Study of Physio-Morphological and Biochemical Changes during Different Developmental Stages in Mungbean [Vigna radiata (L.) Wilczek] Under Natural Environmental Conditions

The experiments were conducted to determine the effect of ageing [different developmental stages (15days, 35days and 50 days)] as a stress on growth and biochemical parameters in Vigna radiata (L.) Wilczek (mungbean) plant. The plants were uprooted and collected for observations on the 15th day, 35th day and 55th day after sowing. In natural environmental condition the plant grows naturally and its morphological parameters like shoot length, root length, leaf area, fresh weight and dry weight increased throughout. Photosynthetic pigment like chlorophyll content increased throughout but the carotenoid content decreased in aged plant tissues. Antioxidant enzymes like peroxidase activity significantly increased but catalase activity decreased as plants get older. The protein content and osmoprotectant proline content get slightly decreased in 35 DAS plant. As the plant ages, it undergoes significant internal changes driven by stress and maturation.