Selection of the optimal nutrient medium for cultivation of microalgae Chlorella kessleri VKPM AI-11 ARW in laboratory conditions

We carried out the selection of the optimal nutrient medium for the cultivation of microalgae Chlorella kessleri in laboratory conditions. According to the patent information, to grow C. kessleri microalgae of the VKPM AI-11 ARW strain (Russian Federation, NPO Algobiotechnology), it is necessary to use a complex nutrient medium consisting of solutions of four chemical reagents and a centrate of distillery stillage. The results of algae culturing on the proposed medium demonstrated good growth rate with minimal time to achieve optimal optical density (5 days) and enter the exponential phase (3 days). Based on the data obtained during the study, the Tamiya medium at a concentration of 30% without additional application of trace elements will be the most suitable for cultivation in the laboratory and optimal one, taking into account practical aspects such as simplicity and cost. Similar results were obtained during cultivation on this medium: the time to achieve optimal optical density was 5 days, and the time to enter the exponential phase was 3 days.