Статьи журнала - Овощи России
Все статьи: 1472
Physalis peruviana L. production in conditions of the Crimean southern sea shore
Статья научная
Intensive climate changes entail the possibility of effective introduction of some tropical plants in the northern hemisphere. Introduction of Physalis peruviana, Indian selection, along the Crimean southern sea shore in 2022-2024 revealed the perennial growth character of plants, fully flowering since the first decade of June, starting fruiting in late June, full fruit ripening in the second decade of July, and the possibility of partial ovaries shedding during the period of high temperatures (2730°С) from mid-July to August. In the mentioned conditions, fruits, leaves and calyx showed high antioxidant status. The efficiency of different extraction methods in polyphenol determination generated high prospects of dry fruit, leaves and calyx extraction with 70% ethanol at 80 oC compared to the application of water, 50% methanol and 98% ethanol extraction at room temperature. The fruits demonstrated similar values of the ascorbic acid (48.5 mg/100 g f.w.), monoand di-saccharide (35 and 51% per d.w.) content, and higher levels of dry matter (20.2%), phenolics (21.2 mg GAE/g d.w.) and carotenoids (4.51 mg/100 g f.w.), compared to the Colombian fruit randomly sampled at the local supermarket, but had significantly lower values of fruit titratable acidity (49.0 compared to 86.2 mg-eq citric acid/g d.w.). Mineral composition of plants revealed typical Fe, Zn, Cu, Mn and Co distribution between fruit, leaves and calyx with calyx being the richest source of Fe (288.7 mg/kg d.w.). The results indicate high prospects of P. peruviana cultivation in Crimea.
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Plant viruses in the system of seed potato production
Статья научная
Solanum tuberosum L. is susceptible to 40 different virus species and 2 viroids. To prevent plant viruses from spreading in field conditions, it is necessary to have reliable data on the species composition of plant reservoirs of viral infection, the total activity of insect vectors, and possible ways of virus transmission in a particular territory of seed material production. Attention should be paid to the factors that facilitate and hinder the disease development in crops and to disease symptoms in different potato varieties. Manifestations of viral infections were monitored on every plant from the sample at the stages of initial growth, bud formation, and flowering and before the removal of potato haulms. Insects were collected using standard entomological method. The total RNA was isolated employing commercial kits for the extraction of nucleic acids from plant material “PhytoSorb” (Syntol Llc) and the benchtop automated extraction instrument KingFisher Flex (ThermoScientific) with magnetic particles. Plant viral infection was observed to accumulate if potato planting material was not renewed. The tested potato plants contained mixed viral infection, which consisted of viruses from mosaic group: PVY, PVX, PVM, PVS PVA, as well as PSTVd and PLRV. Without the renewal of seed potatoes, the concentration of plant viruses in an agroecosystem rises and causes secondary infections in potato plants. The research identified the main insect-vectors in the agroecosystem of potato fields: insects from genera Cicadella, Henosepilachna vigintioctomaculata, Dolycoris baccarum, Mythimna separata, Lygus pratensis, and Rhopalosiphum padi. Many wild weeds serve as fodder plants for insect vectors facilitating the accumulation of plant viruses in agroecosystems. It was established that perennial weeds were the main plant reservoirs of dangerous viral infections, e.g. Sonchus arvensis and Taraxacum officinale. We determined that Trifolium pratense typus L., Chenopodium album L., Plantago major L., Barbarea vulgaris W.T. Aiton, and Ambrosia artemisiifolia L. were the reservoirs of PVY. All these factors can lead to an epiphytotic situation.
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Potato viruses of 7 commercial cultivars grown in field Primorsky krai of Russia
Статья научная
Scientific relevance. Plant viruses cause a significant economic loss to potato production, especially if plants are infected at early growth stages and infections are mixed. Viral diseases reduce both yield and quality of harvested crops. Detection and identification of plant viruses are key important to prevent their spreading and to achieve potential yield predetermined by characteristics of varieties. Research methods. Seven potato varieties, bred in Russia and overseas, were used in the field experiment: Smak, Avgustin, Yantar, Laperla, Labella, Red Lady, Sante, Belmonda. Viral infection rate was measured by the percent of plants with symptoms to the total number of plants. In addition to infection frequency, a disease rate was described after visual estimation. Total RNA was isolated from the collected leaves according to Bekesiova I. et al. 1999 [13]. Qualitative and quantitative estimation of plant viruses in the samples were conducted by single-step real-time RT-PCR with fluorescent detection with the Applied Biosystems QuantStudio 5 and commercial kits “Potato Virus X, Y, M, L, S, A, PSTVd-RT” (Syntol Company) according to the official protocol of the kits. Results. As a result of our research, symptoms of mixed viral infection were described for potato varieties depending on concentrations and proportions of these viruses in a plant. Mixed viral infection in the potato field in Primorsky Krai comprised PVY, PVX, PVA, PVS, PVM, also PLRV and PSTVd.
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Potential medicinal plants used in the treatment of COVID-19: A review
Статья научная
Novel coronavirus COVID-19 (SARS-CoV-2), the unexpected pandemic that been caused severe fright worldwide. It has presented the world with one of the most difficult global public health crises and the arrival of COVID-19 has kept the whole world on their toes. The spread of COVID19 has become a health emergency and attention has been raised worldwide to design prevention and management strategy. Although several clinical trials are ongoing, no approved medications from Food and Drug Administration are available at a time, after while some preventative vaccines have been developed, manufactured and deployed depending on variant of COVID-19. As situation warrants for the exploration of a successful antiviral, there should be a search for the remedies in nature medicine. Medicinal plants and their metabolites have long been used as a treatment option for various life-threatening diseases with minimal side effects. Thus this review aims to summarize previous outcomes concerning the role of medicinal plants in treating several life-threatening diseases for the potential medicinal plants used in the case of COVID-19 treatment. Some of these includes Turmeric (Curcuma longa Linn.), Black Cumin (Nigella sativa L.), Garlic (Allium sativum L.), and Ginger (Zingiber officinale Rosc.). These are important traditional herbal medicines to cure many complicated health ailments. However, further extensive researches and trials are suggested to discover the role of medicinal plants for management of the pandemic. Moreover, the use of potential medicinal plants for specific variant of COVID-19 and others life-threatening diseases has to be investigated.
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Primary metabolites and betanin: their interplay in the roots of table beet (Beta vulgaris L.)
Статья научная
Relevance. The main source of the natural pigment betanin is table beet, known for its medicinal and antioxidant properties, earliness and long shelf life, rich in bioactive compounds, minerals and vitamins. This research was induced by the lack of information required by breeders to increase betanin content in beet. Metabolite profiling is an effective way to assess the interplay between individual metabolites and betanin content in table beet. Materials and methods. The material was selected from the the N.I. Vavilov Institute of plant industry (VIR) collection. Biochemical analysis was based on VIR’s guidelines, and metabolite profiling on gas chromatography, coupled with mass spectrometry. Results. 17 free amino acids were found in the beet root extract. The greatest number of positive correlations with other amino acids (r>0.72) was found in tyrosine, alanine and phenylalanine. A significant (r = -0.66) negative correlation was observed between betanin and succinic acid, credibly associated with betalamic acid. Sucrose predominated among carbohydrates (95%). Sucrose and maltose showed a weak positive correlation with betanin. Unsaturated oleic and saturated palmitic acids dominated among fatty acids (52% and 20% of total fatty acids, respectively). Earlier-ripening and cold-resistant table beet accessions showed a predominance of unsaturated fatty acids and lower betanin content. The disclosed interactions are important for betanin-rich red beet breeding
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Production and marketing challenges of tomato (Solanum lycopersicum L.) in Ethiopia. Review
Статья обзорная
Tomato is one of the most important and widely grown vegetables in the world. The aim is to review the key challenges on tomato production and marketing in Ethiopia. In Ethiopia, tomato production is concentrated in river, valleys and lakes, especially in Awash Valley and around Lake Ziway for favorable growing conditions, good access to market outlets and better infrastructure. Tomato production is commercially important for fresh fruit market and processing. Some of tomato varieties that have been in use by farmers are used for processing and for fresh marketing. However, several constraints caused inconsistent of tomato production and low yielding’s are; the shortages of improved varieties, inadequate transport, poor marketing system, poor cultural practices, unreliable rainfall, price fluctuation, product nature (perishability), post-harvest losses, pest and diseases. Lack of market linkages, post-harvest losses, low institutional support, lack inputs and transportation are the key challenges. Small-scale producers are struggling to gain market access, but due to listed challenges the farmers are not selling their produce in an organized system and not getting the right shares. Therefore, critical attentions in harvesting and postharvest operations are very important to reduce losses, to keep quality and market standards. Moreover, addressing both production and market-related challenges are essential to minimize the losses, to access quality goods and to ensure the right shares for producers, distributors, processors and traders.
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QTL анализ биохимических признаков качества у brassica rapa l
Статья научная
С использованием двух картирующих популяций линий двойных гаплоидов Brassica rapa (DH30, получена скрещиванием корнеплодной репы и масличного желтого сарсона и DH38, получена скрещиванием листовой/черешковой капусты китайской и желтого сарсона) был проведен QTL (quantitative trait loci) анализ пяти биохимических признаков качества. Для каждого изученного признака впервые в России установлены QTL, эффекты действия выявленных QTL, доли фенотипической изменчивости, определяемой каждым QTL, и молекулярные маркеры, генетически сцепленные с идентифицированными QTL. Найденные молекулярные маркеры могут служить эффективным инструментом при массовом скрининге образцов коллекции и селекционного материала по биохимическим признакам качества.
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QTL анализ и управление продуктивностью растений в системе точного земледелия
Статья научная
Современные технологии возделывания сельскохозяйственных культур достигли пределов «насыщения» как в экологическом (загрязнение природной среды, подавление механизмов ее саморегулирования), энергетическом (экспоненциальный рост затрат невосполнимой энергии на каждую дополнительную единицу продукции), так и в продукционном. В этой связи все большее значение в обеспечении устойчивого роста урожайности возделываемых форм растений приобретают факторы внешней среды (воздушная засуха, морозы, активные температуры и т.д.), оптимизировать которые невозможно. В последние десятилетия все больше внимания уделяется техногенным и биологическим системам земледелия, основанным экологизации и биологизации интенси-фикационных процессов адаптивного растениеводства. Такими подходами является система точного земледелия (ТЗ) и QTL анализ. Использование этих подходов позволяет не только обеспечить устойчивый рост урожайности за счет совокупного использования преимуществ точного земледелия и молекулярно-генетической оценки, включая создание новых форм и сортов отзывчивых на агроприемы ТЗ, но и нивелировать отрицательное влияние абиотических и биотических факторов среды, лимитирующих величину и качество урожая, а также продуктивность растений. Показано, что стратегия адаптивной интенсификации растениеводства за счет использования системы ТЗ и подходов QTL анализа не является альтернативной по отношению к существующим системам земледелия, однако она ориентирует современное сельское хозяйство на рост наукоемкости сельскохозяйственного производства в целом. Анализ рассматриваемых причин существующих на сегодня неблагоприятных тенденций в современном растениеводстве и земледелии, со всей очевидностью показывает их масштабность и долговременный характер, а следовательно, и неизбежность поиска новых приоритетов интенсификации растениеводства и земледелия, обеспечивающих качественно новый этап их развития в интересах человека.
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QTL анализ морфологических признаков качества у brassica rapa l
Статья научная
С использованием линий двойных гаплоидов двух картирующих популяций Brassica rapa L. проведено морфо-биологическое изучение и картирование QTL (quantitative trait loci), определяющих проявление ряда морфологических признаков качества в условиях теплицы. Идентифицированы и локализованы на группах сцепления QTL таких признаков качества, как длина, ширина, окраска, опушенность, характер поверхности листовой пластинки, а также длина и ширина черешка, обуславливающих питательную ценность данного вида растений. Выявлены молекулярные маркеры, генетически сцепленные с картированными QTL, и установлен процент фенотипической изменчивости, определяемый идентифицированными локусами хромосом.
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Regenerative ability and micropropagation of Petunia hybrida in vitro
Статья научная
Scientific relevance. The garden petunia, Petunia hybrida, is a popular and wide spread ornamental crop from the family Solanaceae. It is a promising model plant for molecular and genetic research. In vitro micropropagation plays an important role in the distribution of the garden petunia because the survivability and quality of seed material decreases significantly in every subsequent generation. Besides, micropropagation reduces the cost of production substantially. Considering that very few researchers addressed this question in the Russian Federation, this direction of research is still worthy of attention. Materials and methods. The experiments were conducted by the Laboratory of Breeding and Genetic Research on Field Crops at FSBSI “Federal Scientific Center of Agricultural Biotechnology of the Far East named after A.K. Chaiki”. Seeds of Petunia hybrida (double-flowered) were used as primary explants. Liquid bleacher ACE diluted with distilled water in the proportion 1:9 was used as a sterilizing agent (the working solution contained 0.50% NaOCl). The total time of exposure was 15 minutes. The primary explants were subcultured onto a hormone-free Murashige and Skoog basal medium containing 20 g/L sucrose and 6 g/L agar. Isolated in vitro objects were cultured in test tubes with cotton-gauze plugs at an illuminance of 4000 lx, a temperature of 22-25 °C, and a 16h photoperiod in a culture room. The duration of one passage was 60 days. Micropropagation was carried out using 710 mm cuttings with one or two nodes. The pot culture of the regenerants was established under controlled conditions in a light room (photoperiod was 16 hours, temperature was 23°С). Results. The optimal method for introducing Petunia hybrida into cell culture is the use of seeds treated with the solution of bleacher ACE that was diluted with distilled water in the proportion 1:9. The optimal time of exposure is 15 minutes. Petunia hybrida demonstrated a high regeneration rate on the hormone-free MS medium - it had a fast growth and development rate, and good rhizogenesis; the reproductive rate was 8.77. For the micropropagation of the garden petunia, it is advisable to use cuttings of test tube plants, which should be placed onto a hormone-free MS medium. The test tube plants of Petunia hybrida acclimatized successfully on a soil substrate. This shows the high plasticity of the culture.
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Review on drivers of deforestation and associated socio-economic and ecological impacts
Статья научная
Relevance. Forests play a vital role in maintaining ecological balance and providing habitats, oxygen, and carbon sinks. However, deforestation resulting from mining, logging, infrastructure development, climate change, and agriculture is leading to their depletion. This paper aims to investigate the underlying factors driving deforestation and their socioeconomic and ecological consequences. Results and Discussion. Deforestation has resulted in the loss of 10 million hectares of global forest cover annually, with direct drivers including fires, logging, and converting forests to agricultural land. Indirect drivers encompass political elections, military conflicts, population density, and corruption. Economic growth, road expansion, and politics have exacerbated the pressure on forests, resulting in biodiversity loss. Deforestation is predicted to continue at a rate of 10 million hectares annually until 2025. To mitigate this issue, measures such as reducing emissions from deforestation, promoting sustainable forest management, and enhancing protected area management can be implemented. Furthermore, legal action should be pursued to address the issue of deforestation.
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Статья научная
Relevance. There are very few studies on the role of plant viral infection in inhibiting the immune response of potato plants to the damage caused by leaf-eating pests; the available data has a fragmental character. Materials and Methods. The research was carried out in an experimental field of FSBSI “FSC of Agricultural Biotechnology of the Far East named after A.K. Chaiki” in 2020-2023. The following potato varieties of Russian and foreign breeding origin were used in the experiment: Belmonda, Sante, Dachnyi, Yantar', Avgustin, Yubilyar, Kazachok, Red Lady, Labella, Queen Anne, Laperla, Smak, Lilly, Arktika, Svitanok Kievskii, Nakra, Dal'nevostochnyi, and Severnyi. The progression of plant viral infection and the degree of the damage caused by Henosepilachna vigintioctomaculata were assessed on a point scale. Results. The research established a direct correlation between a decrease in the immune response of potato plants to leaf-eating insects and the accumulation of viral infection without the renewal of planting material. Potato varieties Svitanok Kievskii, Nakra, Dal'nevostochnyi, and Severnyi were observed to have latent viral infection (from 0 to 0.5 points) in the first year of the experiment (2022) and were not susceptible to the potato ladybird beetle. Mixed plant viral infection manifested itself on potato plants in 2023 decreasing the immunity of the plants to phytophagous insects, particularly to the potato ladybird beetle. The progression of plant viral infection on variety Svitanok Kievskii reached four points while the degree of the damage caused by the potato ladybird beetle to potato plants was 1.2 points. The progression of plant viral infection scored about two points on varieties Nakra and Severnyi with a damage degree of one point. Variety Dal'nevostochnyi was the least susceptible to plant viral infection and the degree of damage was minimum as well.
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Статья научная
Relevance. Applying exogenous auxins to the cuttings of grapevines is a common practice in viticulture to improve the rooting process. The potassium salt formulations of auxins have been documented to be more, less, or equally efficient as acid formulations in rooting stimulation of cuttings depending on the genetic features of the plant, type and concentration of auxin, type of cuttings, and many other factors. Methods. The present study aimed at evaluating the effect of potassium salt of indolyl-3-acetic acid (KIAA) on rooting of the hardwood cuttings of two grape cultivars namely, Korinka russkaya and Victoria, which are commonly planted by grape growers of the Tatarstan Republic. The study was conducted in 2018 and 2019 under hydroponic conditions in a controlled-environment growth chamber in Kazan State Agrarian University. The cuttings were treated with 1) KIAA at two concentrations (5,000 and 10,000) ppm; 2) β-indolyl-butyric acid (IBA) 3,000 ppm (as a check treatment) and 3) distilled water as a control...
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Rooting technique of double haploids obtained in culture of microspore in vitro for European radish
Статья научная
Relevance. Doubled haploids (DH-plants) are excellent material for genetic research and breeding due to their complete homozygosity. The genus Raphanus from the Brassicaceae family is the toughest to produce doubled haploid plants through isolated microspore culture in vitro (IMC). The study of the causes of disturbed root formation and the development of elements of this stage of technology will significantly increase the effectiveness of the IMC technology for European radish. Methods. The study included three varieties from the collection of the Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (FSBSI FSVC): Teplichny Gribovsky, Rozovo-krasniy s belim konchikom and Rhodes. The experiments used a standard protocol for obtaining DH plants using IMC technology in a standard form and with a modification of the rooting stage. The solid MS medium (with agar 7g/L): MS without hormones, MS medium supplemented with IAA at concentrations of 0.5; 1 and 2 mg / L and liquid MSm medium supplemented with 0.1 mg / L kinetin were used for rooting of regenerated plants. All media were supplemented with 20 g/L sucrose. We used three types of techniques for transplanting plant explants onto a solid hormone-free MS medium: planting micro-shoots with their basal part immersed by 2-3 mm into the medium; planting in a well made in a nutrient medium using tweezers under sterile conditions; and landing on the surface of the medium without embedment. Results. In this work, we studied the features of the stage of rooting of regenerated European radish plants in vitro conditions. The transplant technique has been proven to be important for the successful establishment of radish micro-shoots. Plant explants must be planted strictly on the surface of a solid hormone-free nutrient medium MS, without embedment. The use of tubes with bridges made of filter paper and MSm liquid medium with the addition of 0.1 mg/L kinetin for the induction of root formation also showed high efficiency. For plants prone to the formation of root-like structures (RLS) with secondary tumors (ST), multiple dissection of abnormal formations with successive transplants s necessary. Modification at the rooting stage of micro-shoots growing has increased the percentage of successfully adapted DH plants in vivo conditions from 0-14% to 95-98%.
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Статья научная
Relevance. Scutellaria baicalensis Georgi is a valuable medicinal plant, its raw material is roots. It is a protected species of the East Asian flora of the Russian Federation. The plant is included in 6 Red Data Books of the Far East and Eastern Siberia. Works on introduction of Scutellaria baicalensis carried out in many regions of the Russian Federation. At present, multifaceted biochemical studies of the flowering shoot mass of Baikal skullcap are being carried out everywhere. The aim of the investigation is study of some biochemical parameters of flowering shoot mass of Baikal skullcap by structure in different weather conditions in different years. Materials and methods. The object of the study was the population of Baikal skullcap from the biocollections of All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (Central Region of the Non-Chernozem belt). Studies were conducted in 2018 (optimal weather conditions) and 2020 (stresses weather conditions). The biochemical composition of the shoot of Scutellaria baicalensis studied in the Laboratory and Analytical Department of the FSBSI “Federal Scientific Vegetable Center” according to the following parameters: dry matter, ascorbic acid, total content of water-soluble antioxidants and total antioxidants in the alcoholic extract. The accumulation of these substances done in leaves located on the upper, middle and lower levels of leaves, inflorescences and buds, stems. Results. Dry matter content of Scutellaria baicalensis herb components did not differ significantly by years. The maximum meaning of this indicator was in stems – 38.89-39.51 %, and the minimum – in inflorescences – 21.07 %, regardless of the level of location on the shoot. The total content of water-soluble antioxidants in the shoot mass under optimal precipitation was statistically significantly higher than this factor under dry weather conditions by 1.5-2.7 times. In inflorescences and buds the content of water-soluble antioxidants was lower than on average in leaves by 2.6-3.6 times. Ascorbic acid accumulation in leaves of S. baicalensis in a weather-optimal year (2018) exceeded its content in a year (2020) with numerous extremely changes in precipitation and air temperature during the growing season by 1.5 times. The total antioxidant content in the alcoholic extract in the shoot mass in the year with significant fluctuations in weather conditions (2020) was significantly higher than that in the year with stable weather conditions (2018). In both years of the study, the maximum value the total content of antioxidants observed in the leaves. In a weather-optimal year, these were the leaves of the lower level (77.52 mg-eq GA/g d.w.), and in a year with extremely fluctuations in weather parameters (2020), these were upper level (83.05 mg-eq GA/g d.w.).
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Study of saving and investment pattern on sample farms households
Статья научная
The saving and investment pattern of different forms sample group was studied during 2014-16 and it was observed that large farm holders were able to save higher income than small farmers while lowest income group had negative savings. In respect of investment on different fixed assets, irrigation was on first priority, followed by purchase of milch animals, farms buildings and investment in land and its improvement. Investment on working capital amongst different cash inputs, hired human labourer accounted highest share (29.44 per cent), followed by manure & fertilizers (22.33 per cent), hired power tractor (16.96 per cent), irrigation (13.61 per cent) and seeds (13.50 per cent) to total cash inputs. Marginal farmers could not invest for nonfarm physical capital because of no savings with them. Small and large farmers groups invested in all the items in which it was highest in working capital (61.28 to 61.84 per cent), followed by investment in fixed capital (14.41 to 16.84 per cent), financial capital (12-14 per cent) and non-farm capital (7-12 per cent). The highest investment was made on working capital (69.02 per cent) by sample farmers. Current income was found to be the main source of finance in all income groups which accounted for 49.70 to 94.79 per cent share of the total investment followed by savings which shared for 40.10 to 49.12 per cent in total investment.
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Study on quantitative characters of organic seeds in garden pea
Статья
The experiment was conducted in the Maritsa Vegetable Crops Research Institute, Plovdiv during the period 2008-2009. Four garden pea varieties were grown in the conditions of organic and conventional production. The purpose of the experiment was to study the quantitative characters of seeds obtained in organic production. It was established that the organic garden pea seeds differ with lower absolute weight compared to the seeds obtained in conventional system but they kept high germination (89,5-96,5) close to the control variant. The late variety Vyatovo grown with organic fertilizers and biopesticides are with the lowest percentage of injuries by Bruchus pisi l.
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Статья научная
Relevance. Tomato pink-fruit cultivars and hybrids are of interest for fresh consumption. The biochemical parameters are influenced by the varietal factor and by the growing conditions. It is known that mature tomatoes record low storability. The softening of the fruit is caused by ethylene produced in the ripening fruit. Then pectinase enzymes are synthesized in the fruit tissues, under the action of which the cell walls of the fruit soften. Breeders are creating tomato cultivars whose fruits could be stored for a long time without losing their tasty. The aim of the investigation was to study the biochemical parameters of pink-colored tomato fruits under different ripening conditions.
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The effect of grafting on vegetative and reproductive traits of tomato
Статья научная
Relevance. Tomato (Solanum lycopersicum L.) is one of the most important vegetables in the world. Every year the number of identified viruses and diseases increases, infection with which causes significant crop losses and significantly worsens the quality of agricultural products, especially in tomatoes. Material and methods. In the present study we compared the growth of the vegetative and reproductive traits of tomato plants ‘Dokia’, ‘TY Red 250’ and ‘Pilabi’ grated onto bacterial wilt (caused by Ralstonia solanacearum) and Fusarium wilt (Fusarium oxysporum) diseases tolerant rootstock “Spider” (Takii seed, Japan). The non-grafted (control - CT) and grafted tomato plants (GR) were transplanted on 4 March of 2021 to the four season (vinyl) greenhouse on substrate perlite (mixture of perlite no.1 and no.3) and cultivated until September 20.
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