Kappa-casein and diacylglycerol o-acyltransferase gene polymorphism in black-and-white cattle

Polymorphism has been studied and occurrence frequency of genotypes and alleles by genes of kappa-casein and diacylglycerol O-acyltransferase in first-calf heifers and highly productive black-and-white cows of different genealogical lines has been determined. The highest frequency of CSN3 AB genotype (34.7% and 38.9%) and CSN3 BB genotype (10.2 and 5.6%) has been registered in the first-calf heifers of M. Chiftein and R. Sowering line at the B allele frequency of CSN3 gene - 0.28 and 0.25. Highly productive cows also have high frequency of desirable genotypes in similar two lines, respectively, CSN3 AB - 47.3 and 36.2%, CSN3 BB - 5.4% and 7.2%, with B allele frequency - 0.25-0.29. DGAT1 gene has the best frequency of DGAT1 AA genotype in primary B.B. Aidial (65.9%) and M. Chiftein (65.3%) lines, with good occurrence of the desired K gene allele of DGAT1 0.35 and 0.39, respectively. High frequency of DGAT1 AK genotype (60.7%), DGAT1 KK (9.8%) and the highest frequency of K allele - 0.40 have been established for highly productive cows of V.B. Aidial line. In the black-and- white cattle there has been revealed the advantage of A allele over B allele in the kappa-casein gene, and the advantage of A allele over K allele in the diacylglycerol O-acyltransferase gene in all analyzed genealogical lines.