In vitro release studies of rilpivirine from in situ forming polymeric implants in buffer solution and in a gel phantom of muscle tissue
Автор: Ulianova Y.V., Ermolenko Y.V., Vanchugova L.V., Mityukov A.V., Gelperina S.E.
Журнал: Вестник Воронежского государственного университета инженерных технологий @vestnik-vsuet
Рубрика: Пищевая биотехнология
Статья в выпуске: 4 (98) т.85, 2023 года.
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Development of in situ forming implants (ISFI) based on PLGA polymers is one of the most promising approaches to long-acting injectables. Evaluation of the drug release rate from such depot formulations requires methods that most closely simulate in vivo conditions. Gel phantoms mimic the elastic properties of muscle tissue and appear to be a promising replacement for conventional methods using physiologically relevant buffer solutions. Accordingly, the aim of the study was to select the optimal composition for the gel phantom formation and evaluate the effect of the phantom matrix on the release rate of rilpivirin used as a model drug from the PLGA ISFI. According to the results of the study, a 1% agarose gel was the best suited for a tissue phantom preparation and implant formation. It was also shown that the release profile of rilpivirin from the ISFI matrix depended on how the implant was formed (in a gel or freely in buffer). In the case of a phantom, the structure of the implant was less porous and retained its shape for 28 days of incubation at 37 °C. During this period, the ISFI formed in an agarose gel released considerably less rilpivirin compared to the ISFI formed without gel (11% vs 80%).
Isfi, plga, drug release, agarose gel, polyacrylamide gels
Короткий адрес: https://sciup.org/140304450
IDR: 140304450 | DOI: 10.20914/2310-1202-2023-4-70-75
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