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Статья научная
The present study investigates the role of 24-epibrassinolide (EBL) in inducing plant tolerance to salinity. Seedlings of pepper (Capsicum annuum L.) were grown in the presence of 70 mM NaCl and were sprayed with 10-6 M EBL at 7 days after transplantation and were sampled at 28 day. The plants exposed to NaCl exhibited a significant decline in relative growth rate, net CO2 assimilation, stomatal conductance, transpiration and water use efficiency. However, the follow up treatment with EBL significantly improved the above parameters. EBL treated plants had greater relative growth rate compared to untreated plants when exposed to salt stress. Application of EBL increased photosynthesis by increasing stomatal conductance in both control and salt stressed plants and may have contributed to the enhanced growth. The water use efficiency was improved because CO2 assimilation is more important than the transpiration.
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Статья научная
Among various environmental stresses, salt stress is extensively damaging to crops production in many areas of the world. The enhancement of plant tolerance is important for plant productivity. Brassinosteroids plays an important role in the plant response to adverse environmental conditions such as salt stress. Therefore, an experiment was conducted to explore the role of exogenously applied 24-epibrassinolide on flower and fruit production of pepper in presence or absence of NaCl-stress (70 mM). The plants were sprayed with 10-6M of 24-epibrassinolide at vegetative, buds formation and early fruiting. 24-epibrassinolide improves flower number, fruit number and yield per plant, but was without effect on fruit mass and size. The detailed measurement of these parameters indicates that the effects of 24-epibrassinolide depend on the development stage of pepper plant and the application frequency of this hormone.
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Статья научная
The present work was conducted to study the effects of 24-EBL on photosynthetic pigments (total chlorophyll, chl a, chl b, carotenoid, anthocyanin and flavonoid content) and activities of antioxidative enzymes (guaiacol peroxidase, catalase, superoxide dismutase, ascorbate peroxidase, glutathione reductase, dehydroascorbate reductase, mono-dehydroascorbate reductase, polyphenol oxidase, glutathione peroxidase and glutathione-S- transferase and protein content) in 7-day old seedlings of Raphanus sativus exposed to cadmium and mercury toxicity. Findings of present study were revealed that brassinolide was proved beneficial for amelioration of Cd and Hg stress by altering various metabolic processes of plant.
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Статья научная
The aim of present study was to explore the ameliorative impact of 28-homoBL on morpho-physiological attributes, photosynthetic pigments and sugars of Brassica juncea L. exposed to oxidative stress caused by extreme temperatures (4 and 44 °C). For this, experiments were carried out at the Plant Physiology Laboratory, Department of Botany, Punjabi University, Patiala. Effect of different degrees of temperature (4 and 44 °C) taking 24 °C as control was studied. 28-homoBL (10 -6, 10 -9 and 10 -12M) primed and unprimed seeds of B. Juncea L. in terms of antenna complexes and end products of photosynthesis that is total carbohydrates and total soluble sugars was investigated. All concentrations of 28-homoBL used in present study showed different effects on morphology and light quenching pigments. All concentrations of 28-homoBL showed promoting effect on growth and light quenching pigments. The carbon makeup ameliorated positively in stressed and non-stressed components of photosynthetic machinery and 10 -9 M 28-homoBL showed best results. In conclusion 28-homoBL showed great potential in protecting the reaction centre of photosynthetic machinery from oxidative stress caused by extreme low and high temperatures but in very dose dependent manner and thus modulate the carbon skeleton of the plant.
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Статья научная
High temperature is a serious threat to crop production. Brassinosteroids (BRs), a group of plant steroidal hormones, can reduce effects of abiotic stresses. The present study was aimed to study the potency of brassinosteroids on high temperature induced changes in Indian mustard ( Brassica juncea L.) for effects on growth, chlorophyll, photosynthesis, photosystem II, antioxidant system and proline. Surface sterilized seeds of Indian mustard were sown in pots, grown for 21 days and treated with double distilled water or 0.01 µM of 28-homobrassinolide. Treated plants, after 24 h, were exposed to 30°C or 40°C for 48 h. One set of plants were kept at ambient temperature, 25°C, as the control. Plants were harvested at 30 days stage of growth to assess the various parameters. Plants exposed to 40°C had a decline in growth, leaf water potential, chlorophyll, photosynthetic rate, and activities of carbonic anhydrase (E.C.4.2.1.1) and nitrate reductase (E.C.1.6.1.1). The 28-homobrassinolide alone improved growth and photosynthesis responses along with various enzymes activities. Treatment of plants with HBL prior to exposure to 40°C, partially reduced damage and completely controlled damage when exposure was to 30°C. Levels of the antioxidative enzymes catalase (E.C.1.11.1.6), peroxidase (E.C.1.11.1.7), and superoxide dismutase (E.C.1.15.1.1), and the level of proline increased in response to 30 or 40°C and were further enhanced in the presence of 28-homobrassinolide. Plants grown under high temperature had increased levels of H 2O 2; application of HBL before temperature treatment decreased H 2O 2 content compared to the control. Elevated levels of antioxidative enzymes and proline might be responsible for conferring tolerance to high temperature stress in Indian mustard and overcome the loss of productivity of the crop.
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Статья научная
Background: The standardization of herbal medicines is an essential step in evaluating the quality of medicines based on drug concentration, physical and chemical standards. Due to the fact that the plant of Senna auriculata L. has a great medicinal potential with information about the compounds present in the various parts of the plant, it was considered important to study the preliminary physicochemical properties. Results: Samples A & B were collected from natural habitats and Samples C & D were collected from polluted areas. Physicochemical properties of powdered plant material of S. auriculata include extractive value, ash value, loss on drying, and fluorescence behaviour were determined as per standard methods. The water-soluble extractive value in the leaves and flowers of samples A and B was greater than samples C and D. In terms of alcohol soluble extractive value, both samples A and B had a high percentage yield in their leaves and flowers, whereas samples C and D had a somewhat lower percentage yield. The leaves and flowers of samples A and B had lower moisture content percentages, whereas samples C and D had somewhat higher moisture content percentages. Conclusions: The results of the physicochemical analysis suggest that samples A and B were far preferable to samples C and D in terms of quality. According to the findings of this study, collecting medicinal plants from their natural habitat is preferred since contaminated environments might impact the physicochemical properties of the plant material.
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Статья научная
Lichens are group of nonvascular plants. Lichen absorbs water and nutrient passively from their environment. Because of this lichens are particularly sensitive to environmental factors such as temperature, water availability, and air pollutants and/or by substances accumulates in lichen from the environment through variety of mechanisms including particulate trapping, ion exchange, extracellular electrolyte adsorption, hydrolysis and intra cellular uptake. Air pollution control is more complex than most other environmental changes. A study has been done to evaluate the air pollution status by means of lichen through the biochemical variability of three macro environment (semiurban area Arambagh, urban area Burdwan and industrial area Durgapur). The study results revealed that there exist inverse relationship between pollutant load and pigment content (chlorophyll and carotenoid content). Average concentration of chlorophyll 'a', 'b', and total chlorophyll was found in following order, Site-II>Site-III>Site-I. From the result of Chlorophyll Stability Index (CSI) it has been found that the chlorophyll degradation is highest in Site-II followed by Site-III and lowest in Site-I. There is a strong inverse relationship of chlorophyll content in lichen with SO2and NOxbut the level of SPM found highest in Site-I followed by Site-III and lowest in Site-II. Moreover, biochemical constituents (protein, sugar, phenol and proline) of all the representative samples were also vary from site to site. The study results reveal that the amount of soluble sugar was highest recorded at Site-III followed by Site-I and lowest in Site-II. Again correlation study indicates that there is positive relationship between soluble sugar and phenol content at 5% level of significance. Therefore, it also indicates that the lichen metabolite and other physiological action monitored by SO2, NOx SPM level in the air. Studies showed that the semiurban area was under more vulnerable condition than industrial and urban area of Hooghly and Burdwan district. Moreover the micrograph study of representative lichen samples directly proved that lichen structure affected by air pollutants, but the extent of deformation exclusively depends on the level of pollutants present in the air. Results also suggest that different level of air pollutants (SO2, NOxand SPM) affect the biochemical parameters of lichen, but more extensive study is needed to confirm the relationship between air pollution status and lichen biochemistry.
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Статья научная
Background : Cascabela thevetia (L.) Lippold, a popular member of family Apocynaceae, (Yellow Oleander), being one of the preferred weapons for suicides in villages of India is grown in garden and roadside ways as a tree a. Pharmacologically active constituents include terpenoids, a-vonoid, steroids and glycosides in seeds.
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A modified D-roms method for determination of lipid peroxidation
Статья научная
The need for simple and low costing and less time consuming methods for the determination of lipid peroxides and oxidative stress is urgent because oxidative stress is still unable to become a routine laboratory biomarker due to the sophisticated procedures and costing, a matter that made oxidative stress a research only biomarker, despite its crucial value as a risk factor and pathologic status, for many inflammatory and metabolic diseases. This work aimed at developing a modified rapid method to determine serum lipid peroxides at wavelength 492 nm and reference wavelength 630 nm. The results showed linear responses with increasing concentration and that the cheap N,N-DM-P-Phenylenediamine diHCl can be used as a chromogen under saving conditions of pH, temperatures and volume of sample.
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A study on serum electrolyte pattern during ambient stress in Murrah buffalo of arid tracts in India
Статья научная
A study was undertaken to assess the effect of ambient stress on serum electrolyte pattern of Murrah buffaloes of arid tracts in India. Healthy adult female Murrah buffaloes were grouped according to physiological states and screened during moderate, hot and cold ambiences. The mean values (m mol L-1) of serum inorganic phosphorus, calcium and magnesium during moderate ambience were 1.80± 0.01, 2.80± 0.01 and 0.90± 0.002, respectively. During hot and cold ambiences significant (p≤0.05) decrease was observed in the mean value of each electrolyte. Decline was higher during hot ambience as compared to cold ambience. In each ambience it was noticed that serum electrolyte concentration of pregnant dry animals was lowest significantly (p≤0.05) as compared to non pregnant milch followed by pregnant milch. Multipara animals showed significantly (p≤0.05) lower value of each electrolyte as compared to primipara. It was concluded that ambient stress during hot and cold ambiences resulted in depletion of electrolyte levels of the buffaloes of all physiological states.
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Статья научная
The ability of plants to tolerate drought conditions is crucial for plant survival and crop production worldwide. The present data confirm previous findings reported existence of a strong relation between abscisic acid (ABA) content and amino acid accumulation as response water stress which is one of the most important defense mechanism activated during water stress in many plant species. Therefore, free amino acids were measured to determine any changes in the metabolite pool in relation to ABA content. The ABA defective mutants of Arabidopsis plants were subjected to leaf dehydration for Arabidopsis on Whatman 3 mm filter paper at room temperature while, tomato mutant plants were subjected to drought stresses for tomato plants by withholding water. To understand the signal transduction mechanisms underlying osmotic stress-regulating gene induction and activation of osmoprotectant free amino acid synthesizing genes, we carried out a genetic screen to isolate Arabidopsis mutants defective in ABA biosynthesis under drought stress conditions. The present results revealed an accumulation of specific free amino acid in water stressed tissues in which majority of free amino acids are increased especially those playing an osmoprotectant role such as proline and glycine. Drought stress related Amino acids contents are significantly reduced in the mutants under water stress condition while they are increased significantly in the wild types plants. The exhibited higher accumulation of other amino acids under stressed condition in the mutant plants suggest that, their expressions are regulated in an ABA independent pathways. In addition, free amino acids content changes during water stress condition suggest their contribution in drought toleration as common compatible osmolytes.
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Статья обзорная
Andrographis paniculata is an annual herbaceous plant of the family Acanthaceae. Andrographolide (a bicycle diterpene) is the main component of A. paniculata and responsible for its bitter taste. Abiotic stress considerably affects the life cycle of plants through morphological, biochemical, and physiological changes. These stresses also act as elicitors for the synthesis of secondary metabolites (SMs) in plants. Secondary metabolites help plants to survive in adverse conditions via alleviating osmotic stress and reducing Reactive Oxygen Species (ROS). In this review, we discuss the response of the antioxidative system and biosynthesis of secondary metabolites in A. paniculata under abiotic stress. The mechanism involve in biosynthesis of ent-labdane diterpenoids is also discussed.
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Absorption of power plants СО 2 emissions by coniferous tree stands
Статья научная
The article reviews the ability of coniferous (common pine, siberian larch and siberian spruce) stands growing in 9 municipal districts of the Irkutsk region to absorb СО 2 technogenic emission of heat power plants. (EIGAF) index is suggested to characterize gas-absorbing (СО 2-absorbing) activity; the index reflects proportion between СО 2 technogenic emission and photosynthetic productivity (GPP) of coniferous tree stands. СО 2-absorbing capacity in 8 of the monitored districts has been shown to significantly exceed the amount of carbon dioxide emission from heat power sector. The index values EIGAF=0.01-0.97 demonstrate that СО 2 technogenic emission amounts to 1-97% of coniferous stands photosynthetic productivity in the areas under study. At the same time, the most industrially developed Angarsk district shows СО 2 photosynthetic absorption to be 8-12 times lower than technogenic СО 2 emission. Reasons of low gas-absorbing capacity of coniferous tree stands of this area are discussed.
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Abuse of anabolic androgenic steroids
Статья научная
According to the International Olympic Committee, the abuse of anabolic androgenic steroids (AASS) is found in over 50% of positive doping tests. AASS abuse is not restricted to the organized sports and widespread use. It remains as an unsolved public-health problem. Lower black market price, easier access to AASS, bodybuilding clubs and internet advertising are factors of this increasingly misuse. There is not real data about the prevalence of AASS abuse in various populations or countries, because most of athletes or students, due to their prohibition or ethical aspects do not admit to AASS abuse. Often they are aware of the risks of their choice and yet, are eager to put themselves at risk without deeper consideration. The abusers use them to improve their physical fitness and appearance. Present article has been collected to elucidate the risks and adverse effects of AASS and explanation of mechanisms of these events.
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Accumulation of heavy metals in soil-crop systems
Статья обзорная
The health concerns associated with heavy metal pollution (HMP) in agricultural soils have garnered attention on a global scale, and evaluations of the dangers to human health are based on research on the buildup of heavy metals in soil-plant systems. The pedosphere, hydrosphere, atmosphere, lithosphere, and biosphere all suffer from environmental pollution. As a result of the industrialization of many nations, heavy metal pollution is one of the most important environmental problems today. Heavy metal pollutants in the environment have been removed using a variety of ways, however these procedures have drawbacks such high cost, lengthy process times, logistical issues, and mechanical complexity. To use phytoremediation, which uses plants to remove, transport, and stabilize heavy metals from soil and water, one must understand the accumulation of heavy metals in plants and the function of plants in eliminating contaminants. Physiological and metabolic processes that enable plants to phytoremediator heavy metal-contaminated locations are now being optimized via genetic engineering techniques. This review investigated the accumulation of seven common heavy metals in soil agriculture systems: Cd, Cr, As, Pb, Hg, Cu, and Zn. It is reported that wheat was generally more prone than corn to acquire heavy metals. The seven heavy metals were ranked as follows, in ascending order of accumulation in grains: Pb
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Статья научная
Drought is one of the environmental stressors that limits plant growth, production, and is a great threat to the rising population and security throughout the globe. In the present work, we aimed to check the impact of drought stress given at different days interval drought (DID) on proso millet and its tolerance capability. The pot culture experiment was conducted and drought stress was given at 3 DID, 5 DID, and 7 DID respectively, and sampling was done on 15, 30, and 60 DAS, and unstressed plants were irrigated regularly. Drought stress results in decreased protein contents in both the roots and shoots of P. miliaceum on all sampling days. However, with increasing drought intensity and duration there was a tremendous increase in amino acids, and proline contents as well. Therefore, increasing osmolytes like proline with an increased drought period proves the role of proline in stress tolerance of proso millet and helps it to survive by maintaining osmotic potential, water influx, and detoxifying ROS. Thus, proso millet can be cultivated in drought-prone areas, and used for the breeding program.
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Статья научная
Accumulation pattern of Al, Cd, Fe, Hg, Cr, Cu, Pb, Ni and Zn in Chromolaena odorata plants grown in Hoagland nutrient solution and soil contaminated with known quantities of the above said metals was investigated. Significant variations in the quantity of accumulation as well as distribution among plant parts like root, stem and leaf were shown between the metals. Accumulation of Pb was maximum in the root followed by Fe and Al. Maximum quantity of each metal was accumulated in the root as compared to stem and leaf. Drastic differences in the accumulation pattern of metals between the nutrient solution and soil culture was observed. Comparatively small quantity of metal was accumulated in the plants of soil despite several fold quantity of each metal was given. The results are discussed in terms of BCF, TF, metal specificity as well as detoxification mechanisms.
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Статья научная
The Indian apple snail Pila globosa (Swainson) experiences an annual cycle of aestivation (summer sleep) as a survival strategy during a hot and dry period to avoid damage from high temperatures and the risk of desiccation. Alterations in the external environment reflect in their haemolymph. Changes in enzyme levels because of any type of stress are immediately reflected in the functional responses of animals. These environmental factors can be simulated in the laboratory so as to study enzymatic alterations occurring in the haemolymph of snails to overcome the adverse features during aestivation. Phosphatase enzymes are important for many biological functions. Acid and alkaline phosphatase activities have been studied in the haemolymph of three months aestivated and active snails. The activity level of enzymes ACP decreased and ALP increased significantly in the haemolymph of aestivated P. globosa when compared to active snails. The significance of these findings is discussed. This investigation explains the adaptability of P. globosa to overcome the adverse features during aestivation. This study also reveals that Pila globosa is a suitable model for studies on aestivation and provides an interesting case of aestivation.
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Activation of cell death in the sugar cane suspension culture by the exposure to high temperature
Статья научная
The process of cell death in a sugar cane suspension culture after exposure to high temperature (45, 50, 55 and 60 °C) during 10 min has been studied. It has been revealed that treatment of cell culture at 50 °C did not cause an immediate cell death, but 50% of the cells were dying for the next 48 h. Exposure of cell culture to more high temperature (55 - 60 °C) caused a massive cell death occurred instantly after treatment. The development of cell death after the treatment at 50 °C was accompanied by the protoplast condensation, increased generation of reactive oxygen species and hyperpolarization of the mitochondrial inner membrane. Obtained results indicate on the active character of the cell death process, induced by the moderate heat shock in sugar cane suspension culture.
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