Changes in the parameters of pro-oxidant - antioxidant balance in the brain of rats with stepwise incomplete cerebral ischemia
Автор: Bon E.I., Maksimovich N.Ye., Dremza I.K., Kokhan N.V., Burak I.N.
Журнал: Саратовский научно-медицинский журнал @ssmj
Рубрика: Патологическая физиология
Статья в выпуске: 4 т.18, 2022 года.
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Objective: to evaluate the activity of oxidative stress in rats with stepwise incomplete cerebral ischemia (SICI). Material and methods. Experiments were performed on 24 male outbred rats weighing 260±20 g, allocated into 3 subgroups (6 animals in each) based on time of ligation of both common carotid arteries (CCA). The control group comprised of sham-operated rats of the same sex and similar weights (n=6). To identify the pro-oxidant - antioxidant state of the brain based on its homogenates, the activity of lipid peroxidation processes, the content of thiobarbituric acid reactive substances (TBARS), the concentration of reduced glutathione (GSH), total thiol groups (TSH), and the activity of glutathione peroxidase were determined. Results. SICI with ligation interval of both CCAs of 1 day and 3 days led to a significant decrease in the total SH groups of proteins and glutathione by 30% (p=0.038) and 46% (p=0.044), respectively, TBARS concentration by 29% (p=0.038) and 31 % (p=0.043), respectively. SICI with the maximum interval between CCA ligations was manifested by less pronounced changes in the pro-oxidant - antioxidant state of the brain. Conclusion. In SICI with ligation of both CCAs 7 days apart, at which histological changes were the least pronounced, changes in the pro-oxidant - antioxidant balance were insignificant. The most pronounced disorders of the pro-oxidant - antioxidant balance in the brain were observed in the subgroup with the minimum interval between CCA ligations, which implied the highest activity of oxidative stress.
Brain homogenates, cerebral ischemia, pro-oxidant - antioxidant balance
Короткий адрес: https://sciup.org/149142949
IDR: 149142949
Список литературы Changes in the parameters of pro-oxidant - antioxidant balance in the brain of rats with stepwise incomplete cerebral ischemia
- Guo MF, Yu JZ, Ma CG. Mechanisms related to neuron injury and death in cerebral hypoxic ischaemia. Folia Neuropathol. 2011; 49 (2): 78–87.
- Stevens JL, Feelisch M, Martin DS. Perioperative oxidative stress: The unseen enemy. Anesth Analg. 2019; 129 (6): 1749–60.
- Risau W. Mechanisms of angiogenesis. Nature. 1997; (386): 671–4.
- Kaliannan K, Li XY, Wang B. Multi-omic analysis in transgenic mice implicates omega-6 / omega-3 fatty acid imbalance as a risk factor for chronic disease. Commun Biology. 2019; 2 (1): 276–80.
- Chen H, Sun D. The role of Na-K-Cl co-transporter in cerebral ischemia. Neurol Res. 2005; 27 (3): 280–6.
- Réus GZ, Carlessi AS, Silva RH, et al. Relationship of oxidative stress as a link between diabetes mellitus and major depressive disorder. Oxid Med Cell Longev. 2019; (2019): 8637970. URL: https://www.hindawi.com / journals / omcl / 2019 / 8637970 / (20 Jan 2022). DOI: 10.1155 / 2019 / 8637970.
- Khunt D, Shrivas M, Polaka S. Role of omega-3 fatty acids and butter oil in targeting delivery of donepezil hydrochloride microemulsion to brain via the intranasal route: A comparative study. Pharmacology Scientific Technology. 2020; 21 (2): 45–50.
- Butin AA. Patterns of changes in the vascular-capillary network of the cerebral cortex in response to acute cerebral ischemia. Omsk Scientific Bulletin. 2004; (26): 46–57. (In Russ.) Бутин А. А. Закономерности изменений сосудисто-капиллярной сети коры большого мозга в ответ на острую церебральную ишемию. Омский научный вестник. 2004; (26): 46–57.
- Rebrova OYu. Statistical analysis of medical data. Using the Statistica Software. Moscow: MediaSfera Publishers, 2003; 312 p. (In Russ.) Реброва О. Ю. Статистический анализ медицинских данных. Применение пакета прикладных программ Statistica. М.: МедиаСфера, 2003; 312 с.
- Chalmers GR, Roy RR, Edgerton VR. Adaptability of the oxidative capacity of motoneurons. Brain Res. 1992; 570 (1): 1–10.
- Maksimovich NE, Bon LI, Zimatkin SM. Rat brain and its response to ischemia. Grodno: Grodno State Medical University, 2020; 240 p. (In Russ.) Максимович Н. Е., Бонь Е. И., Зиматкин С. М. Головной мозг крысы и его реакция на ишемию. Гродно: ГрГМУ, 2020; 240 с.
- Bissinger R, Bhuyan AAM, Qadri SM, Lang F. Oxidative stress, eryptosis and anemia: A pivotal mechanistic nexus in systemic diseases. FEBS J. 2019; 286 (5): 826–54.
- Patt S, Sampaolo S, Théallier-Jankó A, et al. Cerebral angiogenesis triggered by severe chronic hypoxia displays regional differences. J Cereb Blood Flow Metab. 1997; 17 (7): 801–6.
- Su H, Wan C, Song A, et al. Oxidative stress and renal fibrosis: Mechanisms and therapies. Adv Exp Med Biol. 2019; (1165): 585–604.
- Taysi S, Tascan AS, Ugur MG, Demir M. Radicals, oxidative / nitrosative stress and preeclampsia. Mini Rev Med Chem. 2019; 19 (3): 178–93.
- Hauck AK, Huang Y, Hertzel AV, Bernlohr DA. Adipose oxidative stress and protein carbonylation. J Biol Chem. 2019; 294 (4): 1083–8. DOI: 10.1074 / jbc.R118.003214.
