Assessment of bioavailability of BAS in the composition of bioactive emulsion food systems

The high efficiency of delivery of biologically active substances (BAS) using various systems in the nutritional correction of non-infectious diseases of the population has been confirmed by a number of studies. However, an important aspect is to confirm the bioavailability after consumption of each biologically active substance contained in the food matrix, including through preclinical studies. To deliver biologically active substances in this study, a bioactive emulsion food system was used. A bioactive emulsion food system was used as a BAS delivery system in this study. The purpose of the study was a comparative assessment of the bioavailability of vitamin B12 in the blood after the introduction of a bioactive emulsion food system and a direct emulsion enriched with this vitamin in experiments on laboratory animals. The studies were conducted on white laboratory male Wistar rats weighing 350 g ± 10 %, aged 10-11 weeks in 2 stages: a pilot experiment to determine the optimal period of bioavailability of vitamin B12 (after 1, 2, 3 or 4 hours) and the main experiment with selected bioavailability period of 4 hours based on the results of a pilot experiment. The quantitative content of vitamin B12 in the blood of laboratory animals that received a bioactive emulsion food system (BEPS) was 10.4 % higher than its content in the blood of laboratory animals that received a direct emulsion with this vitamin. The control group of animals that received an emulsion without vitamin was characterized by a content of vitamin B12 in the blood that was 33 % lower than the group that received BEPS. The results of the study showed that the encapsulation of vitamin B12 in the internal aqueous phase of BEPS fortificate ensures its greater bioavailability. The results of the study showed that the encapsulation of vitamin B12 in the internal aqueous phase of BEPS fortificate ensures its greater bioavailability. The results of a general blood test indicate the occurrence of processes in the animal’s body that, to varying degrees, contribute to improving the body’s resistance to various factors and the formation of new cells.