Research of camel shanks proteinhydrolyzates by electrophoregram
Автор: Satayeva Zh. I., Tayeva A. M.
Журнал: Вестник Алматинского технологического университета @vestnik-atu
Рубрика: Техника и технологии
Статья в выпуске: 3 (128), 2020 года.
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This research aims to study the degree of hydrolysis, determining the nature of protein hydrolyzates, which determine their size and molecular weight by the method of electrophoregram. In this research, a camel pancreas suspension was used to hydrolyze proteins from camel shanks. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to monitor the distribution of proteins and evaluate their molecular weights at different incubation times. Electrophoregram processing using the BioCapt program (Vilber Lourmat, France) determines the nature of the hydrolysis of protein and peptide profiles among hydrolyzates and the hydrolysis time for 8 hours shows the most significant accumulation of low molecular weight compounds with a molecular mass of
Amel shanks, pancreas, electrophoregram, protein hydrolyzates, low molecular weight peptides
Короткий адрес: https://sciup.org/140250907
IDR: 140250907 | DOI: 10.48184/2304-568X-2020-3-67-73
Список литературы Research of camel shanks proteinhydrolyzates by electrophoregram
- Boland M.J., Rae A.N., Vereijken J.M., Muwissen P.M., Fisher Ar.R.H., van Boekel M.A.J.S. et al. (2013) The future supply of animal-derived protein for human consumption. Trends Food Sci & Technology 29(1), 2013. - PP. 62-73.
- da Silva, R.R. Bacterial and Fungal Proteolytic Enzymes: Production, Catalysis, and Potential Applications. Applied Biochemistry Biotechnology. 2017 Sep;183(1):1-19.
- Chakrabarti S, Guha S, Majumder K. Food-Derived Bioactive Peptides in Human Health: Challenges and Opportunities. Nutrients. 2018; 10(11):1738. Published 2018 Nov 12.
- Martínez-Alvarez, O., Chamorro, S., Brenes, A. (2015) Protein hydrolysates from animal processing by-products as a source of bioactive molecules with interest in animal feeding: A review. Food Research International, 73. - PP. 204-212.
- Kristinsson H.G., Rasco B.A. Biochemical and Functional Properties of Atlantic Salmon (Salmo Salar) Muscle Proteins Hydrolyzed with Various Alkaline Proteases. J Agricultural Food Chemistry.- 2000;48(3). - PP. 657-666.
- Dieterich F., Boscolo W.R., Pacheco Bertoldo M.T., et al. Development and characterization of protein hydrolysates originated from animal agro-industrial byproducts. J Dairy Veterinary & Animal Research 2014;1(2):56-61.
- Bhat, Zuhaib & Kumar, Sunil & Bhat, Nina. (2015). Bioactive peptides from the egg: A review. Nutrition & Food Science. 45, 190-212.
- DOI: 10.1108/NFS-10-2014-0088
- Aspevik, T., Oterhals, Å.,Rønning, S.B. et al. Erratum to: Valorization of Proteins from Co- and By-Products from the Fish and Meat Industry. Top Curr Chem (Z) 375, 57 (2017).
- DOI: 10.1007/s41061-017-0146-3
- Gómez-Guillén, M.C.; Giménez, B., López-Caballero M.E., Montero M.P. Functional and bioactive properties of collagen and gelatin from alternative sources: A review. Food Hydrocolloids.- 2011 Dec;25(8). - PP. 1813-1827.
- Choi, D.; Min S.-G.; Jo Y.-J. Functionality of porcine skin hydrolysates produced by hydrothermal processing for the liposomal delivery system. Journal of Food Biochemistry. 2018.- 42(1). - e12464.
- DOI: 10.1111/jfbc.12464
- Khiari Z.; Ndagijimana M.; Betti M. Low molecular weight bioactive peptides derived from the enzymatic hydrolysis of collagen after isoelectric solubilization/precipitation process of turkey by-products. Poultry Science.- 2014. - 93(9). - PP. 2347-2362.
- DOI: 10.3382/ps.2014-03953
- Berdutina A.V. Development of the technology of protein hydrolysates from secondary raw materials of the meat industry. PhD. D. thesis. - M., 2000. - 186 p.
- Surnin E.V. Development of technology for herodietic sausages enriched with biologically active ingredients from pork legs// PhD. D. thesis. - M., 2001. - 137 p.
- Chen N., Yang H., Sun Y., Niu J., Liu S. Purification and identification of antioxidant Peptides from walnut (Juglans regia L.) protein hydrolysates. Peptides. - 2012 Dec;38(2). - PP. 344-349.
- Li B., Chen F., Wang X., Ji B., Wu Y. Isolation and identification of antioxidative peptides from porcine collagen hydrolysate by consecutive chromatography and electrospray ionization-mass spectrometry //Food Chemistry. 2007; 102(4). - PP. 1135-1143.
- "Endopeptidase". Egyptian Journal of Medical Human Genetics, Elsevier. Britannica, edition of the encyclopedia. "Proteolytic enzyme". Encyclopedia Britannica, Encyclopedia Britannica, Inc. May 31. - 2
