Enzyme complex of wheat as new biosensor for glutamate determination

Автор: Kudiyarova Zh. S., Kerymkulova A.R., Lesova Zh.T., Bukenova E.A., Gilmanov M.K.

Журнал: Вестник Алматинского технологического университета @vestnik-atu

Рубрика: Естественные науки

Статья в выпуске: 4 (100), 2013 года.

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Malate dehydrogenase-glutamate oxaloacetate aminotransferase (MDh-GOT) enzyme complex (the EC) was isolated and purified from wheat seeds. It was developed the effective method of purification of EC by using of ion-exchange chromatography and gel-chromatography methods. MDh-GOT consists of two heterological subunits. The essential feature of the EC is the irreversibility of its catalyzed reactions. Michaelis constants of the EC MDh-GOT to malate, glutamate and NAD were investigated. Good stability, high sensitivity and short time for analysis (1-2 minutes) of the EC to glutamate make it very convenient for determination of concentration of glutamate.


Enzyme complex (ec), malate dehydrogenase-glutamate oxaloacetate aminotransferase (mdh-got), glutamate

Короткий адрес: https://sciup.org/140205010

IDR: 140205010

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