Research the antitumor properties of a peptide consisting of a penetrating peptide fragment and a RAS-GT pase inhibitor against colon cancer (HT29) and ovarian cancer (OAW-42, OVCAR-3) cell lines

Research objective: research the antitumor activity of a peptide construct including a Ras-GTPase inhibitor and an internalized vector-penetrating peptide fragment providing effective intracellular penetration.Materials and methods. Using mathematical modeling methods, a peptide construct (K26K) has been developed, which is an inhibitor of the binding of the Ras-Raf inhibitor complex and includes a sequence that ensures effective internalization of the peptide into cells. The study was conducted in vitro using cell cultures of human tumor lines as models of malignant diseases: HT29 (human colon adenocarcinoma), OAW-42 (ovarian carcinoma, human), OVCAR-3 (human ovarian adenocarcinoma).The study was conducted by evaluating the cytotoxic and cytostatic effects of the Ras-GTPase peptide inhibitor sequence against cell lines using a number of methods: MTT test; flow cytofluorimetry; evaluation of proliferation in real time (iCELLigence system).Results. It was shown that the studied sequence (K26K) has pronounced cytostatic properties against colorectal cancer and ovarian cancer cells. It is capable of inducing apoptosis and inhibiting proliferation processes in tumor cell cultures of these localizations. Conclusion: Based on the conducted studies, it was shown that the studied chimeric sequence K26K - a peptide inhibitor of Ras-GTPase has pronounced antitumor properties against human colon cancer and ovarian cancer cell cultures.