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Effect of salt stress on expression of carotenoid pathway genes in tomato
Статья научная
Carotenoids, the naturally occurring isoprenoids form essential components of photosynthetic antenna and reaction centre complexes. Thus they play a significant role in absorption, dissipation and transfer of light energy for the process of photosynthesis. The effects of salt stress on carotenoid gene expression in tomato leaves were studied. For that tomato plants were subjected to different concentration of salt water. Morphological characters such as plant height, no. of fruits per plant, chlorophyll content and expression of four major carotenoid pathway genes such as phytoene synthase, phytoene desaturase, zeta carotene desaturase and lycopene beta cyclase were analysed. The quantitative expression analysis using real time PCR has shown a decrease in the expression of all the studied genes as the salt concentration increased. Among the different concentrations of NaCl used for the experiment, it was seen that 200 mM was most detrimental for the carotenoid gene expression. Lycopene beta cyclase, the enzyme that converts lycopene to beta carotene was seen to be highly affected compared to other genes studied showing a 1.87 fold inhibition in its expression at 200 mM NaCl.
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Effect of salt stress on grain reserve composition in ten durum wheat cultivars
Статья научная
Saline soils are defined as those that contain sufficient soluble salts in the root zone to impair plant growth. Wheat is one of the three major cereals dominating world agriculture to date. Durum wheat is the hardest wheat and the concept of durum wheat quality is complex and confusing. Quality factors such as protein content, gluten strength and color have different priorities in various durum wheat markets. Changes in the grain quality of ten durum wheat cultivars under salt stress studied at the greenhouse condition. A factorial experiment was carried out based on completely randomized design with three replications. Treatments were salinity including 0, 60 and 120 mM and ten durum wheat cultivars (Boomer, PGS, 71135, 61130,605, C1351, KND, KDM, Haurani and G1252). Biochemical grain reserves such total protein and carbohydrate content, gluten, phosphorous and beta carotene were measured. Results showed that interaction of salt and cultivars was significant for all studied traits except of beta-carotene. At this experiment, gluten content changed very little with salinity levels. Cultivar 71135 at all salinity levels had the highest gluten content. With increasing of salt severity, the content of protein and carbohydrate decreased and content of phosphorous increased compared with control. In 60 mM salinity, cultivars 61130 and 71135, in 120 mM, 71135 and KDM and in control, 61130 and PGS respectively, had the highest and lowest content of protein. Cultivars 71135 and 61130 had the highest and PGS had the lowest phosphorous content in control. At 60 mM, cultivars 71135 and PGS respectively, had the highest and lowest phosphorous content. At 120mM, 71135 and Haurani respectively, had the highest and lowest phosphorous content. In three salt levels, cultivar 71135 was determined as the most tolerant cultivar according to content of grain reserve composition results.
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Effect of short term NaCl stress on cultivars of S. lycopersicum: a comparative biochemical approach
Статья научная
Tomato is a crop plant with high fruit nutritive value and other useful properties. The cultivation of this species is dependent on many environmental factors, e.g. temperature, salinity, nutrients etc, affecting the yield and reproductive potential of the plant. Salinity in soil or water is of increasing importance to agriculture because it causes stress to crop plants. Plants exposed to an excess amount of salts such as NaCl undergo osmotic stress, water deficit and ionic imbalances and can increase production of reactive oxygen species(ROS). Higher plants possess very efficient enzymatic and non-enzymatic antioxidative defense mechanisms that allow the scavenging of ROS and protection of cellular components from oxidative damage. Studies were conducted to investigate the effect of short term salinity stress on some physiological alterations in three tomato cultivars Pusa Ruby(PR), Punjab Keshari (PK) and Ailsa Craig(AC). Some biochemical parameters (anthocyanin and carotenoeid content, polyamines, proline, cysteine, peroxidase and malondialdehyde) were set and applied at two month old stage of tomato plants. Three tomato cultivars were grown in 0.5xMS for 2 months and at this stage, they were treated with 0 and 200mM NaCl for a short period of six hours in hydroponic conditions. The genotypes exhibited different responses in terms of different osmoprotectant, antioxidant, and pigment level. The relationships among the salinity and accumulation of these compounds in leaf were then determined. It was concluded that, tomato cultivars under study responded differently showing their sensitivity or tolerance to salinity stress. Among three cultivars PK appeared to be more tolerant genotype than the other two cultivars PR and AC. PK could rapidly evolve physiological and antioxidant mechanisms to adapt to salt and manage the oxidative stress. The research was conducted in a completely randomized design with three replications.
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Статья научная
Phytotoxic effects of Pb as Pb(NO 3) 2 and silver nanoparticles on Mung bean ( Vigna radiata ) planted on contaminated soil was assessed in terms of growth, yield, chlorophyll pigments, phenol and flavonoid content at 120 ppm concentration. Experiments were carried out with 4 treatments in 10 days. Treatments were including (T1) control, (T2) silver nanoparticles (50 ppm), (T3) Pb as Pb (NO 3) 2 (120 ppm) and (T4) silver nanoparticles (50 ppm) plus Pb as Pb(NO 3) 2 (120 ppm). Regarding the pigment content, silver nanoparticles-treated plants showed a remarkable increase of chlorophyll. The loss of chlorophyll content was associated with disturbance in photosynthetic capacity which ultimately results in the reduction of Vigna radiate growth. Pb caused a fall in the total content of phenols, while the content of flavonoid not significantly changed. The minimum decrease in root length, weight of root fresh and stem fresh was observed in T4 group, but this factors increased in the other treatments. Also, length of stem and seedling height decreased in control group. Increase length and fresh weight of stem in Pb-treated plants suggest that compatible solutes may contribute to osmotic adjustment at the cellular level and enzyme protection stabilizing the structure of macromolecules and organelles.
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Статья научная
Seeds of ten accessions of carrot ( Daucus carota L.) collected from seven areas in the region of Sidi Bouzid, Lessouda (three accessions: L 1, L 2 and L 3) Faid (one accession: F 4), El Ogla (one accession: E 5), Regueb (two accessions: R 7 and R 8), Lahweze (one accession: L 10) Garet Hdid (one accession: G 11) and Souk Jedid (one accession: S 12) were germinated at 20° C in the dark and in the presence of NaCl (0, 2, 4, 6, 8, 10, 12, 14 and 16 g/l) during 14 days. The results obtained showed that germination is possible until the highest concentration of salt (16 g/l) but germination and speed of germination decrease according to the concentration, so the accession L 3 was the most tolerant and accessions L 1, L 2 and S 12 was the most sensitive.
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Effect of soil salinity on the nodulation and leghaemoglobin in two variables of (Pisum sativum L)
Статья научная
Considerable work has been done on the root nodules and leg-haemoglobin contents of different leguminous plants (Wilson, 1970; Singh et al , 1973; Balsubramanian and Sinha, 1976a, b; Kumar and Garg, 1980; Batra and Bhardwaj, 1981; Gupta and Varshney, 1999). In the present study, an effort has been made to assess the effect of soil salinity on the growth and leg-haemoglobin content of nodules in two varieties of field pea ( Pisum sativum Linn. ). The study was conducted in soil beds (size 1 m2) under natural conditions, in Botanical garden of Gangasheel Mahavidyalaya, Faijullapur, Nawabganj, Bareilly (U.P.). Three replicates were taken for each treatment (control EC 8 CE 8) of both varieties (Var. Pant pea 42 and Pant pea-66). The seeds were sown with gap of equal distance (1 ft.) in soil bed. The result indicates that variety Pant pea-42 is more salt tolerant than Pant pea-66. The number and size of nodules decrease with increase in salinity level in both varieties at all stages of growth (leafy, flowering and maturity). Leg-haemoglobin decreases with the increase level of soil salinity. This study concludes that soil salinity decreases the number of nodules, size of nodules and leg-haemoglobin contents of Field pea ( Pisum sativum Linn. ).
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Effect of some stresses on free proline content during pigeonpea (Cajanas cajan) seed germination
Статья научная
Plants accumulate proline under a variety of stress conditions thereby preventing stress-caused damages. The proline accumulation in germinating seeds of pigeonpea in response to NaCl, Boron and Aluminium treatments were studied. Results showed increased accumulation of proline in salinity stressed germinating seeds. As compare to 100 ppm boron, 10 and 50 ppm of boron treatments promoted proline levels at different stages of seed germination. The content of proline was found to be induced by all aluminium concentrations particularly at 48, 72 and 120 hrs. of seed germination. Whereas reverse pattern of proline accumulation was observed at 96 hrs stage under all studied stresses.
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Статья научная
A pot experiment was conducted to evaluate the beneficial effect of grain presoaking in spermine (0.15 mM), spermidine (0.3 mM) and their interaction on waste water tolerance of wheat plants ( Triticum aestivum L.) variety Sakha 94. In general, waste water caused significant increases in the leaf thickness and ground tissue thickness in flag leaves as well as metaxylem vessel area, xylem vessel area, vascular bundle area in flag leaf and peduncle of main shoot of wheat plants. On the other hand leaf thickness and ground tissue thickness appeared to decrease at concentration 100% of waste water. Irrigation of wheat plants with waste water at all examined concentrations decreased phloem area in flag leaves and peduncle of main shoot of wheat plants. The application of spermine, spermidine or their interaction induced some modifications in the anatomical features of the flag leaf and peduncle of main shoot which appeared to be an adaptive response to heavy metals in applied waste water. Furthermore, Irrigation of wheat plants with waste water stress decreased significantly grain yield / plant. The applied polyamines appeared to alleviate the effect of heavy metals stress in waste water on grain yield. The effect was more pronounced with spermine +spermidine treatment. For anatomical features, the economic yield of wheat plants appeared to be positively correlated with the phloem area but negatively correlated with vascular bundle area and xylem area in flag leaf and peduncle of main shoot.
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Effect of stress hormone antagonists on ovarian follicular development in pre-pubertal rat
Статья научная
Effect of stress on pre-pubertal ovarian follicular development was studied. Fifteen day old female rats were administered under stress (exposed to maternal separation; 6 hours/day) from post-natal day 15 to 21 for 7 days, and appropriate controls were maintained. The time of exposure was randomly changed every day during light phase (7AM to 7 PM) of the day to avoid habituation. There was a significant decrease in serum estrogen levels on post-natal day 21 in stress group rats compared to controls indicating stress response in these rats. However, mean number of healthy follicles in all categories of follicles were significantly lower in stressed rats compared to controls. Concomitant with these changes, mean number of atreitic follicles showed an increase over control values in stressed rats. In contrast administration of Naltrexone (5µg NTX/rat/day), Mifepristone (1 µg MP/rat/day), FSH (10 IU FSH/rat/day) with stressed the significant increases in the relative weight of ovary, uterus, fallopian tube, body weight and the mean number of healthy follicles in the ovary compared to the controls. In the ovary treatment of stressed did not affect primordial follicles. Primordial follicles were reduced in number significantly in the ovary of controls and treated groups when compared with the initial controls whereas there was no significant variation among the controls and the treated groups. The results indicate that stress dose not interfere with the progress of pre-pubertal follicular development. However, it causes increased loss of follicles by atretia.
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Effect of temperature on Triticum aestivum L. seedlings growth and phytohormone balance
Статья научная
He study aimed to determine the effect of short-term heat and cold stresses on growth, accumulation and distribution of abscisic acid (ABA) and indole-3-acetic acid (IAA) in 7 and 14-day-old Triticum aestivum L. seedlings of the frost resistant cultivar Volodarka. A high performance liquid chromatography method has been used to provide a qualitative and quantitative analysis of hormones in leaves and roots. Biometric analysis showed that at the early stage of vegetation (7 days) seedlings were more resistant to temperature stresses, especially to cold, than at the later ones (14 and 21 days), that correlates with the frost resistance of cultivar. The results indicated that at the early stages of growth, after a short-term cold stress (2 h., +2 0C), the amount of free ABA substantially increased in roots, while at the later ones, it occurred after a short-term heat stress (2 h., +40 0C) in leaves. After a heat stress the leaves of 7-day-old seedlings accumulated a conjugated form of IAA. Heat stress caused the accumulation of free IAA in roots of 14-day-old seedlings. Cross stress (cold+heat) caused some increase in the pool of endogenous ABA both in roots and leaves while the amount of free IAA increased only in leaves. Сhanges in the accumulation of the free and conjugated forms of ABA and IAA depended both on the organs and the age of seedlings, and the type of stress and correlated with frost resistance of the cultivar Volodarka.
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Effect of temperature on body temperature and resting metabolic rate in pups of Eothenomys miletus
Статья научная
In order to investigate the ability of ambient temperature and thermoregulation in Eothenomys miletus, body temperature and resting metabolic rate (RMR) were measured during postnatal development (1-49 day) when E. miletus exposed different ambient temperature. The result showed that: body temperature and RMR of pups in E. miletus increased according to the increase of ambient temperature during 1 day to 7 day, showed character of poikilotherms; body temperature of pups were lower in low temperature(5 oC,10 oC), relatively and RMR increased significantly when day age is 14 day, it indicated that the pups showed a certain degree of thermoregulation in this phase. Its thermoregulation ability developed quickly during 7 day to 14 day. RMR of pups was extreme significantly higher in low temperature than that in other temperature when day age was 21 day, it showed that the pups had some thermoregulation to low temperature stimulation. The RMR of pups was showed increasing trend in high temperature(35 oC) when 28 day; on 35 day and 42 day, the thermal neutral zone were 22.5 to 30 oC and approaching its adult level. All of these results indicated that pups of E. miletus in the different growing period had different thermogenesis and energy allocation to maintain stable to body temperature, thermogenesis was weaker in the early phase of postnatal development, most of energy is used to its growth. After pups were weaned, the ability of constant temperature and thermoregulation developed quickly to adjust variations of environment during postnatal development.
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Статья научная
The Fatty acid profiling in Nostoc spongiaeforme and Nostoc calcicola was undertaken at varying growth temperature. The extraction of fatty acid was done by chloroform: methanol method and identification of various SFA, MUFA and PUFA was performed using GC-MS. The result of our study showed that amount of fatty acid content varied with varying temperature. At low temperature, the content of unsaturated fatty acid was maximum in both the Nostoc species but the amount of saturated fatty acid was reduced under the same condition. Whereas, at extreme temperature unsaturated fatty acid was least in both Nostoc species and saturated fatty acid was maximum. Both species showed higher amount of fatty acid content at 20°C and 30°C. Our study concluded that the best temperature for mesophilic cyanobacteria to produce higher amount of fatty acid content is in the range of 20-30°C.
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Статья научная
Liver is involved in several vital functions such as synthesis, secretion, storage and metabolism of plasma proteins and in detoxifying functions. In the present study, hepatocytes were obtained by disaggregation of caprine liver caudate lobe. The cells were cultured under controlled conditions in a CO 2 incubator at different temperatures. The function of hepatocyte culture was evaluated in terms of secretion of albumin, urea and acitivity of Lactate dehydrogenase. Cells were cultured at three different temperatures, i.e. 37, 40 and 42 o C, and at each temperature, the culture was propagated for 24,48 and 72h time interval respectively. At 37 oC, the concentration of albumin and urea increased in the culture temporally where as activity of LDH decreased by 72h. The least square mean (LSM) concentration of albumin, urea in culture decreased and activity of LDH increased significantly (PoC, when compared with respective values at 37 oC. Hence effect of thermal stress(40 and 42 oC) on activity of hepatocyte antioxidant enzymes ( super oxide dismutase, catalase and glutathione peroxidase) and TGF β secretion in vitro was further evaluated. It was observed that there was significant increase (PoC when compared with the enzyme activity at 37 oC, but increase in the secretion of TGF β could be observed only at 42 oC post 48h of incubation. TGF β is associated with apoptosis and cytotoxicity. It was observed that viability of the cells decreased significantly (PoC post 48h of incubation. It can be concluded that hyperthermic conditions were observed to be stressful for hepatocytes during in vitro conditions, which proved to be more significant at 42 oC post 48h of incubation. Further work has to be carried out with some supplements which can reduce the hyperthermic effect. In vivo studies in caprine species will give further insight on thermal stress. To the best of our knowledge no studies on hyperthermic effect on hepatocytes in caprine species in vitro or in vivo has been reported.
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Effect of thiourea on yield and quality of potato ( Solanum tuberosum L.)
Статья научная
A field experiment was carried out to study the effect of five concentrations (0, 250, 500, 750 and 1000 mM) of thiourea application on mother tubers on yield and quality attributes of harvested potato, variety Spunta in the region of Chott-Mariem in Tunisia. Treated tubers (Ø = 50 mm) were planted in field after breaking dormancy at a spacing of 80 cm x 30 cm according to completely randomized design (CRD) in three replications. Quality attributes of potato tubers especially fresh matter and dry matter of plant, total reducing sugars in leaves, tuber yield, number and diameter of tubers per plant and number of sprouts per tuber was measured. Soluble proteins and starch content in potato tubers were also quantified. In general we noted that application of thiourea showed significant influence on yield and on quality of tubers comparing to control. Maximum tuber yield per plant, maximum number of tubers per plant and maximum starch content was recorded with 250 mM of thiourea. Moreover, highest dry matter of potato plant was found also at this concentration. While increasing dose of thiourea thereafter it showed slight significant improvement and do not affect significantly the diameter of tubers comparing to control.
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Effect of various doses of Cannabis extract on male reproductive system of FBV/N strain albino mice
Статья научная
The experiment focused on the endocrinological, histopathological and biochemical effects of a Cannabis extract (CAN) containing cannabinoids from Cannabis sativa on the male reproductive system of mammals. The experiment involved five groups of albino mice (FBV/N strain), with 12 mice in each group. The groups were labeled as follows: CV-I, a control group treated with alcohol vehicle; CAN-II, a group treated with 40mg/mouse of CAN; CAN-III, a group treated with 60mg/mouse of CAN; CAN-IV, a group treated with 80mg/mouse of CAN; and CAN-V-R, a withdrawal group allowing for a 45-day recovery period after receiving 80mg/mouse of CAN treatment. The experimental subjects were given CAN through intraperitoneal injection. At low doses (40mg/mouse and 60mg/mouse), significant histopathological changes were observed, including a reduction in the weight of the testes, seminal vesicle, and epididymis, shrinkage of seminiferous tubules, impairment of the spermatogenic cycle, and a reduction in sperm count. There were also significant changes in protein, lipid, and cholesterol content, as well as in the concentration of steroidogenic enzymes such as 3-β hydroxysteroid dehydrogenase (3β-HSD), 17-β hydroxysteroid dehydrogenase (17β-HSD), testicular acetylcholinesterase (tACHE), and testicular angiotensin-converting enzyme (tACE) at low doses. The reduction of 3β-HSD and 17β-HSD at low doses also affected the concentration of serum testosterone, supporting the higher value of cholesterol content. Additionally, a reduction in gonadotropins (FSH and LH) at low doses was documented, along with hypertrophied pituitary due to positive feedback stimulation. The reduction of lipid content was likely related to lipid peroxidation due to an increase in the amount of conjugated dienes. However, in the high-dose group (80mg/mouse) and the withdrawal group (CV-V-R), gradual recovery in all parameters was observed. In conclusion, it is suggested that high-dose CAN treatment produces a sort of stress that likely activates some stress-induced genes responsible for minimizing the drastic effects of CAN produced by low-dose treatment.
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Статья научная
Objectives: To study the effects of various treatments on seed germination of Dolichandrone falcata (Wall. Ex DC.) Seem. by using different substrates; and also, to study the accumulation of proline as a stress response to the given treatments.
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Статья научная
Important field crops grow less successfully contaminated by weeds. The environment and human health have suffered severely from improper weed control methods and the unnecessary and inappropriate application of chemical fertilizers, and caused an increase in weed variety resistance. The phytochemicals found in allelopathic plants have gained importance as potential biological equivalents for synthetic herbicides. The enzyme activity of Lablab purpureus (L.) sweet and Vigna unguiculata (L.) Walp can be impacted allopathically by weed extracts. In Vigna unguiculata (L.) Walp and Lablab purpureus (L.) sweet, the enzyme activities of Phyllanthus niruri (L.) and Chloris barbata Sw. weed extracts treated as foliar sprays were assessed throughout the pre-flowering, flowering, and post-flowering stages. This work shows that an aqueous weed extract of Lablab purpureus (L.) sweet and Phyllanthus niruri (L.) and Chloris barbata Sw. can enhance enzyme activity in Vigna unguiculata (L.) Walp, hence providing the possibilities for the development of plant growth.
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Статья научная
In Mali, citrus fruits play an important part in both human nutrition and the country's economy. They represent an important source of income for citrus growers. Citrus production in Mali is highly diversified, but the current trend is towards the Tangelo Orlando or Citrus×tangelo variety. The aim of this study was to determine the effects of two phytohormones (2,4,5-T acid and AG3) and a mineral salt (KNO3) on the production of Tangelo Orlando. The experiment was carried out in Koulikoro district, especially on the experimental plot of the IPR/IFRA of Katibougou. The plant material used was white-pulp Tangelo Orlando. Two phytophytohormones (2,4,5-T acid, and AG3), a mineral salt (KNO3) and distilled water (control) were used to treat the young plants. The experimental design was a Fischer block with three replications. Observations were made on the parameters of development. The results showed significant differences (p
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Статья научная
The present study investigated the biochemical impact of ZnO nanoparticles (NPs) and salinity stress in Camelina (Camelina sativa L.) plants. ZnO NPs were applied at 0, 20, 40 and 80 mgL-1 and salinity stress (NaCl) were at 0, 50, and 100 mM. Salinity stress significantly enhanced total phenolic compounds, anthocyanin, carotenoid and ability to scavenge the DPPH radical in shoots and roots. We demonstrate that the ZnO NPs, plant carotenoid content is profoundly affected by salinity and ZnO NPs. Salinity stress decreased zinc concentration in shoots and root, but increased macro-element such as calcium (Ca) and phosphorous (P). Application of 20 mgL-1 ZnO NPs gave the most impact contents of calcium (Ca), phosphorous (P). With increasing ZnO NPs concentration, zinc (Zn) content was increased. Foliar application of ZnO NPs did not show any significant effect on flavonoid content in Camelina but caused increases in DPPH radical scavenging capacity in shoot and root. We found that the amount of phenolic compounds in shoot was decreased at 20 mgL-1 of ZnO NPs concentration. ZnO NPs at 20 mgL-1 was more provocative to reduce the effects of NaCl. Therefore, the results suggest that the appropriate concentration of ZnO NPs (20 mgL-1) could overcome the negative effects of salt stress in Camelina.
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Статья научная
Background: The present study investigated the effects of four days of fasting on colonic temperature (CT) and body weight responses in 20 adult male Rouen ducks (Anas platyrhynchos domesticus) during the hot-dry (12:12 light/dark cycles) and cold-dry (harmattan) (11:13 light/dark cycles) seasons under natural light/day cycles in tropical conditions. The ambient temperature and relative humidity of the pen and the CT of the ducks were recorded bihourly for four days before fasting and subsequently for another four days during fasting. During the fasting period food was completely withdrawn from the ducks at 06:00 h for four days (96 h), but the ducks were given access to clean drinking water ad libitum throughout the fasting period. Results: A major finding in the present study was that four days of food deprivation, photoperiods, season and duration of fasting did not induce hypothermia or changes in circadian rhythmic pattern of CT in the Rouen ducks. The result, for the first time, suggests that circadian modulation of fasting-induced hypothermia was lacking in Rouen ducks. The mesor and amplitudes of CT obtained during both the harmattan and hot-dry seasons before fasting were not different (P > 0.05) from those obtained during fasting. The acrophases in both seasons before and during fasting were restricted to the photophase at 14:00 h, except for the acrophase in fasted ducks during the hot-dry season, which was delayed to 16:00 h. Body weight of the ducks decrease significantly (P > 0.05) only on the 4th day of fasting. Conclusion: Overall, unlike other birds and mammals, the Rouen ducks showed greater starvation resilience, apparently due to an unknown component of fasting resistance or regional heterothermy. Future studies to elucidate the mechanism by which Rouen ducks were resistant to fasting are still required.
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