Методы выделения и очистки ДНК из лизатов клеток (обзор)

Автор: Петров Д.Г., Макарова Е.Д., Гермаш Н.Н., Антифеев И.Е.

Журнал: Научное приборостроение @nauchnoe-priborostroenie

Рубрика: Приборостроение физико-химической биологии

Статья в выпуске: 4 т.29, 2019 года.

Бесплатный доступ

В представленном обзоре рассмотрены методы выделения и очистки ДНК из лизатов клеток, классифицированные по основным принципам и конкретным средствам, использованным с этой целью. Изложены известные на сегодняшний день преимущества, особенности использования и возможные ограничения для ПЦР-анализа. Использованные материалы включают как обзорные публикации, так и ряд "старых" работ, которые до сих пор не потеряли своей актуальности и являются основой для разработки современных нестандартных методов выделения и очистки ДНК.

Нуклеиновые кислоты, выделение ДНК, очистка ДНК, двуокись кремния, магнитные частицы, спин-колонки, жидкофазные методы, nucleic acid, DNA isolation, DNA purification, silicon dioxide, spin column, magnetic particles, chelex, liquid phase methods

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Короткий адрес: https://sciup.org/142221438

IDR: 142221438 | DOI: 10.18358/np-29-4-i2850

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