Initial Results of a Study of the Biotechnological Potential of Epiphytic Bacteria Isolated from Anthriscus Sylvestris

The purpose of the study is to identify promising bacterial strains for agricultural production in the Non–Chernozem Zone of Russia. The species chosen for the bacterial extraction has been a wild chervil (Anthriscus sylvestris), a species with high environmental stability and the ability to spread rapidly. In 2025, as part of the work, a field trip was conducted to a meadow with A. sylvestris thickets (site coordinates: 59.296602, 38.220652). Plant samples of various organs of this species have been collected, and 27 bacterial isolates (13 isolates from the rhizosphere and 14 ones from the phyllosphere) have been isolated in the laboratory. The following properties of the strains have been evaluated: the ability to synthesize IAA (indoleacetic acid), fix molecular nitrogen, transform phosphates into a form accessible to plants, and exhibit cellulose, protein, and amylolytic activities. The isolate KR-08-25 has had the highest IAA content (123.9 mg/l). At the same time, 44% of isolates (15 pcs.) have possessed the ability to transform phosphates, fix molecular nitrogen, and synthesize IAA. The most active isolate in relation to cellulose has been KR-07-25, and the most active isolates in relation to starch hydrolysis have been KF-01-25, KF-02-25, KF-03-25, and KR-09-25. In addition, free amino acids in the culture fluid of promising isolates have been assessed. KF-10-25 has had the highest free amino acid content, reaching 421.80 mg/l. A very promising isolate has been KR-08-25, which has stimulated the germination of water cress seeds by 12% relative to the control.