Targeted catalysis as a way to increase the yield of biological valuable substances from fruit and berry raw materials

The rational method of plant tissue treatment is a particularly important aspect to extract and preserve natural value of biologically active components. The aim of this work was development of biotechnology of semi-finished products with an increased content of food and biologically active substances as beverages base with a balanced composition production. The quantitative and qualitative compound of biologically active components in the obtained fermentolysates and concentrates by various spectrophotometric, colorimetric and titrimetric analysis methods was investigated. The possibility of increasing of biologically active components yield in extracts as a result of targeted enzymatic hydrolysis of cell walls polymers of fruit and berry raw materials was identified. It was established that multienzyme compositions consisting of pectinase, protease, β-glucanase for apple raw materials, and pectinase and β-glucanase for blackcurrant raw materials treatment provide increasing the yield of phenolic substances, vitamin C and reducing carbohydrates. So, the concentration of phenolic substances in the blackcurrant fermentolysate was increased 7.5 times, and vitamin C - 2 times, in contradistinction to juice. The possibility of receiving concentrated forms of ingredients by using modern membrane technologies was experimentally shown. The optimal degree of fermentolysates concentration to maintain organoleptic characteristics and the content of biologically active substances was selected. Soluble solids content of blackcurrant concentrate was 64.8%, apple concentrate - 58.2 %. An experimental batch was produced and biochemical composition of concentrates from fruit and berry raw materials was studied. Produced ingredients will be used as a base for creating natural beverages with balanced composition.