995). Polyphenol oxidase and photosynthesis research. Photosyn. Res., 46, 41-44
  • Vahala, J., Keinanen, M., Schutzendubel, A., Polle, A. and Kangasjarvi, J. (2003) Differential effects of elevated ozone on two hybrid aspen genotypes predisposed to chronic ozone fumigation role of ethylene and salicylic acid. Plant Physiol., 132, 196-205
  • Voskresenskaya, O.L., Chernavina, I.A. and Aksenova, V.A. (1986). Effect of zinc surplus on iron accumulation and activity of iron-containing enzymes in oat. Plant Physiol., 33, 1056-1060
  • Zhukova, L.A., Populyatsionnaya, Z. and Lugovykh, R. (1995) Population Life of Meadow Plants, Yoshkar-Ola: Mar Gos Univ
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