Методы выделения и очистки ДНК из лизатов клеток (обзор)

Петров Д.Г.; Макарова Е.Д.; Гермаш Н.Н.; Антифеев И.Е.

doi:10.18358/np-29-4-i2850

Научные статьи \ Математика. Естественные науки \ Химия. Кристаллография. Минералогия \ Аналитическая химия

Методы выделения и очистки ДНК из лизатов клеток (обзор)

Петров Д.Г. Макарова Е.Д. Гермаш Н.Н. Антифеев И.Е.

Журнал: Научное приборостроение @nauchnoe-priborostroenie

Рубрика: Приборостроение физико-химической биологии

Статья в выпуске: 4 т.29, 2019 года.

Бесплатный доступ

В представленном обзоре рассмотрены методы выделения и очистки ДНК из лизатов клеток, классифицированные по основным принципам и конкретным средствам, использованным с этой целью. Изложены известные на сегодняшний день преимущества, особенности использования и возможные ограничения для ПЦР-анализа. Использованные материалы включают как обзорные публикации, так и ряд "старых" работ, которые до сих пор не потеряли своей актуальности и являются основой для разработки современных нестандартных методов выделения и очистки ДНК.

нуклеиновые кислоты \ выделение ДНК \ очистка ДНК \ двуокись кремния \ магнитные частицы \ спин-колонки \ жидкофазные методы \ nucleic acid \ DNA isolation \ DNA purification \ silicon dioxide \ spin column \ magnetic particles \ chelex \ liquid phase methods

Список литературы Методы выделения и очистки ДНК из лизатов клеток (обзор)

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