The activity of enzymes associated with aspartate synthesis of wheat at stress conditions
Автор: Kudiyarova Zh.s, Lesova Zh.T., Muttucumaru N., Halford N.G.
Журнал: Вестник Алматинского технологического университета @vestnik-atu
Рубрика: Естественные науки
Статья в выпуске: 5 (95), 2012 года.
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In this work were measured MDH-GOT activities in wheat leaves and grain, in the case of wheat in varieties with differing degrees of tolerance to drought stress and rust infections, growing under normal and stress conditions. We have carried out modifications and optimisations of protein extraction methods for wheat grain and wheat leaf. In this study, a high level of aspartate synthesis of drought stressed plants and rust infected wheat leaves were shown with MDH-GOT assay. We recommend using the activity of EC as enzyme marker for selection of the tolerant genotypes to biotic and abiotic stresses.
Asparagine, mdh-got, drought, rust-resistant, rust-susceptible plants, seed developing
Короткий адрес: https://sciup.org/140204974
IDR: 140204974
Список литературы The activity of enzymes associated with aspartate synthesis of wheat at stress conditions
- Lea, P.J., Sodek, L., Parry M.A.J., Shewry P.R., Halford N.G. (2007) Asparagine in plants. Ann Appl Biol 150. -P. 1-26.
- Joy, K., W.; Ireland, R., J.; Lea, P., J. (1983) Asparagine synthesis in pea leaves, and the occurrence of an asparagine synthetase inhibitor. Plant Physiol., 73. -P. 165-168.
- Snapp, S., S.; Vance, C., P. (1986) Asparagine biosynthesis in alfalfa (Medicago sativa L.) root nodules. Plant Physiol. 82. -P. 390-395.
- Rognes, S., E. (1980) Anion regulation of lupine asparagine synthetase: chloride activation of the glutamine-utilizing reactions. Phytochemistry, 19. -P. 2287-2293.
- Hughes, C., A.; Beard, H., S.; Matthews, B., F. (1997) Molecular cloning and expression of two cDNAs encoding asparagine synthetase in soybean. Plant Mol. Biol., 33. -P. 301-311.
- Sieciechowicz, K. A.; Joy, K., W.; Ireland, R., J. (1988) The metabolism of asparagine in plants. Phytochemistry 27. -P. 663-671.
- Колдасова, А.М., Гильманов, М.К., Шалахметова, Г.А., Цветкова, В. М., Колдасова Ш.M. Методы изучения ферментного комплекса МДГ-ГОАТ необратимого катаболизма глютамата семян пшеницы. -Алматы, 1999. -93с.
- Bradford, M., M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72. -P. 248-254.
- Гильманов, М.К., Фурсов, О.В., Францев, А.П. Методы очистки и изучения ферментов растений. -Алма-Ата: Наука, 1981. -91 c.
- Lima, J.D., Sodek, L. (2003). N-stress alters aspartate and asparagine levels of xylem sap in soybean. Plant science 165. -P. 649-656.
- Oaks A., Ross D.W. (1984) Asparagine synthetase in Zea mays. Canadian Journal of Botany, 62. -P. 68-73.
