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Статья научная
Corypha umbraculifera L. is a monocarpic palm, which usually flowers after 30-60 years of growth. In C. umbraculifera seeds are the only propagating unit, but the germination rate is very low and seeds are highly recalcitrant. In this context, it was imperative to investigate the desiccation sensitivity, physiological and biochemical changes accompanying the desiccation and germination in C. umbraculifera seeds. Therefore, to make a detailed study, freshly collected C. umbraculifera seeds were desiccated at room temperature for a period of 35 d and physiological and biochemical changes during desiccation and germination were monitored at an interval of 7 d. It was observed that there was a sharp decline in the moisture content of the seed as desiccation proceeded. As the desiccation period progressed, the germination percentage decreased which was below 50% after 35 d. The dry weight percentage of the embryo and endosperm increased with the desiccation period and the increase in dry weight of embryo was significant in comparison with the endosperm. Total protein content of embryo was more compared to that of the endosperm. Peroxidase activity in the embryo was increased up to 28 d of desiccation and decreased further. The endosperm registered a gradual reduction of peroxidase activity during desiccation. In contrast, SOD activity in the embryo was comparatively higher in the fresh seeds and further declined during desiccation, while that of the endosperm remained almost unaltered. The results give a strong indication that desiccation in C. umbraculifera is accompanied by abundant activity of peroxidase in embryo, thereby viability is retained up to 35 days. Whereas, feeble activity of SOD is not seen to be linked with seed viability of C. umbraculifera.
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Статья научная
One year old seedlings of Simarouba glauca were subjected to water stress for 4, 8, 12 and 16 days. The foliar sprays of 50 ppm salicylic acid (SA) and 10 ppm Putriscine, Gamma amino butyric acid (GABA) and Abscisic acid (ABA) were applied before and after water stress. It was observed that polyphenols, tannins, alkaloid and flavonoid contents were increased with increasing water stress treatments. Foliar applications of growth regulators ameliorate water stress and exhibits induction of secondary metabolites like coumarins, sterols, xanthoproteins, cardiac glycosides and saponins. It was also noticed that foliar application of SA, GABA, ABA considerably increases all these secondary metabolites which will help to improve the medicinal potential of Simarouba glauca under water stressed condition.
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Статья научная
The data on the influence of different concentration of sodium nitroprusside (SNP), potassium nitrate (KNO3), sodium nitrite (NaNO2) and L-arginine on 3 days-old etiolated pea seedlings growth and nitric oxide (NO) content in roots of these seedlings are obtained. It is found that 24 h exposition of seedlings to SNP showed negative influence on growth at a 4 mM dose; SNP at 0.05 mM doze stimulates their growth and 0.1 mM doze did not influence on seedlings growth. Using KNO3 negative influence on growth at the same exposition was showed only at superhigh concentration - 20 mM. Using NaNO2 negative influence on growth was shown at a doze of 2 mM but concentration of 0.1 and 0.5 mM did not influence on it. The exposition of seedlings to L-arginine caused growth inhibition already at concentration of 0.5 mM and reached maximum at 4 mM doze. Determination of NO level in roots using fluorescent probe DAF-2DA in variants with the greatest growth inhibition has showed, that the maximal inhibition of growth in roots and highest level of NO in roots in variants with SNP (4 mM) and NaNO2 (2 mM) was observed. In variants with KNO3 (20 mM) and L-arginine (4 mM) maximal growth inhibition did not coincide with NO accumulation and was observed after 30 min after the beginning of plants exposition and further it was reduced in 24 h. Results are discussed in connection with possible influence NO and participation of the investigated connections in generation nitric oxide in roots of pea seedlings.
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Статья научная
The influence of treatment of millet (Panicum miliaceum L.) seeds with the solutions of salicylic and succinic acids on the heat resistance of plantlets and activity of antioxidant enzymes - superoxide dismutase (SOD), catalase and peroxidase - in them have been investigated. In the micro-field experiment the influence of these acids on the millet yield was estimated. The action of salicylic (10 μM) and succinic (1 mM) acids caused the increase of plantlets resistance to the damaging heating that expressed in the rise of relative quantity of survived plantlets in 5 days after heating at the temperature of 47°С and in the reduced content of lipid peroxidation product malonic dialdehyde during the poststress period. The increase of activity of SOD, catalase and peroxidase took place in millet plantlets under the influence of salicylic and succinic acids. The increase of productivity of millet grain under the action of salicylic and succinic acids on 13,3-52,0 and 6,4-38,8% respectively depending on weather conditions in the field experiments was noted.
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Influence of salinity stress on proteomic profiles of Cicer arietinum L
Статья научная
The present study was aimed to study the influence of salt stress on the proteomic profiles of Cicer arietinum L. by using SDS-PAGE. Seedlings of Cicer arietinum were exposed to different salt concentrations (4%, 6%, 8% and 10%) of NaCl. Multiple regions of actively stained system were obtained for SDS-PAGE. On 15th day, a total of 79 bands were observed and their RM values ranged from 0.011 to 0.988. Based on the occurrence and non-occurrence of the proteins in the gel system, the protein profiles were classified in to three categories viz., salt tolerant proteins, salt inducible proteins and salt sensitive proteins.
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Influence of slavinorm on resuscitation of rats cardiorespiratory functions under deep hypothermia
Статья научная
The problem of resuscitating a man whose respiration and heart were arrested under strong cooling is urgent for the reanimation practice. The aim of this work was to study the influence of Slavinorm (a polypeptide complex of the vessels) on the heart work activation and the regeneration of their inherent respiration in the Wistar rats with the help of artificial ventilation of lungs (ALV) and warming the sternum region under deep hypothermia. The animals narcotized with urethan were cooled in water (9,2±0,5ºС) up to the arrest of respiration. The experimental group of rats 1 h before cooling were injected intraperitoneally with Slavinorm (15,6 mg/kg of weight) and the control rats were injected with physiological solution. In control rats the arrest of respiration was observed at the rectal temperature (Тr) 16,0±0,3ºС, in the esophagus (Te) 19,1±0,3ºС. The artificial lung ventilation (ALV) and heating the breast region resulted in the initiation of natural respiration in 29,2±0,4 min from the beginning of ALV at Tr 13,8±0,2ºС, Тe 16,1±0,2ºС. The conclusion: Slavinorm increases the resistance of an organism to cold, assists the prolongation of the process of cooling up to the arrest of respiration, decreases the temperature thresholds of the heart activation and resuscitation of natural respiration.
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Статья научная
This study involves the evaluation of in vitro shoot regeneration during cotyledon culture in black sesame ( Sesamum indicum L. cv. TMV3) under various concentrations of sucrose and cytokinin (BAP) in Murashige and Skoog (MS) nutrient medium. Cotyledon explants were cultured on MS basal medium supplemented with various concentrations of sucrose (2%, 4%, 6%, and 8%) and combinations of BAP (1.0mg/L, 2.5mg/L, 3.5mg/L, 4.5mg/L, 5.5mg/L, 6.5mg/L, and 7.5mg/L) and IAA (1.0mg/L). The multiple-shoot regeneration was achieved after 5-weeks of culture initiation. Significantly, the maximum mean percentage of shoot regeneration (75±4.3%) and number of shoots/explants (1.3±0.8) was recorded with explants that were treated with MS medium supplemented with sucrose (4%) including BAP (3.5mg/L) and IAA (1.0mg/L) while the high concentration (8%) of sucrose was proved to be completely ineffective to induce shoot regeneration on same nutrient medium (BAP+IAA). Furthermore, results indicate that sucrose (8%) could be observed slightly effective for shoot regeneration with higher concentrations of BAP (6.5mg/L and 7.5mg/L) in presence of IAA (1.0mg/L). Significantly, cotyledon explants that were growing with low concentration of sucrose (2%) were failed to show regeneration if BAP concentration exceeds to (5.5mg/L). Moreover, optimal frequency of shoot regeneration (64.4±1.4%) could be recorded with 2% of sucrose in presence of lower concentration of BAP (2.5mg/L) and IAA (1.0mg/L). The regenerated shoots were further transferred to half strength of MS medium supplemented with various concentrations of IBA (0.5, 1.0, and 1.5mg/L) for induction of roots and complete plantlets formation. Significantly, optimal mean percentage of shoots showing root formation (90±2.8%) was obtained in shoots regenerated with 4% of sucrose in presence of IBA (1.0mg/L) and shoot-root length ratio was recorded to be the maximum (4.2±0.11cm/2.81±0.12cm). However, in contrast, shoots that were regenerated on medium containing high sucrose (8%) with high BAP (7.5mg/L) + IAA (1.0mg/L) were found to exhibit lack of root formation irrespective of IBA concentrations. Present study reports that sucrose and BAP contents of the medium influences the sesame cotyledon explants regeneration. In comparison to control (2%) of sucrose, increase in sucrose concentration (4%) with BAP (3.5mg/L) and IAA (1.0mg/L) proved to be the optimal combination for shoot regeneration and moreover, regenerated shoots could induce the maximum root length in MS1/2 medium supplemented with IBA (1.0mg/L). In contrast, the IBA medium containing very high concentration of sucrose (8%) was turned out to be completely inhibitory for root regeneration. Study indicates that concentration of sucrose and BAP cytokinin in nutrient medium influences considerably the regeneration potential of explants tissues in black sesame oil crop. Moreover, the regenerated plantlets were further gradually acclimatized and transferred to plastic cup soil under the greenhouse conditions.
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Статья обзорная
Influence of expressed leghemoglobin on stability of Escherichia coli cells to reactive oxygen and nitrogen species - fert-butyl hydroperoxide and S-nitrosoglutathione was studied. We determined both action of these compounds on cells with different level of leghemoglobin expression and peroxidase activity of the cells. It was shown that priority of pro- or antioxidant properties of leghemoglobin is dependent on its concentration in the cells. In cells with reduced synthesis leghemoglobin mostly functions as prooxidant, in cells with intensive one - as antioxidant
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Influence of waterlogging on carbohydrate metabolism in ragi and rice roots
Статья научная
Effect of different durations of waterlogging (4, 8 and12 days) stress on carbohydrate status and activities of some related enzymes in ragi and rice roots was studied. In both ragi and rice roots there was decrease in starch and total sugar content in response to waterlogging conditions. Activity of α amylase was decrease in ragi roots while opposite trend was noticed in case of rice roots. The activity of pyruvate kinase was markedly increased due to 4, 8 and 12 days waterlogging in ragi roots while such increase was noticed in rice roots due to 12 days stress. Treatment of waterlogging caused enhancement in the activity of alkaline inorganic pyrophosphatase in the roots of both ragi and rice.
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Integrative computer analysis of antisense transcripts and miRNA targets in plant genomes
Статья
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Статья научная
This research was carried out to study the effect of Tio2 and nano Tio2 on some chemical compounds in a medicinal plant Lallemantia iberica under water deficit conditions. The experiment was conducted as a factorial arrangement in randomized complete block design with three replications. The first factor was the application of external materials as spraying with 3 levels of control, titanium dioxide and nano titanium dioxide, and the second factor was 100% (non stress), 75% (moderate stress) and 35% (severe stress) irrigation content. Stress was applied sixty days after planting to harvesting stage. The catalase and superoxide dismutase activities, total phenol, antioxidant activity with DPPH, total flavonoid and HPLC of phenolic compounds were evaluated in the samples. The results showed a moderate stress and the use of nTiO2 caused a significant increase in phenolic content, total flavonoid and antioxidant activity; severe drought stress significantly reducing these compounds...
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Interactive effects of rice residue and water stress on growth and metabolism of wheat seedlings
Статья научная
In the present study effects of rice residue with and without water stress were studied on Triticum aestivum L. cv. Shatabadi. The mixture of residue and garden soil in 1:1 ratio was considered as 50% (R 1) and only decomposed residue as 100% (R 2). Garden soil was taken as control. Twenty five seeds were sown in each experimental trays filled with soil mixture according to the treatments. Trays were arranged in two groups. After 15 days one set was subjected to water stress (WS) by withholding water supply for 3 days. Morphological and biochemical parameters of 18 days old seedlings were recorded. Seedling height decreased in all treatments. A gradual decrease in relative water content, pigment and protein contents of wheat seedlings were observed. Sugar and proline contents increased in treatments. An increase in malondialdehyde (MDA) content and antioxidative enzyme activities was recorded. Elevation in catalase activity was observed in all treatments except in plants with water deficit. Ascorbate peroxidase (APX) and guaiacol peroxidase (GPX) activities increased when residue mixed with soil but decreased in seedlings under the combined influence of the residue and water stress. Higher amount of MDA and lower activities of APX and GPX reflected the oxidative damage in seedlings under combined treatments. Rice residue inhibited growth of wheat seedlings. Water stress intensified the effects of residue.
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Статья научная
Houttuynia cordata Thunb. is a plant enrichment in potassium in plant was reported. Salinity and low potassium availability are important environmental factors restricting plant growth and productivity throughout the world. The interactive effects of salinity and potassium on growth, water content, chlorophyll content, lipid peroxidation content, ion accumulations and K+/Na+ ratio, and organic accumulations as well as oxidative enzymes were investigated in Houttuynia cordata Thunb.. Plants of three-leaf-stage were selected for uniformity, then treated with four levels of Na+ (50, 100, 200 mmol/L) and K+ (0, 0.6, 1.2, 2.4 mmol/L) for 20 days. Plant biomass production, ratio of root and shoot, root numbers, water content and MDA content significantly declined in the combined effect of salinity and K+ deprivation, and increased with salinity. However, salinity in conjunction with K+ deprivation led to an increase on leaf chlorophyll content, which even increased with increasing salinity levels. As expected, K+ content in plant was positive correlated with supplementary K+ concentrations, while Na+ was well correlated with salinity, especially enhanced by the interactive effects of salinity and K+ deprivation. Soluble sugar and proline contents remarkable increased by the highest salinity. SOD activity also substantial increased by the highest salinity, and increased with supplementary K+ concentrations. However, elevated CAT and POD activities were not accompanied with an increase in SOD activity.
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Статья научная
Hydrolytic enzymes of Bacillus attracting a great attention due to their various applications in industrial bioprocesses. In this work, out of 525 Bacillus isolates, 40 were screened for their potential capacity to produce hydrolytic enzymes including xylanase, lipase, amylase, pectinase, protease and carboxymethyl cellulose (CMC) using agar plate assay. A pure culture of each isolate was streaked on the surface of agar media containing suitable substrate specific for every enzyme activity, and the diameter of hydrolytic zones were measured. Data showed clear zones around the colonies which were interpreted as evidence of the enzymes activities. The isolates were able to generate at least one of these enzymes among which CMC and protease were the most common enzymes detected in 40 isolates; amylase in 39, xylanase in 36, lipase in 32 and pectinase in 26. Based on 16S rRNA gene sequencing the isolates were classified as B. atrophaeus , B. amyloliquefaciens , B. subtilis , Paenibacillus polymyxa , B. simplex and B. tequilensis . The native B. tequilensis isolates formed the largest zone clearance and had high abilities to produce five hydrolases. The isolates which had the largest enzymatic activity zones and the largest diameter of the clear hydrolysis zones on agar plates were submitted for further research work and enzyme-based industrials.
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Investigation of brain creatine levels under the mental stress conditions
Статья научная
Alterations in brain creatine levels are considered to be associated with various pathological conditions. However, there is still no exact evidence on character of this changes and clear link between disorders and upstream and downstream direction of creatine changes. Chronic mental stress conditions are thought to be connected with upstream regulation of cellular oxidative pathways, thus oxidizing various structural and active compounds. Oxidative stress also takes part in increase of permeability of blood brain barrier (BBB) that, in turn, makes it possible for a number of molecules to cross the BBB in both directions. Observations on long-term social isolation and circadian rhythm violation show a rising trend in brain creatine amount, while there was remarkable down-regulation in creatine synthesizing system, as the key-enzymes’ (AGAT and GAMT) activity was decreased. Investigations of BBB permeability for creatine under the stress conditions by mass-spectrometric analyses revealed no changes in creatine transport in the stress group, compared to the control. However, the activity of mitochondrial CK was reduced for about 25% and Vmax had fallen down in the stressed group, the Km was not drastically changed. To sum up, it could be supposed that the reason for the elevations of creatine levels in brain under the mental stress conditions could be stimulated by the activated oxidative stress that induces conformational changes in mitochondrial Creatine Kinase structure and decreasing the ability of enzyme to phosphorylate the creatine and as a result free creatine levels in brain are being arisen.
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Investigation on the formation of L-lysine based ternary deep eutectic solvents and their properties
Статья научная
DES is normally made up of two or more components, all of which are linked together by H-bonds. Abbott and colleagues were the ones who first proposed the concept of DES. As a mixture of two or more components forming an eutectic, the melting point of this eutectic mixture is lower than the individual components of that mixture. The usage of a totally enormous within the freezing point, as well as the capacity to exist in liquid state temperatures is below 150oC. DES, as a new type of green solvent, has certain - reputed properties, such as “high viscosity, high thermal stability and low vapor pressure”. L-lysine-glucose-malonic acid, L-lysine-fructose-malonic acid and L-lysine-fructose-ZnCl2 are three types of ternary deep eutectic solvents (TDESs) that we created in this study. They exist as a clear liquid for more than two weeks at room temperature. Its physical properties, such as “conductivity, density, viscosity pH and thermal properties as the thermal decomposition temperature” of these TDES were studied. The FTIR Spectra of those TDESs have been analyzed to apprehend the interaction of H bonds and three components.
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Ion partitioning and Mg2+/Na+ ratio under salt stress application in cotton
Статья научная
The response of five cotton (Gossypium hirsutum L.) varieties (A118), Aleppo33/1 (A33/1), Aleppo90 (A90), Raqqa5 (Raq5) and Deir-Ezzor22 (DE22) to salt stress (, 50, 100 and 200 NaCl) was studied in a pot experiment, in terms of their ionic distribution 56 days.and roots of DE22 exhibited the lowest Na+and Cl-and the highest leaf Mg2+contents conversely to A118. on this investigation, was no relationship between 2+content and salt tolerance of different cotton varieties. and root Mg2+/Na+ratios were decreased as salinity level increased for all tested varieties. In this respect, the value for the previous indicators was recorded in DE22 while the lowest one was recorded in A118. Thereby, among all tested varieties, DE22 variety relatively performed better under salinity compared to the other varieties.
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Статья научная
The desiccation tolerant cyanobacterium Lyngbya arboricola, isolated from bark surfaces of Mangifera indica, possessed up to four stable isoforms of catalase in addition to other antioxidative enzymes, for several years under a dry state. Purification of the two most persistent isoforms of catalase (Cat) has been undertaken by employing acetone precipitation, ethanol: chloroform treatment, gel filtration and ion exchange chromatography. The two isoforms of catalase remained almost unchanged on varying matric and osmotic hydration levels of mats of the cyanobacterium. The purification procedures resulted in a 1.3 % yield of purified single isoform (0.22 mg mL -1 protein) with 709 Units mg -1 specific activity and a purity index of 0.83. Five millimolar of dithiothreitol (DTT) was observed to be pertinent in maintaining the optimum redox state of the enzyme. The purification procedures additionally facilitated the simultaneous elimination and procurement of phycoerythrins (PE) and mycosporine-like amino acids (MAA). Each purified isoform gave a single band (~45kDa) upon SDS-PAGE and denaturing urea isoelectric focusing (IEF) depicted the presence of 2 subunits each of CatA and CatB. The monoisotopic mass and pI value of CatA and CatB as revealed by LC-MS analysis and internal amino acid sequencing was 78.96, 5.89 and 80.77, 5.92, respectively, showing resemblance with CatA of Erysiphe graminis subs. hordei and CatB of Ajellomyces capsulata. The heterotetrameric monofunctional catalase (~320 kDa), due to its stability in the form of resistance to ethanol: chloroform, its thermoalkaliphilic nature and the presence of innumerable hydrophobic amino acid residues (~40%), thus exhibited its potential for biotechnological applications.
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Isozyme analysis on different varieties of sugarcane
Статья научная
Isozymic and protein diversity among five sugarcane varieties viz., Co 6304, Co 85019, Co 8371, Co 89003 and Co 91010 were studied to understand the varietal interrelationship and to identify the biochemical marker for the disease resistance and stress tolerance. The standard technique of vertical gel electrophoresis PAGE was employed for size separation of isozymes. The gel was stained with different staining solutions for different isozyme systems viz. peroxidase, esterase, acid phosphatase, alkaline phosphatase and proteins. Rfvalues of the banding profiles, similarity index and variation between the varieties were analysed. Among the four enzyme systems, peroxidase profile reveals the difference between the disease resistant / susceptible and abiotic stress tolerant / non tolerant varieties. The two isoperoxidase bands with Rf values 0.62 and 0.66 showed their presence in disease resistant and abiotic tolerant varieties. The presence of two marker bands (0.62, 0.66) of resistant and stress tolerant varieties suggest that the variety Co 6304 may also be resistant to smut, wilt and moderately resistant to red rot and tolerant to drought.
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Lead (PB) heavy metal impacts in the green Ulva lactuca (Chlorophyceae) marine algae
Статья научная
Toxicity of different lead (Pb) (0, 2, 4 and 8 mg/L) concentrations in the green Ulva lactuca (Chlorophyta) marine algae at physiological level has been investigated 48 h after Pb treatment under laboratory conditions. Thalus algae damages followed Pb treatment as revealed by microscopy test showed that the 4 and 8 mg/L Pb caused morphological changes in cells viability; whereas, no effect observed at the lowest Pb applied concentration (2 mg/L). Data revealed that Pb stress caused reduction in most investigated physiological parameters i.e. Pigments content, osmotic potential and membrane stability index values. This decline in osmotic potential was significantly ( p ≤ 0.001) different. Whereas, estimated electric conductivity (EC) values increased significantly ( p ≤ 0.001) as applied Pb concentration increased. The current study allowed somewhat to highlight and better understanding Pb impacts in U. lactuca algae. Thereby, the studied algae could be used as a useful bioindicator in Pb polluted ecosystems.
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