PCR based detection of insulin like protein from Dolichos lablab L
Автор: Sachin Palekar, Behnaz Patel, Anjali Apte-Deshpande, Anushree Lokur
Журнал: Журнал стресс-физиологии и биохимии @jspb
Статья в выпуске: 2 т.16, 2020 года.
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In the current research work, the genetic region corresponding to both alpha and beta chains of insulin like protein from Dolichos lablab L was identified using PCR. Earlier study by Venâncio et al. , (2003) has revealed that the amino acid sequence of alpha and beta chains of bovine insulin has significant similarity to amino acid sequence of insulin like protein from legume Vigna unguiculata (cowpea). Although the amino acid sequence was available for V. unguiculata , the corresponding DNA sequence was not available in the database. Considering the resemblance in protein sequence, primers were designed using nucleotide sequences of alpha and beta chains of bovine insulin and were used for amplification of these chains from V. unguiculata and D. lablab . Genomic DNA extraction was carried out by the conventional ethanol precipitation method followed by purification using preparative AGE. Standardization on PCR amplification of alpha and Beta chain of Insulin from D. lablab L...
Insulin like protein, bean, pcr based detection, dolichos lablab
Короткий адрес: https://sciup.org/143171170
IDR: 143171170
Список литературы PCR based detection of insulin like protein from Dolichos lablab L
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