Modification of the results of bone tissue bio-engineering in vivo using RGD peptides, applied to nanostructural coatings of calcium phosphate

Arginine-glycine-aspartic acids (RGD) are ones among the main participants of cell adhesion; they recognize the sequences in cell adhesion molecules, being high-affinity ligands for integrine receptors on cells, including stem cells and progenitor cells of bone marrow. In this work the regulating effect of RGD peptides on osteogenic potential of nanostructural calcium-phosphate (CP) coatings of different kinds has been examined using the test of ectopic osteogenesis (in vivo bioreactor). RGD peptides modulated distribution, functional activity and differentiation of bone marrow stromal and hemopoietic cells. Nevertheless, they inhibit the adhesion of bone marrow stem cells, and impair bone tissue remodeling. This fact may restrict their prospects for bone tissue engineering on CP coatings.