Development of a method for quantitation of the total flavonoids content in Artemisia subviscosae Herba

The article presents a method for quantitation of the total flavonoids content in the aerial part of Artemisia subviscosa by UV-spectrophotometry. We have collected full spectral data on alcoholic extraction in a complex with aluminum chloride; the absorption maximum at a wavelength of 400 nm of the test solution corresponds to the absorption maximum of the spectrum of luteolin-7-glycoside with aluminum chloride. We have determined the optimal extraction conditions: type of extractant - 40% ethyl alcohol, grinding degree of raw materials - 1 mm, ratio of raw materials to extractant - 1:50, temperature - boiling water bath, extraction time - 30 min, twice. Aluminum chloride was used as a complexing agent (2 ml of a 2% solution); the complex was formed within 45 minutes. The total flavonoids content equivalent to luteolin-7-glycoside in A. subviscosa herba is 2.03 ± 0.04%. The relative error of the proposed method does not exceed 5%.