On the morphological properties of the strain S. cerevisiae Y-503 in the conditions of osmotic, temperature and acid stress

The article presents the results of the influence of osmotic, temperature and acid stress on the morphological and cultural characteristics of the strain S. cerevisiae Y-503, obtained as a result of laser exposure. The concentration of NaCl (5%, 10%, 15%, 20%) was varied at different pH values (3.0; 4.5; 7.0; 9.0; 11.0) and temperatures (30°C and 37°C). Long lasting cultivation of strain Y-503 was performed without prior adaptation. The optimal conditions for maintaining cell culture viability in the absence of NaCl in the medium were determined with the following sequence of values of pH 4.5> pH 3.0> pH 7.0> pH 9.0> pH 11.0 (30°C) and pH 3.0> pH 11.0> pH 7.0> pH 9.0> pH 4.5 (37°C); and with 5% NaCl at pH 9.0> pH 4.5> pH 7.0> pH 3.0> pH 11.0 (30°C) and pH 7.0> pH 11.0> pH 3.0> pH 9.0> pH 4.5 (37°C). It was shown that for all stress factors, an increase in temperature led to an increase in budding cells, the formation of inclusions and spores. The research results can be used in the development of biotechnologies for various purposes, based on the use of S. cerevisiae yeast resistant to extreme conditions.