Quantitative content of the total phenolic compounds in the herb of Bupleurum bicaule helm

In the article we present a method for quantitation of the total flavonoids and phenolcarboxylic acids content in the aerial part of Bupleurum bicaule Helm. Optimal parameters for the extraction of these groups of biologically active substances are extractantethyl alcohol 70%; grinding degree - raw materials passing through a sieve with a hole diameter of 1 mm; extraction time - 60 min; the ratio of raw materials: extractant - 1:100 (this ratio is optimal and corresponds to exhaustive extraction). We have found optimal conditions for complexation with an alcoholic solution of aluminum chloride: 2 ml of a 2% alcohol solution of AlCl3 is sufficient for a complete reaction. For the first time, we have revealed the total content of flavonoids, phenolcarboxylic acids and tannins in the aerial part of B. bicaule from different growing areas. The content of the total content of flavonoids ranged from 3.49±0.09% to 3.54±0.12%. The total content of phenolcarboxylic acids in the B. bicaule herb is from 4.02 ± 0.11 to 4.13 ± 0.15%. The total content of tannins ranged from 10.27 ± 0.36 to 11.28 ± 0.38%.