Assessment of wound surface and tissue regeneration using chlorophyll and hemin in free and polymeric forms

The aim of the study is to comparatively analyze the reparative properties of chlorophyll (Chl), the ac-tive ingredient in nettle extract, and hemin in free and polymeric forms, using an experimental incised wound model. The experiment was designed to study wound regeneration by secondary intention. The study was conducted on white, nonlinear mice matched for similar weight, age, and gender and qua-rantined for 7 days. Wound area was analyzed, and the regeneration process was monitored. Therapy began on the first postoperative day. 200 μl of the preparations were applied daily. The animals' general condition, motor activity, and wound surface condition were assessed by phases of progression (inflamma-tion, regeneration, and epithelialization) using the planimetric method proposed by J.I. Kundin. Polymeric forms of Chl and hemin induced high-quality tissue reparation, shortening the regeneration time. A high degree of reparation was observed in the 1st and 3rd experimental groups, with normotrophic healing. A moderate degree of healing was observed in the 2nd and 4th groups. On the 7th day, the 1st group showed the most intense reduction in wound surface area of all experimental groups – 47 %. In the 2nd experimental group, wound area decreased by 43.6 %, with wounds without eschar formation and low granulation. In the third experimental group of mice, an increase in wound surface area by 14 % (within the error limit) was observed. In the fourth experimental group, a significant decrease in wound surface area was 6.6 %. By day 21, the wound surface of mice in both experimental groups had completely epithe-lialized. Polymeric forms of Chl and hemin demonstrated high regenerative activity in this experiment. The obtained data suggest their potential use in the treatment of farm animals.