The main trends in dynamics of soil microbiom during a long-term field experiment as indicated by high throughput sequencing the 16S-rRNA gene libraries

A biodiversity of soil microbial communities now is being widely studied. Since 1912, the influence of different agrotechnical factors on soil is being examined in the stationary field experiment in the Moscow Agricultural Academy. We investigated an effect of different combination of three factors, i.e. plants (flax and winter rye), crop rotation and soil acidity, to the soil microbiom. To estimate a biodiversity, the throughput sequencing was used. Also an original approach, the binary sampling, was suggested to analyze the data of throughput sequencing. It was shown the number of microorganisms which changed under the influence of studied factor is approximately equal to those staying unchanged. But a taxonomic composition of these two groups differed, and the impacted group was more uniform. Thus, we can conclude that the less part of microorganism is sensitive to the acting factors, and the rest ones keep their number unchanged, more or less. The taxonomic groups of microorganisms, sensitive to each of studied factors, were revealed. The binary sampling, suggested herein, allows finding out a relation between an action of some ecological factor and varying taxonomic structure of microbial population.