Статьи журнала - Журнал стресс-физиологии и биохимии
Все статьи: 941
Ca2+ induced lipid peroxidation accentuates aging in rat brain mitochondria
Статья научная
Aging in rats is multifactorial and include decline in biological, physiological, psychological and social changes. The antioxidant imbalance is one of the commonest factors during aging and age-related diseases. Several studies have identified the participation of various antioxidant’s differential activity levels in the brain. However, Ca2+ is known to alter the homeostatic balance of these antioxidants. Lipid peroxidation is the chief marker of membrane architecture disruption in the aging and neurodegenerative diseases. In the current study, we have focused on the contribution of Ca2+ participation, its concentration and effect on the lipid peroxidation at various stages of rat’s life. We have observed that LPO induction is increased in mitochondria isolated from aged rats in comparison to young adult and neonatal rats. Treatment of 100 to 300uM Ca2+ has shown a steady increase in lipid peroxidation of up to 20% in mitochondria isolated from aged rats while neonates and young rats have displayed differential levels of peroxidation from 17% to 15%. Thus, alleviating role of Ca2+ in lipid peroxidation in aging and neurodegeneration.
Бесплатно
Статья научная
Fourier transform infrared spectroscopy (FTIR) analysis has been employed to identify various functional groups involved in cadmium biosorption into Ulva lactuca (Chlorophyta) and Padina pavonica (Phaeophyta) seaweeds exposed to different cadmium (Cd) (0, 2.5, 5 and 10 mg/L) concentrations for 4 days. Data revealed that Cd stress induced chemical structure alteration into studied seaweeds. Overall, for U. lactuca, carboxylic acids (C-O) & amides (C-N stretch) groups and aromatics groups (C-C stretch) showed a shift in wavelength with Cd stress referring that they may be play an important role in Cd biosorption process. Whereas, for P. pavonica, amides groups (C=O stretch), alcohols (O-H stretch) and phenols (H-bonded) groups showed a slight shift in wavelength indicating that these peaks could involve in Cd biosorption process. Thereby, FTIR analysis seems to be a potent tool for screening chemical structure alteration induced by Cd stress in marine ecosystems.
Бесплатно
Centella asiatica: a concise drug review with probable clinical uses
Статья обзорная
Centella asiatica (Gotu kola) is an imperative herb in Ayurvedic medicine, often mentioned in combination with the related European marsh pennywort (Hydrocotyle vulgaris). About 20 species recounted to Gotu kola cultivate in most parts of the tropic or wet pantropical areas such as rice paddies and also in rocky and higher elevations. Centella asiatica (Gotu kola) is known as longevity herb and used widely in India and Nepal as part of the traditional Ayurvedic medicine. In Samskrita, it is called 'Mandūkaparnī' as its leaf appears as a standing frog from its backside. It is also called 'Brahmi the goddess of supreme wisdom and 'Saraswati the goddess knowledge & wisdom. Its roots and leaves are used for medicinal purposes and provide important health benefits related to healthy veins and blood vessels, to treat skin disorders, help with better memory and improve brain function.
Бесплатно
Cerebral Microcirculation during Respiratory Arrest in Deep Experimental Rat Hypothermia
Статья научная
We investigated the effect of breath stimulation on cerebral microcirculation at deep hypothermic condition of Wistar rats using the method for measuring microcirculation in real time and optical system of LUMAM-1 microscope. After a hypothermic respiratory arrest in the group without the usage of mechanical ventilation, the blood flow velocity decreases sharply and completely stops after 10 minutes. In the group with the usage of mechanical ventilation, the blood flow velocity is increased compared to the rate when breathing stops and for a long time stays at an elevated level. We have found that mechanical lung ventilation significantly improved cerebral blood flow and prolonged heart function.
Бесплатно
Статья научная
In nature, plants are always subjected to various abiotic stresses such as drought, salinity, high temperatures and so on. Among these drought is a worldwide problem, responsible for limiting the growth, physiology and productivity of plants, thus has become a challenge for global food security towards growing population. Proso millet ( Panicum miliaceum L.) belonging to family Poaceae grows under wide environmental conditions and different soil profile. In this view, an experiment was conducted to investigate the range of tolerance and change of metabolic activities of Proso millet under drought stress. The control plants were irrigated regularly and treated plants were irrigated at 3, 5, 7, day intervals up to 60day interval drought (DAS). The root and leaf samples were collected on 30 DAS, 45 DAS and 60 DAS respectively for morphological and biochemical analysis. It was found that with increasing duration of water deficit, tremendous increases of antioxidants activities were recorded at all growth stages compared to control on 7DID at 60DAS. Furthermore, a decreased rate of growth, biomass and chlorophyll content was recorded in treated plants than control. Therefore, it can be concluded that proso millet has affinity to survive under prolonged drought stress and can help to understand the mechanism of photosynthetic efficacy for improving crop productivity.
Бесплатно
Статья научная
In order to study the influence of expression of heterologous genes of different origin ( cyp 11A1 and des C) on canola thermotolerance improvement on leaf membrane level the fatty acid composition was analyzed under short-time heat test. C yp 11A1 gene encodes cytochrome P450 SCC from bovine adrenal cortex mitochondria and was shown to affect the biosynthesis of steroid compounds. DesC gene encodes ∆9-acyl-lipid desaturase of cyanobacterium Synechococcus vulcanus. Decrease in palmitlinolenic acid content and index unsaturation as well as increase in total fatty acid and palmitic acid content were identified in cyp 11A1 canola in comparison with wild-type plants in stressfull conditions. But control and des C plants demonstrated similar changes in saturated (16:0), trienoic (16:3 and 18:3) fatty acid quantity, total fatty acid content and index unsaturation. Heterologous des C gene expression did not influence fatty acid composition and did not give advantages for plant under heat. Integration of cyp 11A1 gene in canola led to thermotolerance improvement on membrane level.
Бесплатно
Статья научная
In the present study, the effects of temperature changes during summer and winter seasons on haematological parameters, total cholesterol, and cortisol level in male and female Syrian Awassi sheep were studied. Blood samples were collected weekly from ten animals (five rams and five ewes) during August and January months (2022-2023), and these samples were analyzed by a Veterinary Hematology Analyzer to determine the haematological parameters, and by enzyme-linked immunosorbent assay technique (ELISA) to determine the level of cortisol and total cholesterol. The results showed that there were significant differences in white blood cells (WBC), monocytes (MON) and granulocytes (GRA), as well as in haemoglobin (HGB) between August and January for both females and males. No significant difference was recorded between the two months for both sexes regarding platelet count (PLT) and mean platelet volume (MPV). There were significant differences (P 0.05) was recorded for males between these two months. It was concluded that there were significant differences in the values of some blood components between summer and winter for males and females Awassi sheep, but the differences in the level of total cholesterol and cortisol were clearer in the females than in males between summer and winter. In general, this study showed the ability of the Syrian Awassi sheep to adapt higher temperature degrees than the lower ones in semi-arid area where Syrian Awassi flocks are raised.
Бесплатно
Changes in jasmonic and salicylic acids levels in barley challenged with cochliobolus sativus
Статья научная
Spot blotch (SB), caused by Cochliobolus sativus is a common foliar disease of barley worldwide. The phytohormones jasmonic acid (JA) and salicylic acid (SA) play important functions in plant defense systems. However, the changes in JA and SA pathways in response to SB disease have been poorly documented. In the current work and to better understand the mechanisms of barley resistance to this disease, JA and SA pathways were evaluated in resistant ‘Banteng’ and susceptible ‘WI2291’ cultivars across four time points post pathogen challenge. The data showed that JA and SA contents were elevated in fungus-inoculated and non-inoculated leaves in both resistant and susceptible interactions 24h post inoculation. However, although JA signaling was activated in parallel with SA signaling up to 72h in both cultivars, JA had no significant differences across four time points as compared with non-inoculated controls. Furthermore, the resistant cultivar ‘Banteng’ constitutively contained higher levels of SA (956.2 ng/g) comparing with the susceptible one ‘WI2291’ (192.5 ng/g) 96h post inoculation, whereas, JA pathway was weakly activated over time. We thus hypothesized that SA signaling has an important function in resistance mechanisms against SB disease, whereas JA signaling has a contrasting role in this defense system, which might prove crucial information concerning barley signaling events induced by C. sativus invasion.
Бесплатно
Статья научная
Potassium, the most abundant cation in plant cells, is responsible for numerous physiological functions. In saline environment, similarity of Na+ and K+ causes an unbalance in K+ uptake and disorder in the its functions. In the present research, changes of four biochemical parameters (proline, glycinebetaine, photosynthetic pigments and malondialdehyde) have been investigated in Aeluropus lagopoides seedling under salinity and potassium deficiency. Sterile seeds had been cultured on modified Murashige-Skoog containing 0, 1.75 or 100 mM potassium, with or without 600 mM NaCl for 30 days. The results showed that maximum proline content was observed in root and shoot by 600 mM NaCl + 1.75 mM K+ treatment. Also in this treatment, amount of carotenoids and chlorophyll a was more decreased. Potassium deficiency caused to reduced MDA and chlorophyll b content. The highest amount of glycinebetaine was measured in the presence of 600 mM NaCl in the company of 100 mM K+. It can conclude that chlorophyll oxidation was occurred in K+ deficiency because of increasing lipid peroxidation and disruption of protein-pigment complexes. The accumulation rates of two osmolite in different organ was shown that in A. lagopoides proline and glycinebetaine play more important role in osmotic adjustment of the shoot and root, respectively.
Бесплатно
Статья научная
The combined effect of cadmium stress (0,5 mM Cd(NO3)2) and salt stress (100 mM NaCl) on growth, lipid peroxidation and activities of some antioxidant enzymes were studied in soybean (Glycine max L. Merr.) leaves. Shoot lenghts were not changed under all groups. But fresh and dry weight were decreased under salt treatment alone and Cd treatment alone. The decrease was more in the group of combined of Cd and salt treatment. Although APX activity increased under salt treatment alone and combined of Cd and salt treatment, GR activity increased under Cd treatment alone and combined of Cd and salt treatment. Nevertheless DHAR activity increased only in combined of Cd and salt stress. MDA content increased under all groups but it was more in the combined of Cd and salt stress which indicates that salinity is more harmful with cadmium stress in soybean plants. Thus, it was concluded that some of antioxidant enzymes (APX, GR, DHAR) increased their activity under combined of Cd and salt treatment but they were not efficient to protect oxidative damage from soybean plants by alleviating the lipid peroxidation.
Бесплатно
Changes in serum enzymes levels associated with liver functions in stressed Marwari goat
Статья научная
Serum enzyme levels were determined in goats of Marwari breed belonging to farmers' stock of arid tract of Rajasthan state, India. The animals were grouped into healthy and stressed comprising of gastrointestinal parasiticised, pneumonia affected, and drought affected. The serum enzymes determined were sorbitol dehydrogenase, malate dehydrogenase, glucose-6-phosphate dehydrogenase, glutamate dehydrogenase, ornithine carbamoyl transferase, gamma-glutamayl transferase, 5'nucleotidase, glucose-6-phosphatase, arginase, and aldolase. In stressed group the mean values of all the enzymes increased significantly (p≤0.05) as compared to respective healthy mean value. All the enzymes showed highest values in the gastrointestinal parasiticised animals and least values in the animals having pneumonia. In gastrointestinal parasiticised animals maximum change was observed in G-6-Pase activity and minimum change was observed in malate dehydrogenase mean value. It was concluded that Increased activity of all the serum enzymes was due to modulation of liver functions directly or indirectly.
Бесплатно
Статья научная
In order to study the effect of re-drying temperatures on the enzymatic activation of hydro-primed Calendula officinalis (L.) seeds, a completely randomized design with three replications was conducted. Treatments were six different re-drying temperatures including control (without drying), 20, 30, 40, 50, and 60 ◦C applied after hydro-priming. Results showed that rapid drying at high temperatures (40-60 ◦C) caused a significant difference comparing control. The best re-drying temperature considering many studied traits was 20-30 ◦C. At 60 ◦C, activity of antioxidant enzymes such as superoxide dismutase, catalase and peroxidase decreased 65.5, 75, and 62%, respectively over control. Re-drying had not significant effect on the activity of malate synthase and isocitrate lyase in comparison with control and 20-30 ◦C that demonstrates no negative and reductive effect of re-drying with these temperatures on seed germination. It seems that seeds are more desiccation tolerant at these temperatures. In conclusion, we can dry hydro-primed seeds slowly at 20 and 30 ◦C and store them until utilization.
Бесплатно
Changes of body mass and thermogenesis in Apodemus chevrieri during cold exposure and rewarming
Статья научная
Environmental cues, such as temperature, play important roles in the regulation of physiology and behavior in small mammals. The aim of the present study was to test the hypothesis that ambient temperature was a cue to induce adjustments in body mass and thermogenic capacity in Apodemus chevrieri. It showed that A. chevrieri increased resting metabolic rate (RMR), nonshivering thermogenesis (NST) and energy intake and decreased body mass and body temperature when exposed to the cold while showed a significant increase in body mass and body temperature after rewarming. The decrease of body temperature can reduce the difference in temperature in environment, save energy consumption. The increase in body mass after rewarming was associated with the higher energy intake. Together, these data supported our hypothesis that ambient temperature was a cue to induce changes in body mass and metabolism in A. chevrieri.
Бесплатно
Статья научная
Process of cell death in suspension cultures of winter wheat and sugar cane under high (50 °С) and negative (-8 °С) temperature treatment has been studied. It has been shown, that programmed cell death (PCD) process caused by the negative temperature in the culture of winter wheat was noted for slow rate of realization and it was carried out for 10 days. It has been state that rate of cell respiration was significantly higher than in the control culture. At the same time PCD processes induced by the high temperature in the culture of sugar cane and winter wheat and by the negative temperature in the culture of sugar cane realized for 24-48 h and was accompanied by graduate decrease of respiration activities. We can conclude that the main reason of PCD processes realization differences was a different level of respiration metabolism resistance to high and negative temperatures action.
Бесплатно
Characterization of Medicago arborea L. response to water and salt stress
Статья научная
Medicago arborea L. is a Mediterranean leguminous fodder shrub, regarded as a promising species in arid and semi-arid lands where it can play an important role in the elaboration of a durable pastoral system. The aim of this paper is to investigate and characterize the response of M. arborea plants to water and salt stress at the early growth stage. Seedlings of the species derived from seeds collected in the Djelfa province, Algeria, were grown in pots under greenhouse conditions and separately submitted to water stress, restoring 20%,40%,60%,80% and 100% of substrate field capacity, and salt stress, supplying irrigation water with 0, 50, 100, 150 and 200 meql-1 of NaCl+CaCl2. Stress effects were determined on fresh and dry-matter biomass, relative water content, leaf pigment content (chlorophyll and carotenoids), proline and total soluble sugars amount. Results showed that both water and salt stress affected seedlings growth. In particular, the lowest water regime (20% of field capacity) significantly reduced fresh and dry-biomass and relative water content, whereas seedlings under salinity maintained a good water content (>70%).Chlorophyll a and b , and carotenoids content did not show significant differences among treatments, while proline and total soluble sugars amounts, major osmolytes involved in osmotic adjustment, significantly increased according to salt and water stress intensity...
Бесплатно
Characterization of induced mutants in short grain aromatic non-basmati rice Badshabhog
Статья научная
An experiment was conducted to evaluate the agronomic performance of advance generation 28 mutant families in the M4 generation. The mutant families were derived following pedigree method by treating aromatic short grain non-basmati land race Badshabhog with 200, 300, 400Gy doses of gamma rays. The mutant families retained the aroma of mother and were 10-15 days earlier. Agronomic performance reveals that height of many families was reduced which will render plants to be lodging resistant and responsive to higher doses of fertilizer. Some of the families have desirable mutant characters viz. flag leaf, panicle exsertion, panicle number and test weight. Majority of the families have reduction in panicle length, spikelet and grain number, spikelet fertility and grain yield which may be improved through gene cleaning. Segregation patterns of the mutant characters indicated that mutations are recessive and micromutations in nature. Appearance of multiple mutant characters in a plant may not be due to true mutations for all such characters, but may be due to pleiotropic effects of mutant genes. The mutants may be used directly and/or indirectly in recombination breeding programme for their beneficial mutant characters through Mutant × Parent and Mutant × Mutant crosses.
Бесплатно
Статья научная
Satureja spicigera (Lamiaceae) is an aromatic perennial plant and grows wildly in Black Sea Region of Turkey. This species traditionally has been used in traditional medicine, to treat various ailments and infectious diseases. The aim of this study was to compare the chemical composition the essential oil that was obtained from Satureja spicigera in different developmental stages in Central Black Sea Region, Turkey. Plant material was harvested at three different phenological stages (pre- flowering, full- flowering and post- flowering period) of the life span of the Satureja spicigera . The essential oil of S. spicigera was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. The hydrodistillation of aerial plant that were collected at pre- flowering, full- flowering and post- flowering period resulted in essential oil yields of 0.64%, 1.17% and 0.12%, respectively. Thirty-six compounds representing 90.83-97.81% of the oils were characterized. Throughout the growth stage of the plant, the main components were found to be carvacrol (32.77-49.11%), thymol (3.04 - 13.21%), γ-terpinene (3.11-13.33%) and ısothymol methyl ether (3.65-11.98%). While carvacrol content decreased during vegetation period, isothymol methyl ether level increased. Thymol and γ-terpinene reached that its highest values during full-flowering stage. The results obtained from this study showed differences compared to pre-flowering, full-flowering and post- flowering period on essential oil composition.
Бесплатно
Статья научная
Background: Invasive alien species (IAS) are broadly distributed all over the world by disturbing or reducing the growth and development of native vegetation. Lantana camara is basically a noxious weed and have a potential to invade a region's indigenous vegetation. Even after knowing all its harmful effects, there has been a little research on various mechanisms followed by this plant to harm the plant species. Results: Random sampling was performed to take plant samples without any bias, to study various mechanisms carried out in plant species. Chemical fingerprinting of samples were then performed in various abiotic stress conditions (cold and hot) to study changes in L. camara under these stressful conditions in order to find the reason behind the invasiveness of this plant species. Stress indicator like malondialdehyde (MDA)/lipid peroxidation was also performed and increased lipid peroxidation during both extremities showed that plant is experiencing oxidative stress...
Бесплатно
Chemical profiling of selected plants of Zingiberaceae used in Ethnomedicine of Koraput, India
Статья научная
The present study aims to quantify the phytochemicals present in ten selected species such as Zingiber roseum (Roxb.) Roscoe, Curcuma aromatica Salisb., Curcuma amada Roxb., Zingiber officinale Roscoe, Curcuma angustifolia Roxb, Alpinia calcarata (Haw.) Roscoe, Hedychium coronarium J.Koenig, Kaempferia galanga L., Curcuma longa L., Curcuma caesia Roxb of Zingiberaceae , which are commonly used in ethnomedicine by the tribal people of Koraput, India. The phenol, flavonoid, alkaloid, saponin, tannin, and ascorbic acid are major pharmaceutical parameters that are responsible for ethnomedicinal values were present in varying proportions. The plant species also contain significant amount of antioxidants that can be exploited as a potential source for herbal remedy for various diseases. Thus, public awareness and community-based programmes need to be encouraged at all levels for ex situ and in situ conservation of such species and will further use in exploration of new drugs for pharmaceutical industries.
Бесплатно
Статья научная
The present study was aimed to know the effect of aqueous extracts of the resurrection plant, Selaginella involvens(Sw.) Spring on spore germination in Pteris argyraeaT. Moore and also to know the ameliorating effect of the extracts on UV-Stress during spore germination of Pteris argyraeaT. Moore. Based on the present study it is concluded that the extract of Selaginella involvens, shows growth promoting effect by enhancing the spore germination in Pteris argyraea.Both UV and aqueous extracts of Selaginella involvensenhances germination, but the UV stress results in both physical and morphogenetic abnormalities. It is to be noted that in the extract treated spores, the physical abnormalities are in less frequency when compared to the extract- untreated spores.
Бесплатно
