Development of a methodical approach to the evaluation of the quality and safety of platelets by the method of dynamic light scattering

Donor blood and its components may contain cell fragments from various cells, primarily from activated platelets. Acting as innate immune cells and major participants in the blood clotting process, platelets change shape and generate microparticles. Platelet microparticles enhance the formation of intercellular contacts and promote inflammatory responses. Interacting with leukocytes, platelet microparticles increase their adhesiveness, activate the process of interaction of neutrophil granulocytes with both endothelial cells and platelets. In inflammatory and thrombotic conditions, the number of formed microparticles increases significantly. The formation of platelet microparticles contributes to immune responses because of their high content of pro-inflammatory cytokines, various chemokines and lipid mediators. The detection of microparticles based on dynamic light scattering (DLS) will allow us to distinguish activated (high microparticle content) from inactivated (low microparticle content) platelets during transfusion and optimize the use of this scarce blood component. Rational approach to platelet concentrates transfusion taking into account the degree of activation will allow to reduce the volume of procurement and the total number of prophylactic CT transfusions, to differentiate deficient components for prophylactic and therapeutic transfusions.