Evaluation of the cytotoxic effects of IgG isolated from the blood serum of schizophrenic patients

Introduction. Recent studies have demonstrated the presence of catalytic properties of class G immunoglobulins(abzymes) isolated from the blood serum of schizophrenic patients. To date, a number of abzymes with different catalytic properties, including catalase activity, have been identified in such patients. However, the biological role of cata-lytically active antibodies in the pathogenesis of the disease is not clear. Objective of this work was to reveal the effects of serum IgG in patients with schizophrenia on cell viability in a model of the glial cell line U87. Materials andMethods. Isolation of IgG preparations was performed by affinity chromatography from the blood serum of 13 patients with paranoid schizophrenia in the acute phase and 6 healthy individuals. The homogeneity of the preparationswas determined by the method of gradient electrophoresis in 4-18% PAGE. IgG catalase activity was measuredspectrophotometrically. The viability of the U87 cell culture under the influence of serum IgG was assessed using thefluorescent dyes propidium iodide and Hoechst by high-throughput screening on the CellInsight CX7 platform. Forthis, glioblastoma cells were seeded in 96-well plates and cultured with IgG at a final concentration of 0.2 mg / ml for72 hours. Results. A statistically significant (p=0.047) increase in the catalase activity of IgG antibodies in patients atthe stage of exacerbation of clinical manifestations of schizophrenia was confirmed as compared with the group ofhealthy people. No effects of IgG preparations isolated from the blood serum of schizophrenic patients on the viability of the U87 glioblastoma cell culture were found. Conclusion. It was revealed that IgG in patients with schizophrenia, which have increased catalase activity, do not have negative effects on the viability of glioblastoma cells