Mechanisms of electromagnetic influences and effects on membrane systems in neurons and cardiomyocytes

Автор: Orlov Valery I., Rudenko Mikhail Y., Shikhlyarova Alla I., Sukhov Alexander A., Kirichenko Evgeniya Y., Filippova Svetlana Y., Zernov Vladimir A., Makedonsky Dmitry F., Mamberger Konstantin K.

Журнал: Cardiometry @cardiometry

Рубрика: Original research

Статья в выпуске: 11, 2017 года.

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Aim The aim of our studies was to discover responses of the membrane systems in neurons and cardiomyocytes as well as mechanisms of influences and effects produced by the broadband-spectrum stochastic electromagnetic radiation (BBSS EMR) on them according to data on membrane potential (MP) levels and action potential (AP) parameters obtained by us. Materials and methods Neurons from the isolated central neural system (CNS) of the snail Helix pomatia were selected to serve as a model for our experiments. We applied an electrophysiological technique implying recording of intracellular potentials of a neuron. The presented research work is of fundamental nature and reveals some intimate mechanisms of actions made by electromagnetic radiation (EMR) on the cytoplasm membrane of a neuron and its channels that is applicable to cardiomyocytes. An absolutely exclusive distinctive feature of our work is that the specific BBSS EMR used in our experiments has unique characteristics and offers broadband stochastic radiated frequencies in the range from 10n Hz to 10m Hz at an integral radiation power of only 0,1 µW...

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Electromagnetic radiation, cardiomyocyte, neuron, action potential, membrane potential, ion channels, endogenous pacemaker activity

Короткий адрес: https://sciup.org/148308829

IDR: 148308829   |   DOI: 10.12710/cardiometry.2017.11.1727

Список литературы Mechanisms of electromagnetic influences and effects on membrane systems in neurons and cardiomyocytes

  • Kit OI, Shikhlyarova AI, Мaryanovskaya GY, et al. Theory of health: successful translation into the real life. Cardiometry. 2015;7:11-7.
  • Shikhlyarova AI, Мaryanovskaya GY, Barsukova LP, et al. Methodological fundamentals of experimental magneto-therapy of tumors (historical essay). Cardiometry. 2015;7:42-6.
  • Nicholls JG, Marti AR, Wallace BG, Fuchs PA. From Neuron to Brain, 2001. 580 p.
  • Sokolov EN, Arakelov GG, Litvinov EG, et al. Pacemaker potential. Tbilisi, 1975.
  • Sakharov DA. Neurons genealogy. Мoscow: Nauka, 1974.
  • Young NA, Sharma M, Deogaonkar M. Transcranial Magnetic Stimulation for Chronic Pain. Neurosurg Clin N Am. 2014;25:819-32.
  • Pavlopoulos E, Jones S, Kosmidis S, et al. Molecular Mechanism for Age-Related Memory Loss: The Histone-Binding Protein RbAp48. Science Translational Medicine. 28 August 2013;5(200):115.
  • Kandel ER. The Molecular Biology of Memory Storage: a Dialog between Genes and Synapses. Howard Hughes Medical Institute, Columbia University, College of Physicians and Surgeons, New York, USA
  • Chase R. Behavior and Its Neural Control in Gastropod Mollusc. Oxford Univ. Press. New York. 2002. 314 pp.
  • Titlow J, Majeed ZR, Nicholls JG. Intracellular recording, sensory field mapping, and culturing identified neurons in the leech, Hirudo medicinalis. Journal of visualized experiments: JoVE. 2013;81:e50631.
  • Tsechpenakis G, Eugenin J, Nicholls JG. Analysis of nerve activity and optical signals from mouse brain stem to identify cells generating respiratory rhythms. Proceedings -2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro. 2009, article No. 5193289, Pages 1251-1254.
  • Mladinic M, Muller KJ, Nicholls JG. Central nervous system regeneration: From leech to opossum Journal of Physiology. 15 June 2009;587(12):2775-82.
  • Muller KJ, Tsechpenakis G, Homma R. Optical analysis of circuitry for respiratory rhythm in isolated brainstem of foetal mice. Philosophical Transactions of the Royal Society B: Biological Sciences. 12 September 2009;364(1529):2485-91.
  • Blackshaw SE, Nicholls JG. Neurobiology and development of the leech. Journal of Neurobiology. July 1995; 27(3):267-76.
  • Weatherill D, Chase R. Modulation of heart activity during withdrawal reflexes in the snail Helix aspersa. Journal of Comparative Physiology A: Neuroethology, Sensory, Neural, and Behavioral Physiology. April 2005;191(4):355-62.
  • Antkowiak T, Chase R. Sensory innervation of the ovotestis in the snail Helix aspersa. Journal of Experimental Biology. November 2003;206(22):3913-21.
  • Koene JM, Jansen RF, Ter Maat A. A conserved location for the central nervous system control of mating behaviour in gastropod molluscs: Evidence from a terrestrial snail. Journal of Experimental Biology. March 2000;203(6):1071-80.
  • Ratté S, Chase R. Synapse distribution of olfactory interneurons in the procerebrum of the snail Helix aspersa. Journal of Comparative Neurology. 14 February 2000;417(3):366-84.
  • Paternt SU No.1561962 A1. Sposob preparovki central'noj nervnoj sistemy vinogradnoj ulitki. Orlov VI, Laskov VN, Karpenko LD. 1988. Gosudarstvennyj komitet po izobretenijam i otkrytijam pri GKNT SSSR.
  • Orlov V.I., Serdjuk T.S., Suhov A.G. Vnutrikletochnye i vnekletochnye issledovanija roli pejsmekernyh potencialov v organizacii osciljatornoj aktivnosti. In: XVI Mezhdunarodnaja konferencija po nejrokibernetike. 2012
  • Grechenko TN. Pacemaker activity of neurons: genesis and functions. In: A. M. Chernorizov, E. N. Sokolov, V. A. Filippov (eds), Nejron . Tyumen, Tyumen State University Publ., 2008, p. 324 .
  • Orlov VI, Vislobokov AI, Shabanov PD, Marutkina EA. Anesthetic Effective on the External Side of Neurons' Plasmatic Membrane. In: Science in Modern Information Society. Materials of the VII International Scientific Conference. North Charleston, SC, USA, 2015. p. 113-122.
  • Orlov VI, Vislobokov AI, Shabanov PD. In: Clinical Medicine 2014. Materials of the scientific international conference. Ed. by A.I. Vislobokova. 2014. p. 171-182. .
  • Ivlev SA, Sukhov AG, Bondar GG. The membrane puncturing technique at intracellular neuronal activity registration. XI International interdisciplinary congress ‘Neuroscience for Medicine and Psychology'; Sudak, Crimea, Russia; June 2-12, 2015.
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