The influence of melafen on structural properties of phospholipid membranes
Автор: Alekseeva Olga Mikhaylovna, Krivandin Aleksey Vladimirovich, Shatalova Olga Vladimirovna, Krementsova Anna Vladimirovna, Kim Yuriy Aleksandrovich
Журнал: НБИ технологии @nbi-technologies
Рубрика: Технико-технологические инновации
Статья в выпуске: 5 (14), 2014 года.
Бесплатный доступ
The paper studies the effect of aqueous solutions of melamine salt of phosphonic acid (Melafen), used in crop production, on structural and thermodynamic properties of phospholipid membranes. The multilamellar liposomes formed from individual synthetic dimyristoylphosphatidylcholine (DMPC), or natural phospholipid mixture (egg lecithin) was studied by differential scanning microcalorimetry (DSC) and small-angle X-ray scattering (SAXS). The method of DSC was used to appraise the influence of Melafen aqueous solutions (10-17 M to 10-3 M) on thermodynamic parameters of lipid melting (temperature, enthalpy and cooperation of phase transition) in suspension of liposomes formed from DMPC. On the basis of DSC data it was concluded that the microdomain structural organization of DMPC membranes was altered by Melafen aqueous solutions as polymodal type. We did not reveal by the SAXS method any noticeable integral structural changes in membranes of egg lecithin in multilamellar liposomes as well as in their stacking parameters (period and degree of order) in Melafen aqueous solutions (10-21 M to 10-6 M). This range of Melafen concentrations is wider than that used in crop production. Relying on the results of this work it can be inferred that microdomain structure of membranes composed of individual neutral phospholipids is changed in Melafen aqueous solutions at the wide concentration range of Melafen. At the same time, Melafen does not affect the overall structure of lipid membranes composed of a natural phospholipid mixture.
Melafen, liposomes, differential microcalorimetry, small-angle x-ray scattering
Короткий адрес: https://sciup.org/14968354
IDR: 14968354 | DOI: 10.15688/jvolsu10.2014.5.2
Список литературы The influence of melafen on structural properties of phospholipid membranes
- Albantova A.A., Binyukov V.I., Alekseeva O.M., Mill E.M. The Investigation of Influence of Phenozan, ICHPHAN-10 on the Erythrocytes in vivo by AFM Method. Varfolomeev S.D., Burlakova E.B., Popov A.A., Zaikov G.E., eds. Modern Problems in Biochemical Physics Horizons. New York, Nova Science Publishers, 2012, chapter 5, pp. 45-48.
- Alekseeva O.M. The Influence of Melafen -Plant Growth Regulator, to Some Metabolic Pathways of Animal Cells. Polymers Research Journal, 2013, vol. 7, no. 1, chapter 6, pp. 15-23.
- Alekseeva O.M., Fatkullina L.D., Kim Yu.A., Zaikov G.E. The Melafen Influence on the Erythrocyte's Proteins and Lipids. Herald of Kazan Technological University, 2014, vol. 17, iss. 9, pp. 176-181. (in Russian).
- Alekseeva O.M., Kim Yu.A., Zaikov G.E. Тhe Interactions of Melafen and Ihfans With Animal's Soluble Protein. Herald of Kazan Technological University, 2014, vol. 17, iss. 7, pp. 164-167. (in Russian).
- Alekseeva O.M., Krivandin A.V., Shatalova O.V., Rikov V.A., Fattacfov C-G.G., Burlakova E.B., Konovalov A.I. A Study of Melafen Interaction With Phospholipid Membranes. Reports on Biochemistry and Biophysics, 2009, vol. 427, pp. 218-220.
- Antonov V.F., Smirnova E.Yu., Shevchenko E.V. Lipid Membrane in Phase Transformations. Moscow, Nauka Publ., 1992, p.125.
- Cheremukina G.A., Chernenko S.P., Ivanov A.B., Pashekhonov V.D., Smykov L.P., Zanevsky Yu.V. Automatized One-Dimensional X-Ray Detector. Isotopenpraxis, 1990, vol. 26, pp. 547-549.
- Escriba P.V., Ozaita A., Ribas C., Miralles A., Fodor E., Farkas T., Garcia-Sevilla J.A. Role of Lipid Polymorphism in G Protein-Membrane Interactions: Nonlamellar-Prone Phospholipids and Peripheral Protein Binding to Membranes. Proc. Natl. Acad. Sci. U.S.A., 1997, vol. 94, pp. 11375-11380.
- Fahy E., Subramaniam S., Brown H.A., Glass C.K., Merill A.H., Jr., Murphy R.C., Raetz C.R., Russell D.W., Seyama Y., Shaw W., Shimizu T., Spener F., van Meer G., Van Nieuwenhze M.S., White S., Witztum J.L., Dennis E.A. A Comprehensive Classification System for Lipids. J. Lipid Res., 2005, vol. 46, pp. 839-861.
- Feigin L.A., Svergun D.I. Structure Analysis by Small-Angle X-Ray and Neutron Scattering. New York, Plenum Press, 1987.
- Hampton M.B., Vanags D.M., Porn-Ares M.I., Orrenius S. Involvement of Extracellular Calcium in Phosphatidylserine Exposure During Apoptosis. FEBS Lett., 1996, vol. 399, pp. 277-282.
- Konovalov A.I., Rigkina I.S., Fattachov S-G.G. Supramolecular Structure-Based on Hydrophilic Derivative of Melamine and Bis (Hydroxymethyl) Phosphinic Acid (Melafen) and Surface-Active Substance. Message 1. Structure and Melafen Self-Association in Water and Chloroform. Reports of Academy of Science. Series Chemical, 2008, no. 6, pp. 1207-1214.
- Privalov P.L., Plotnikov V.V. Three Generations of Scanning Microcalorimeters for Liquids. Therm. Acta., 1989, vol. 139, pp. 257-277.
- Shedrin B.M., Feigin L.A. Collimation Correction for Small-Angle X-ray Scattering. The Case of Final Dimensions of Slits. Crystallographia, 1966, vol. 11, pp. 159-163 (in Russian).
- Tharahovsky Y.S. Intellectual Lipid Nano-Containers in Address Delivery of Drug Substances. Moscow, Publishing House LKI, 2011. 280 p.
- Tharahovsky Y.S., Kim Yu.A., Abdrasilov B.S., Muzafarov E.N. Flavonoids: Biochemistry, Biophysicist, Medicine. Synchrobook, Puschino, 2013. 308 p.
- Tharahovsky Y.S., Kuznetsov S.M., Vasilyev N.A., Egorochkin M.A., Kim Yu.A. Taxifolin Interaction With Multilamellar Liposomes From Dimitristoylphosphatidylcholine. Biophysicist, 2008, vol. 53, no. 1, pp. 78-84.
