Determining the preventive agent protective effect on the kidney ultrastructure of rats and rabbits under combined mycotoxicosis: a morphometric aspect

The paper reflects the results of a morphometric analysis of the action of the developed preventive complex in relation to the preservation of the ultrastructure of kidney podocytes in rats and rabbits with combined mycotoxicosis. The purpose of the study is to determine the protective effect of the developed preventive complex, which includes the natural mineral halloyasate (previously not used in the Russian Federation for mycotoxicosis), on the ultrastructure of podocytes in rats and rabbits under conditions of subacute intoxication with mycotoxins (T-2, aflatoxin B1, zearalenone). The experiment took place over 21 days on 40 white non-linear rats with a live weight of 150-170 g and 40 chinchilla rabbits with a live weight of 1.7-1.9 kg. From animals of each species, according to the principle of analogues, 4 groups of 10 animals each were formed (biological control, toxic control, basic diet with a preventive complex, toxic diet with a preventive complex) taking into account age, sex and body weight. Kidney tissue samples were examined using a transmission electron microscope using the ultrathin section technique. As a result of statistical analysis of the morphometric data obtained in the experiment, differences in the width of cytopodia were identified between the prevented group and the biological control group, on the one hand, and the toxic control group, on the other. As a result, it was concluded that the developed preventive complex is effective when administered orally in conditions of combined T-2, afla-, zearalenone mycotoxicosis in laboratory animals (rats and rabbits), without having a negative effect on the ultrastructure of the kidneys.