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Preparation and characterization of renewable bio-polyol from the edible seed oil
Статья научная
Polyol is an organic compound containing multiple hydroxyl groups. This study looked at the possibility of using an edible oil extract from Salvia hispanica seeds as a sustainable source for polyols and, eventually, biodiesel or polyurethane. For this, a combination of hydrogen peroxide and acetic acid was used to create new polyol from the aforementioned oil in one-step synthesis. Standard techniques such as physicochemical analysis, phytochemical and basic radical identification, FTIR and NMR were used to characterize the polyol derivative that was extracted from the oil. Antimicrobial activity of both oil and polyol were tested against certain bacteria and fungi. Spectral analysis demonstrated the formation of polyol and this result indicated the possible of using Salvia hispanica polyol as a raw material for the preparation of bio-polymers.
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Статья научная
Seeds vigour and uniform germination across diverse environmental conditions is a primary objective in agriculture. Moisture and temperature are key factors affecting cotton seed quality during storage, where maintenance is rather difficult during long-term storage. To investigate the potential influence of UV radiation on enhancing cotton seed vigour after long-term storage, Deir Al-Zour 22 cotton variety was selected due to its reduced-vigour by time and its low germination rates. Germination rates of cotton seeds exposed to combined UV-A, B and C irradiation for different periods of time (4, 8, 12, and 16 min) were enhanced compared to non-irradiated seeds. Data showed improved growth of generated seedlings on PEG and NaCl supplemented media. Results showed no major changes on the expression of GA3ox1 gene, whereas, two stress-related genes DEH and VPP were temporarily activated after treatment with UV-irradiation supporting their function as scavenging and accumulating factors of ROS, a typical by-product of the photo-excitation under UV. Our results suggest the possibility of using combined UV-A, B and C radiation as a physical priming agent of cotton seeds to induce plant vigor and enhance germination under stress conditions without affecting normal growth.
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Статья научная
Radiolytically derived oligomers of sodium alginate are considered to act as signal molecules, affecting growth, development and defense mechanisms of plants through gene regulation. Since germination is a critical stage in the life cycle of plants, the objective of this study was to determine the effect of irradiated sodium alginate (ISA) on the characteristics of seed germination of Foeniculum vulgare Mill. Soaking the seeds in an aqueous solution of ISA showed significant improvement in various seed germination parameters, viz. germination, viability and relative water content of seed, nitrate reductase activity, protease activity, α and β amylase activities in germinated seedlings of fennel. GPC study revealed formation of lower molecular weight oligomer fractions in irradiated samples which could be responsible for plant growth promotion in the present work.
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Статья научная
Fusarium graminearum is one of the major global pathogens of cereals and is considered the main causal agent of Fusarium head blight (FHB) disease in wheat. F. graminearum leads to severe losses in grain yield and quality of wheat. In the present study, immature embryos of three Egyptian wheat cultivars (Giza 164, Sids 1 and Bani Suef 6) have been used to highlight the physiological changes in wheat plants in response to treatment with different concentrations of Fugal Culture Filtrate (5%, 10%, 20% and 40%). Samples were taken at 5, 10 and 15 days after inoculation. The three studied wheat cultivars exhibited different responses for inoculation with different concentrations of FCF. However, inoculation with 20% FCF significantly increased the activities of antioxidant enzymes (catalase, peroxidase and ascorbate peroxidase) in shoots of wheat cultivars compared with uninoculated ones. In addition, proline and secondary metabolites (total phenol and flavonoids) significantly increased throughout the experimental period. Our results also proved that in addition to the common protective mechanisms detected in all the infected cultivars there are cultivar - dependent physiological changes exhibited by wheat plant during abiotic stress.
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Статья научная
This study focuses on the search for a natural treatment to reduce lead toxicity in male Wistar rat by using the aquous extract of wheatgrass Triticum aestivum (WG). In addition to the control group (C), rats were exposed to a diet containing 600 mg Pb acetate/Kg diet (Pb), or combined with the WG at 9g/100g diet (Pb-WG) for a period of 6 weeks. Biochemical, reproductive and histological markers were evaluated. The results of haematological parameters show a very significant increase in white blood cells and lymphocytes with the group treated with the lead single. By contrast there was no difference was recorded between the treated group by Pb-WG and the control. A significant decrease in the red blood cells, the haemoglobin and haematocrit was recorded in the group treated with Pb alone. The tests of hormonal and biochemical parameters showed a decrease in the concentration of the hormone triiodothyronin (T3) and thyreostimulin (TSH) in the Pb group compared to the (control and Pb-WG). The serum testosterone concentration, urea, total cholesterol levels, the rate of TGP, TGO and creatinine were significantly increased in rats treated with (Pb) alone compared to (the control and Pb-bl group), while no change in glucose was shown. However, the (Pb-WG) group shows no change compared to the control. The level of calcium showed a significant decrease in the Pb group, and which returns to the normal state in group (Pb-WG) compared to the control. These results are confirmed by the study of histological sections. A morphological change represented by volume shrinkage of the vesicles. A return to the normal structure of follicles was observed in (Pb-WG) group. Histology study of the kidney, testis epididymis showed no change in (Pb-WG) compared to the control group. Contairement in the group exposed to lead (Pb) the parenchyma of the kidney shows dilated distal and proximal tubules causing renal tubular damage. The testes marked destruction and degeneration of germ cells and the light of some seminiferous tubules are empty. The study of the fertility parameters indicates a highly significant decrease in the concentration, the mobility of sperm counts among the treated group by Pb alone. The administration of the wheat grass has increased.
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Protective effect of d-ribose against inhibition of rats testes function at excessive exercise
Статья научная
An increasing number of research studies point to participation in endurance exercise training as having significant detrimental effects upon reproductive hormonal profiles in men. The means used for prevention and correction of fatigue are ineffective for sexual function recovery and have contraindications and numerous side effects. The search for substances effectively restoring body functions after overtraining and at the same time sparing the reproductive function, which have no contraindications precluding their long and frequent use, is an important trend of studies. One of the candidate substances is ribose used for correction of fatigue in athletes engaged in some sports. We studied the role of ribose deficit in metabolism of the testes under conditions of excessive exercise and the potentialities of ribose use for restoration of the endocrine function of these organs. 45 male Wistar rats weighing 240±20 g were used in this study. Animals were divided into 3 groups (n=15): control; excessive exercise; excessive exercise and received ribose treatment. Plasma concentrations of lactic, β-hydroxybutyric, uric acids, luteinizing hormone, total and free testosterone were measured by biochemical and ELISA methods. The superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase activities and uric acids, malondialdehyde, glutathione, ascorbic acids, testosterone levels were estimated in the testes sample. Acute disorders of purine metabolism develop in rat testes under conditions of excessive exercise. These disorders are characterized by enhanced catabolism and reduced reutilization of purine mononucleotides and activation of oxidative stress against the background of reduced activities of the pentose phosphate pathway and antioxidant system. Administration of D-ribose to rats subjected to excessive exercise improves purine reutilization, stimulates the pentose phosphate pathway work, inhibits oxidative stress of the testes, and saves the testicular endocrine function.
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Protective effect of ghrelin on isoniazid-induced liver injury in rat
Статья научная
Ghrelin (GHR) is a peptide that has protective effects on many tissues injury. It has anti-inflammatory and anti-oxidant effects. Isoniazid (INH) a widely used antituberculosis drug, has hepatotoxic side effect. The aim of this study was to evaluate the protective role of ghrelin in liver toxicity due to isoniazid. Eighteen male rats were used in this study and divided in to three groups. Including: control, isoniazid, isoniazid and ghrelin groups. Nitric oxide(NO), prostaglandin E2(PGE2), and hepatic enzymes, ALT(alanine aminotransferase), AST(aspartate aminotransferase), ALK(alkaline phosphatas), were assessed and histologic study of liver were performed as indicators of liver damage following isoniazid toxicity. Ghrelin significantly increased NO metabolites and decreased PGE2 level comparison with INH group, but had no significant change compared to the control group. This study showed that ghrelin administration inhibited liver injury in rats due to isoniazid toxicity. The liver protective role of ghrelin may be mediated at least in part by its anti-inflammatory effect.
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Protective effect of ghrelin on sodium valproate-induced liver injury in rat
Статья научная
Ghrelin is a peptide that has protective effects on many tissues injury. It has anti-inflammatory and anti-oxidant effects. Sodium valproate is widely used anticonvuisant and anti-depression drug with hepatotoxic side effects. The aim of this study was to evaluated the protective role of ghrelin in liver toxicity due to sodium valproate overdose. Eighteen rats were used in this study and divided in to three groups, containing: control, sodium valproate, and sodium valproate and ghrelin groups. Nitric oxide (NO), prostaglandin E2 (PGE2) and hepatic enzymes AST (aspartate aminotransferase) and ALT (alanine aminotransferase), were assessed and histologic study of liver were performed as indicators of liver damage following sodium valproate toxicity. This study showed the ghrelin decreased ALT and AST to the normal level. Our results show that ghrelin significantly increased NO metabolites and decreased PGE2 level comparison with sodium valproate group, but had no significant change compared to the control group. we showed that ghrelin administration inhibited liver injury in rats due to sodium valproate toxicity.
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Статья научная
Background Humic acid or chitosan has been shown to increase plant growth, yield and improving physiological processes in plant, but its roles on alleviating the harmful effect of cadmium on plant growth and some physiological processes in plants is very rare. Pot experiments were conducted to study the role of 100 and 200 mg/kg dry soil from either humic acid or chitosan on counteracted the harmful effects of cadmium levels (100 and 150 mg/kg dry soil) on radish plant growth and some physiological characters Results Cadmium at 100 and 150 mg kg-1 soil decreased significantly length, fresh and dry weights of shoot and root systems as well as leaf number per plant in both seasons. Chlorophyll, total sugars, nitrogen, phosphorus, potassium, relative water content, water deficit percentage and soluble proteins as well as total amino acids contents were also decreased. Meanwhile, cadmium concentration in plants was increased. On the other hand, application of chitosan or humic acid as soil addition at the concentration of 100 or 200 mg kg-1 increased all the above mentioned parameters and decreased cadmium concentrations in plant tissues. Chitosan at 200 mg kg-1 was the most effective than humic acid at both concentrations in counteracting the harmful effect of cadmium stress on radish plant growth. Conclusion In conclusion, both natural chelators, in particular, chitosan at 200 mg/kg dry soil can increase the capacity of radish plant to survive under cadmium stress due to chelating the Cd in the soil, and then reduced Cd bio-availability.
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Статья научная
Olive oil is beneficial effects are not only related to its high content of oleic acid, but also to the antioxidant potential of its polyphenols. In this study, we assess the effects of virgin olive oil on mercuric chloride induced oxidative damage in the liver of rats. Adult male Albinos Wistar rats randomly divided into four groups, were the first was served as a control, whereas the remaining groups respectively treated with: virgin olive oil (2ml/ kg b.w; by gavage), mercuric chloride (0.5 mg/kg body weight i.p) and combination of virgin olive oil and HgCl 2. Change in liver enzyme activities, thiobarbituric acid reactive substances (TBARS) level, antioxidants and reduced glutathione (GSH) contents were determined after 2 weeks of experimental period. Exposure of rats to mercuric chloride caused a significant increase the lipid peroxidation level along with corresponding decrease in the reduced glutathione and various antioxidant enzymes in liver. And increase in serum: glucose level, APL and transaminases activities and decreased in total protein and albumin levels. Furthermore, treatment with mercuric chloride caused a marked elevation of liver weight and decreased body weight. Supplementation of virgin olive oil resulted in decreased of lipid peroxidation level and in the serum: AST, ALT and APL activities were decreased along with increase in total protein, albumin and liver GSH levels. The activities of antioxidants enzymes: glutathione peroxidase (GSH-Px) and glutathione –S-transferase (GST) were also concomitantly restored to near normal level by virgin olive oil supplementation to mercuric chloride intoxicated rats. Liver histological studies have confirmed the changes observed in biochemical parameters and proved the beneficial role of virgin olive oil. The results clearly demonstrate that virgin olive oil treatment augments the antioxidants defense mechanism in mercuric chloride induced toxicity and provides evidence that it may have a therapeutic role in free radical mediated diseases.
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Статья научная
Background: Traditional medicine practitioners claimed that plant extracts are effective in the management of liver and kidney diseases in humans without scientific evidence. Thus, this study evaluated the protective effect of Alchornea cordifolia extract (ACE) and Byrsocarpus coccineus extract (BCE) against diclofenac sodium (DCF) induced oxidative stress and hepatorenal injuries in rats and compared their efficacies. Twenty four rats were divided into 4 groups with 6 rats per group. Normal saline was given to the rats in group 1 while those in groups 3 and 4 were treated with 250 mg/kg ACE and BCE respectively for 28 days by oral gavages. The rats in groups 2 to 4 were injected with 10 mg/kg DCF in the last 7 days of treatment. Blood was collected from rats, serum was separated from the blood and used for estimations of hepatorenal injury markers while the homogenized tissue supernatants were used for assays of oxidative stress markers. Results: There was a significant (p
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Protective role of sodium selenite on mercuric chloride induced oxidative and renal stress in rats
Статья научная
Backgroud: Reactive oxygen species are known to play a major role in mercuric chloride induced oxidative and renal stress. Sodium selenite as an exogenous source of selenium is used for endogenous selenoprotein synthesis to scavenge the free radicals. The study was designed to investigate the possible protective role of sodium selenite in mercuric chloride induced renal stress, by using biochemical approaches. Adult male Albinos Wistar rats were randomly divided into four groups. The first group was served as the control, the second group was given sodium selenite (0.25 mg/kg b.w), while the third group was given mercuric chloride (0.25 mg/kg), finally, the fourth group was given combined treatment of sodium selenite and mercuric chloride for 3weeks. Results: The effects of sodium selenite on mercuric chloride induced oxidative and renal stress were evaluated by serum creatinine, urea, uric acid, billirubin levels and LDH activity, kidney tissue lipid peroxidation, GSH levels, GSH-Px, GST and catalase activities and hematological parameters. Administration of mercuric chloride induced significant increase in serum: creatinine, urea, uric acid and billirubin concentration showing renal stress. Mercuric chloride also induced oxidative stress, as indicate by decreased kidney tissue of GSH level, GSH-Px, GST, and catalase activities along with increase the level of lipid peroxidation. Furthermore, treatment with mercuric chloride caused a marked elevation of kidney weight and decreased body weight and erythrocytes, hemoglobin, hematocrit levels. Sodium selenite treatment markedly reduced elevated serum: creatinine, urea, uric acid and billirubin levels, and LDH activity and conteracted the deterious effects of mercuric chloride on oxidative stress markers and hematological parameters and atteneuated histopathological changes caused by HgCl 2 in kidney. Conclusion: Our results indicate that sodium selenite could have a beneficial role against mercuric chloride induced nephrotoxicity and oxidative stress in rat.
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Protein profile analysis by SDS-page in different elicited edible sprouts
Статья научная
Sprouts are the low processed food, having the high nutritional value and health promoting components. Various studies reported that the bioactive composition of various sprouts enhanced by elicitation with different elicitors. In the present investigation, the protein profiling by SDS-PAGE of different sprouts (mungbean, chickpea, broccoli and knolkhol) which were elicited with different elicitor viz salicylic acid (SA), methyl salicylate (MeSA) and neem leaf extract (NE) were performed. Results of the study showed that in all the sprouting seeds variety of bands were obsereved ranging from 8.31 to 112 kDa. The significant increase in intensity of protein bands was observed in 1% MeSA and 20 mM SA treatments in mung bean and chickpea sprouts, whereas, in the case of broccoli and knolkhol sprouts, only SA (200 µM) treated sprouts showed increased protein band intensity. This quantitative increase may be due to an increase in the synthesis of some proteins during elicitation. Elicitation can enhance the polyphenolic such as phenolic, flavanoids, vitamins, amino acids and glucosinolates content in sprouts by regulating the expression level of gene which are responsible for the synthesis of various enzymes which are required for the production of secondary metabolites. Moreover, our previous studies also reported that elicitation with different elicitors effectively increases the biological activities of sprouts. Therefore, from this study it may be concluded that the elicitation is an effective approach for the enhancement of nutritional and bioactive composition of seed.
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Purification and Biochemical Properties of Carboxylesterase from Saga Seeds (Adenanthera pavonina)
Статья научная
Carboxyl esterase (E.C.No.3.1.1.1) was partially purified from Adenanthera pavonina (Saga) using ammonium sulfate fractionation (0-60%) and DEAE (diethyl aminoethyl) ion exchange chromatography, the purified enzyme was characterized. One major saga-esterase was identified with Fold purification of 29. Molecular weight of the Ap-esterase was determined using Sephadex G-25 gel filtration and SDS-PAGE (Sodium dodecyl sulfate polyacryamide gel electrophoresis) which was found to be 26.0 k Da. Optimal activity of the saga-esterase occurred when the pH 7.0 at a temperature of 55°C. The activation energy for the hydrolysis of α-naphthyl acetate was determined to be 1.10 kcal/ mol. The Michaelis Menton constant (Km) and Vmax of the saga-esterase was 0.4µmoles and 105 IU respectively. In addition, the isoelectric point is at pH > 9 and immuno-blot using polyclonal antibodies showed that the saga-esterase was widely distributed in seeds but not in leaves. The saga-esterase inhibited by organophosphate and carbamate pesticides, which can be substituted for acetylcholinesterase.
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Статья научная
In order to identify the components of high temperature response in French bean, three heat shock proteins induced under high temperature were purified to homogeneity by Carboxy methyl cellulose and sephadex G-100 chromatography followed by preparative SDS-PAGE. Two of these, Hsp1 and Hsp3 were further characterized by immuno-detection with polyclonal antibodies. Hsp3 exhibited ATPase and chaperone activity with malate dehydrogenase and citrate synthase. Partial cDNA for Hsp3 synthesized using the primer derived from amino-terminal sequence was cloned and expressed in Escherichia coli. The recombinant protein possesses ATPase activity, and showed thermal protection at 50°C in Escherichia coli. The translated partial cDNA showed homology with stress induced proteins including ATPases from higher plants. These results supported the fact that French bean response to high temperature stress involves Hsps as one of the principal components.
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Qualitative Phytochemical Screening of Ethnomedicinal Plants Used by Paraja Tribe of Koraput, India
Статья научная
The present study reports the qualitative phytochemical screening of selected ethnomedicinal plants used by Paraja tribe of Koraput which are known to exhibit medicinal properties reported earlier in our laboratory. The plants were subjected for phytochemical screening to determine the presence of secondary metabolites, which showed therapeutic effects as claimed by the tribal people. Qualitative analysis of alkaloids, flavonoids, tannins, saponins, stereoid, terpenoid, glycosides, anthraquinons and phenol tests were carried out for these selected plants by using different extraction (methanol, acetone and water extraction). The phenol, flavonoid, alkaloid, saponin, tannin, and steroids are major pharmaceutical parameters that are responsible for ethnomedicinal values were present in varying proportions. Based on the results, these plant species also contain significant amounts of phytoconstituents that can be exploited as a potential source for herbal remedy for various diseases. Further studies are necessary to find its different bioactivity which can give fruitful results in phytopharmaceuticals.
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Qualitative and quantitative phytochemical screening of Tinospora cordifolia (Willd.)
Статья научная
Background: T. cordifolia contains a lot of secondary metabolites which are produced from various parts of the plant. They have their specificity in many diseases to cure. By considering the importance of T. cordifolia as medicinal plant to investigate the phytochemical properties. Results: The preliminary phytochemical study was carried out to identify the presence of different phytochemicals and the results revealed the presence of Carbohydrates, Glycosides, Proteins, amino acids, Alkaloids, Flavonoids, Phenolic compounds, Tannins, Saponins, steroids and triterpenoids. When we compare the presence of phytocompounds in different solvents, methanol and aqueous extracts revealed the presence of more phytoconstituents followed by petroleum ether and chloroform solvents. The quantitative analysis of phytochemicals such as Flavonoids, phenols, alkaloids, tannins and saponins were also estimated from methanol extract by various methods. Total phenolic content of methanolic extract of the T. cordifolia was found to be 6.53mg/g. Total flavonoid content was found to be 4.07mg/g, alkaloid content was 5.62mg/g and the tannin content was found to be 5.83 mg/g. Saponin content was 5.02mg/g. The results revealed that the phenol content was more in stem extract of T. cordifolia and it was followed by tannins, alkaloids, flavonoids, and saponins. FT-IR analysis was performed to identify the various functional groups of the biomolecules found in the methanolic stem extract of the plant. Conclusions: The results of present study revealed the presence of medicinally important constituents in Tinospora cordifolia . Traditional medicine practice is recommended strongly that further work should be carried out to isolate, purify and characterize the bioactive compounds responsible for the activity of these plants.
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Quantitative Evaluation of Rice (Oryza sativa L.) Under High Temperature Stress
Статья научная
Rice (Oryza sativa L.) is world’s second highest produced grain crop after maize. It is India’s preeminent staple crop among other cereal crops. It is a major source of food after wheat. However, it is highly sensitive towards high temperature during flowering and early grain filling stages hence, threaten crop productivity. The various anthropogenic activities increase the global temperature at an alarming rate which causes negative impact on agriculture in terms of reduction in growth and yield. With increasing population and changes in global climate, the current rice production will be inadequate to meet the future demand very soon. Therefore, use of temperature resistant variety can be a better alternative. The experiment was carried out with thirty two genotypes of rice to evaluate genotype for growth and yield attributing traits under high temperature conditions. The genotypes under investigation were observed at flowering and maturity stage after the treatment of high temperature. Among all the genotypes, IET 23947, Luit and IR-82310-B-B67-2 were found most tolerant to high temperature as they showed significant enhancement in grain yield (70.95%), 1000 grain weight (36.72%) and harvest index (43.58%). Therefore, these three potential genotypes can be exploited for further studies with respect to high temperature stress (heat stress) to obtain the best heat resistant rice genotype with high production and improvement of rice genotype for heat resistance.
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Quantitative analysis of physicochemical and phytochemical constituents medicinal herbs
Статья научная
The present investigation has been carried out the Stastical Analysis of physicochemicaland phytochemical constituents medicinal herbs such as flower of Calendula officinalis Linn, leaves of Lantana camara Linn,aerial part of Oldenlantia herbacea L and root of Peristrophe paniculata Forssk.In physicochemical analysis, parameters such as foreign matter, loss drying,total ash,water and acid soluble ash, water and solvent extractive value of plant were determined.And also the quantitative estimation of phytochemical constituents like alkaloids, tannin, terpenoids, saponin, carbohydrates and protein contents of Calendula officinalis L., Lantana camara L., Oldenlandia herbacea (L.) Roxb., and Peristrophe paniculata Forssk. was undertaken as per standardized procedure.Result showed that Loss on drying turned out to be 1.70% in Calendula officinalis L.,1.61 % in Lantana camara L.,1.45% in Peristrophe paniculata Forssk. and 1.31% in Oldenlandia herbacea Roxb. Foreign matters were found to be 2.36% ( Calendula officinalis L.), 2.67% ( Lantana camara L.), 4.03% ( Peristrophe paniculata Forssk.) and 3.73% in Oldenlandia herbacea (L.) Roxb and the toal ash was found to be relatively 9.46% in Calendula officinalis L. followed by 10.62 % in Lantana camara L., 9.53% in Peristrophe paniculata Forssk. and 8.34% in Oldenlandia herbacea Roxb. The water solubility of all the selected plants 21.74% ( C. officinalis ), 22.43% ( L. camara ), 22.58% ( P. paniculata ) and 22.68% ( O. herbacea ) were found to be higher compared to ethanol and other solvents. The phenol content was higher in Lantana camara L. (15.95mg) followed by Calendula officinalis L. (12.57mg), Peristrophe paniculata Forssk. (10.63mg) and Oldenlandia herbacea (L.) Roxb. (3.70mg). The high content of alkaloid was recorded in Calendula officinalis L.(12.57mg) and low content in the Peristrophe paniculata Forssk. (0.39mg). The total tannin content of Calendula officinalis L. ( 78.93µg) was found to be higher than that of other plants.The total flavonoid content of Calendula officinalis L. was 21.51mg followed by 16.44mg for Lantana camara L., 21.29 mg for Peristrophe paniculata Forssk. and 18.43 mg for Oldenlandia herbacea (L.) Roxb. Oldenlandia herbacea (L.) Roxb. contains higher concentration of terpenoids (11.65mg/g) compared than that of other three plants. Among the four plants, the levels of carbohydrate and protein content were found to be higher in Calendula officinalis L.(61mg; 5.20mg) respectively. From th results it was concluded that this study will be helpful in standardization for quality, purity and sample identification.
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Статья научная
Calanthe masuca (D.Don) Lindl plant leaves have medicinal properties. It is an endangered and terrestrial orchid. They are distributed in evergreen forest; plant collection is very difficult for researches. That’s why very rare because of group of plants not widely represent in the forest. The present study has aimed to evaluate the phytochemical analysis using three different solvents Methanol, Aqueous and Petroleum Ether. The secondary metabolites have estimated into phenols, flavonoids, alkaloids, tannins, saponins, steroids and terpenoids. The result showed that the methanol leaf extract shows as a high concentration. Aqueous extract showed moderate concentration and Petroleum ether extract showed low concentration. Methanol extract contained secondary metabolites such as the total phenol (31.50±0.22 mg/g) and tannins (24.60±0.02 mg/g) as mg, alkaloids (54.40±0.33 mg/g) as mg, flavonoids (73.20±0.62 mg/g) as mg, saponins (35.00±0.05 mg/g), Steroid (60.95±0.57 mg/g) and Terpenoids (14.00±0.26 mg/g). The Calanthe masuca is a medicinal plant based on the secondary metabolites presence of phenols, flavonoids, alkaloids, tannins, saponins, steroids and terpenoids. They are used as a source of potentials therapeutic medicinal values and curing various infectious diseases.
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