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Silicon root irrigation enhances barley resistance to Fusarium head blight
Статья научная
Silicon (Si) is recognized for its protective role in decreasing disease damage when absorbed by barley plants and has been proposed as a possible solution against Fusarium head blight, associated with devastating agronomic effects on overall yield and grain quality. However, root treatment of exogenous Si irrigating to enhance host resistance to Fusarium infection is unknown. For this purpose, a series of greenhouse experiments was conducted to examine the effects of Si irrigation at 1.7 mM to roots on pathogen development in barley heads. Two barley cultivars with contrasting FHB resistance (moderately resistant Arabi Aswad, AS, and moderately susceptible Arabi Abiad, AB) and infected with four Fusarium species with diverse pathogenicity were used. The quantification of the disease was through the determination of the disease incidence (DI, Type I resistance), disease severity (DS, Type II) and area under disease progress curve (AUDPC) calculated on the basis of DI and DS. Si absorption in barley enhanced the defense system in head tissues to pathogen invasion; FHB developed more severely on AS and AB plants grown without Si irrigation than on plants supplied with Si. Barley plants treated with exogenous Si irrigating were associated with a reduction of up to 19.3%, 19.8%, 18.7%, and 20.0%, respectively, in DI, DS and AUDPC calculated on the basis of DI and DS. Si contributed to the reduction of FHB in barley, especially for the moderately resistant cultivar; however, Si reduced the intensity of FHB in AB to a level comparable with AS. Importantly, Si treatment at 1.7 mM decreased disease damage FHB in previous bio-trials conducted on AS and AB under in vitro and field environments, showing that Si enhanced the expression of resistance to FHB infection in seedlings and adult barley plants. Taken together, the link of Si and host resistance provided a greater decrease in head blight in which both cultivars had augmented performances upon exogenous Si irrigating to roots; highlighting that Si is a potential safe and efficient policy to defend barley when invaded by Fusarium .
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Статья научная
Among the great diverse of Baikal fauna of amphipods (more than 350 species and subspecies) Gmelinoides fasciatus (Stebbing 1899) takes the special place. It is characterized by a high morphological variability and wide thermal-adaptive possibilities. By common opinion G. fasciatus is a thermophilic relict segregated from other Baikal temperature sensitive amphipods' fauna. In present study we tested hypothesis of segregated status of G. fasciatus among some Baikal and some Palearctic amphipods using of heat-shock proteins' (HSP) molecular weight as comparative biomarkers. We used heat-shock proteins (HSP) from two families: HSP70 and small HSP (sHSP) immunochemically related to α-crystalline. 15 species of Baikal amphipods from different genera and families and 2 Palearctic species from genus Gammarus were tested. It was shown, that molecular weights of HSP70 were the same in all investigated species and corresponds close to 70 kD. In the contrast, the molecular weights of sHSP in G. fasciatus was 37 kD and differed from all other species, in which molecular weights of sHSP were 35 kD. In sum, this study showed that sHSPs molecular weights may relate to evolutional differences between the close related species. Additionally, obtained data can be taken as new indication of segregated status of thermophilic relict G. fasciatus which linked with its phylogenetic history in Lake Baikal.
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Статья научная
The exogenous application of plant derived smoke solution through seed pre treatment is consider to create tolerance in the plant against salinity, for this purpose different dilution of plant derived smoke solution as 1:5000 Buhania, 1:1000 Buhania, 1:1000 Cymbopogon, 1:500 Cymbopogon were used against 0 mM, 50, 100 and 150mM NaCl solution in the medium. The effect was observed on total proline accumulation, heavy metals uptake, photosynthetic pigments and protein poly peptide bands intensity in two rice varieties as Basmati 385 (B-385) and Shaheen Basmati (S. Basmati). Proline concentration increases while chlorophyll “a” chlorophyll “b” and carotene level decreases with increasing salinity. On other hand zinc concentration increases while cadmium and lead concentration decrease in the crop under saline conditions. Intensity of protein polypeptides bands decreases gradually with increasing salinity level but plants from the seeds soaked with smoke solution alleviate the drastic affect of salinity, and intensity of bands is quite good by comparing with non primed seeds. It is concluded that seed priming with plant derived smoke solution show beneficial effect on crop to protect them from salinity.
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Статья научная
The present study was conducted to investigate the impact of irrigation with industrial wastewater on soil and plant. For these purpose turnip and lettuce plants were cultivated in soil irrigated with wastewater then heavy metals content of the soil, plant growth, yield and the subsequent changes in biochemical constituents of plant were examined. Irrigation with wastewater was found to load the soil with heavy metals (Pb, Co, Ni and Cd) that were not detected in soil before irrigation. The magnitude of Cd in soils after irrigation with industrial wastewater exceeds the maximum allowable limit (3 mg Kg -1). Both turnip and lettuce exhibited significant decreases in leaf area, fresh weight and dry weight of shoots and roots as well as all the measured yield components in response to wastewater irrigation. The magnitude of decrease was positively correlated with the amounts of heavy metals detected in the soil and the inhibitory effect on turnip was much more pronounced than in lettuce. Furthermore, heavy metals accumulation in soil resulted in an oxidative damage to turnip and lettuce as indicated by the significant increase in lipid peroxidation and H 2O 2 levels in both plants comparing to control values. The significant increases in putrescine in lettuce and turnip shoots and roots and spermidine in lettuce roots as well as total phenolics and flavonoids in plants cultivated in soil enriched with heavy metals are believed to be defense mechanisms in turnip and lettuce plants to counteract the oxidative stress resulted from heavy metals contamination generated from irrigation with wastewater.
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Статья научная
In this monograph, based on many years of research by the author, systematization and generalization of soil and cartographic materials of the Siberian Institute of the Siberian Branch of the Russian Academy of Sciences, as well as the use of a large number of literary and fund sources, the characteristics of soil formation and soil cover in the Irkutsk region are given. It is shown that, due to the great extent of the territory of the region from north to south and from west to east, the heterogeneity of the relief, geological structure, climatic conditions and vegetation, the soil cover is characterized by a complex structure and a wide variety of soil types. Based on the latest research and modern ideas on the genesis of soils, a systematic description of the soils and their diagnostics is given, specific features of the temperature and water regimes of seasonally frozen soils, the structure of the soil profile, physical and chemical properties are reported, which inform the regional features of the soils forming here, determining the fertility of soils and Ways of their rational use in agricultural production. A new scheme for soil-geographical zoning of the territory of the Irkutsk region was developed, which was used as the basis for the soil map of the Irkutsk region at a scale of 1:1 500 000, published by the USSR in 1988. Areas of soil zones, sub-zones and districts were determined and the degree of their agricultural use was indicated. The calculations of the areas of the main types of soils along the soil zones, subareas and districts used in agriculture are given. The data contained in the monograph make it possible to assess the potential for further agricultural development of soils both in the southern regions of the region and in the north - in areas of new industrial construction and in the zone of the western section of the BAM. The work can serve as an explanatory note to the soil map of the Irkutsk region at a scale of 1: 1 500 000, published in 1988. This is the 3-d part of Chapter 4 of the monograph.
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The main objective of this study was to establish the stable and efficient protocol for somatic embryogenesis and plantlet regeneration under NaCl-salt stress conditions in traditional Indian red rice (cv. Poongar) using mature caryopsis as explant and also to assess the genetic fidelity in regenerated plantlets. High frequency (98.88±1.33%) of callogenesis was recorded in MS (Murashige and Skoog, 1962) medium fortified with 10µM of 2,4-D (2,4-Dichlorophenoxyacetic acid) while maximum percentage (95.0±3.9%) of somatic embryogenesis and also maximum number (47.3±3.9) of somatic embryo per callus was recorded in presence of 5µM of 2,4-D alone. Furthermore, maximum frequency (85.33±2.02%) of germination of somatic embryos into plantlets was found in MS medium supplemented with 2.0mg/L of IAA (Indole-3-acetic acid), 3.0mg/L of BAP (6-Benzylaminopurine) and 1.0mg/L of Kn (Kinetin). During the salinity stress treatments, various concentrations (10mM, 25mM, 50mM and 100mM) of NaCl were used in this study in order to induce somatic embryogenesis and plantlets regeneration. Significantly, 100mM of NaCl shows the strong inhibitions for callus induction (26.67±0.63%)and somatic embryogenesis (31.3±1.8%),moreover, weight of the callus was also found to get decreased (94±0.5mg) than control (156±4.3mg) treatment. Interestingly, the highest concentration 150mM of NaCl was proved to be completely inhibitory and function show_abstract() { $('#abstract1').hide(); $('#abstract2').show(); $('#abstract_expand').hide(); }
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Present study aims to establish somatic embryogenesis and plantlet regeneration from cotyledon culture in peanut ( Arachis hypogaea L. cv. TMV13) under NaCl-stress conditions. Cotyledon tissue was found to be efficient for somatic embryogenesis in MS medium fortified with 10mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D). Moreover, in order to achieve salt-tolerant regenerants in peanut, cotyledons were inoculated on MS-medium supplemented with various concentrations of NaCl-salt in presence of 2,4-D (10mg/L). Results indicate that with the increase in NaCl-concentrations in medium, frequency of embryogenic callus formation gradually declines and obtained as minimum (17.1±0.16%) with high concentration (150mM) of NaCl-salt while NaCl (200mM) was proved to be lethal. Further, salt-tolerant embryogenic callus was transferred to medium added with (0.5mg/L, 1.0mg/L, 1.5mg/L, and 2.0mg/L) of BAP in combination with (1.5mg/L) of IAA and NaCl (100mM) for the germination of somatic embryos followed by shoot regeneration. Significantly, the maximum frequency (70.2±0.59%) of shoot regeneration was obtained on medium containing BAP (1.5mg/L), IAA (1.5mg/L) and NaCl (100mM). Moreover, salt-tolerant regenerated shoots were further transferred to medium containing kinetin (1.0mg/L, 2.0mg/L, 3.0mg/L, and 5.0mg/L) along with NAA (1.5mg/L) and NaCl (100mM) for root initiation. The high frequency (51±0.7%) of root regeneration was observed on ½ MS medium containing kinetin (2.0mg/L), NAA (1.5mg/L), and NaCl (100mM). Interestingly, rooting medium fortified with NaCl (100mM) was also proved to be effective for precocious induction of in vitro flowering (26.66±0.12%) in the regenerated plantlets. The regenerated plantlets were further transferred to plastic cup soil and acclimatized under greenhouse conditions. The result indicated, salt-tolerant peanut cotyledon culture also shows In Vitro plantlet regeneration and flower formation.
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Some desacarisation-associated sanogenetic mechanisms in chickens after dermanyssosis
Статья научная
The paper presents some natural supramolecular sanogenetic mechanisms that occur in the body of chickens after dermanyssosis with the participation of stress-associated hormones, cortisol and triiodothyronine, during desacarisation. This study contains a description of physiological and biochemical bases of recovery of the body after an extreme environmental factor (haematophagous ectoparasite Dermanyssus gallinae ). Particular scientific interest is in studying changes in the dynamics of morphophysiological and biophysiochemical blood parameters in birds with eliminated stress factor of extreme strength, an aggressive haematophagous ectoparasite, and a concurrent desacarisation of the poultry building with a synthetic pyrethroid-based drug. At the same time, partially normalized key parameters of endocrine and metabolic homeostasis was detected in the birds after dermanyssosis, which occurred due to activated natural sanogenetic mechanisms, and compensatory and adaptive reactions. Significant changes were detected in the hormonal state in the chickens from the experimental group that resulted in the decreased cortisol-producing function of the adrenal glands and the increased thyroid gland functional activity. The latter is, in our opinion, the most important sanogenetic mechanism that ensures maintenance of the antioxidant status, which is especially necessary when the body recovers after an extreme environmental factor. Along with this, a natural recovery of the RBC elements as well as immunity was observed. At the same time, decreased intensity of lipid peroxidation processes in the experimental chickens predetermines maintaining the integrity of these cells.
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Sorbic and benzoic acids affect adrenal morphology under chronic stress in male rats
Статья научная
Background: Chronic stress significantly impacts endocrine function, especially in the adrenal glands, which produce hormones essential for stress adaptation. Food preservatives such as sorbic and benzoic acids are widely used in the food industry, yet their long-term effects on the endocrine system under stressful conditions remain poorly understood. This study aimed to determine whether combined chronic stress and high-dose preservatives induce more pronounced structural changes in the adrenal glands of male rats than either factor alone.
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Stress induced alterations in pre-pubertal ovarian follicular development in rat
Статья научная
The objective of the study was to find out whether stress experienced during neo-natal period alters the timing of formation of pre-antral and antral follicles and if so, whether pre-treatment with CRH receptor antagonist prevents these effects in rats. New born rat pups (n= 15) were exposed to maternal separation (6 hours/ day) from post-natal day (PND) 1 to 7 and were killed on PND 8, 11 and 15. The time of exposure was randomly changed every day during light phase (7Am to 7Pm) of the day to avoid habituation. There was a significant increase in serum corticosterone levels on PND 8 and 11 in stress group rats compared to controls indicating stress response in these pups. The ovary of both control and stressed rats contained oocytes and primary follicles on PND 8 and 11 and in showed progress of follicular development upto to pre-antral and early antral follicle formation on PND 11 and 15. However, mean number of healthy oocytes and all categories of follicles at all ages studied were significantly lower in stressed rats compared to controls. Concomitant with these changes, number of atreatic follicles showed an increase over control values in stressed rats. The increase in atresia of follicles was due to apoptosis as shown by increase in the percentage of granulosa cells showing TUNEL positive staining and caspase 3 activity. On the other hand, pre-treatment with CRH- receptor antagonist (CRH 9-41) 2ng/ 0.1 ml/ rat prior to undergoing stress regime on PND 1 to 7, prevented alterations in pre- pubertal follicular development thereby indicating that the ovarian changes were due to effects of stress induced activation of HPA axis. The results indicate that, stress during neonatal phase, though does not affect timing of formation of pre-antral and antral follicles, it does enhance atresia of follicles of all categories, including follicular reserve, which may affect the reproductive potential of adults. The results, for the first time reveal that CRF receptor antagonist prevents pre-pubertal ovarian stress response.
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Статья научная
The stress induced effect of pulp and papermill effluent in the fish, Oreochromis mossambicus was investigated in the present study. Pulp and papermill effluent discharged from Hindustan News Print LTD, Peruva, Kottayam (Dist), Kerala was collected from the discharging point and used for the study. The adult Oreochromis mossambicus of size 15 ± 2 g were exposed to a sublethal concentrations (1/5th and 1/10th LC50 value) of the effluent for a period of 10, 20 and 30 days. biochemical parameters in the liver such as carbohydrate, protein, lipid and enzymes were analysed. Energy yielding nutrients such as carbohydrates, proteins and lipids were decreased tremendously for the study as the concentration and duration of the effluent exposure increased. Increased activity of enzymes such as GOT, GPT and LDH showed that the fish was under stress when exposed to pulp and papermill effluent. Fish exposed to papermill effluent showed reduced activity of ACP and ALP as the duration of exposure and concentration increased.
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Stress related variations in serum vitamin e and c levels of Murrah buffaloes
Статья научная
He study was designed to determine stress related variations in endogenous vitamin E and C levels of Murrah buffaloes. For this purpose, four hundred and fifty healthy adult female Murrah buffaloes between 4 and 12 years of age were sampled to harvest the sera during adverse ambiences viz. moderate, extreme hot and cold ambiences. Animals were broadly divided into non-pregnant milch, pregnant milch, pregnant dry, primipara and multipara. The mean values (µmol L-1) of serum vitamin E and C were 4.31±0.03 and 23.34±0.33, respectively during moderate ambience. The mean values of both the vitamin E and C depressed significantly (p≤0.05) during hot and cold ambiences as compared to moderate ambience. It was observed that decline in each value during hot ambience was greater than that of respective cold ambience. A significant (p≤0.05) variation was observed in the mean values of vitamin E and C in each ambience in the animals of all physiological states. The mean values of both the vitamins of non pregnant milch animals were highest (p≤0.05) whereas they were lowest(p≤0.05) in pregnant dry animals. It could be concluded that extreme ambiences produced oxidative stress in the buffaloes of all physiological states. The depressed levels of endogenous vitamin E and C in the serum showed their depletion in the body probably to combat free radical scavengers. Vitamin E and C should be supplemented to protect the animals from oxidative stress.
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Статья научная
Gymnema sylvestre is a perennial woody climber belonging to the family Apocynaceae. The leaves and roots of the plant have medicinal properties and are used in the Ayurveda system of medicine to treat various diseases. It is an important medicinal plant mainly for its antidiabetic properties and also it possess anti-inflammatory, anti-microbial and anti-oxidant activities. In this study the effect of stress signals in the pigment production and anti-oxidant potential assessed. The experiment was conducted in a poly house with twelve replications of each treatment. The different stress signals were Zinc sulphate, Sodium nitroprusside, Salicylic acid, Jasmonic acid and Squalene. The present study reveals an idea about the effect of stress signals in antioxidant activity and pigment production. The fresh leaf samples of treated plants were collected for pigment estimation. The leaf samples of treated plants were collected, dried and extracted in methanol and this methanolic extracts were used for analysis of anti-oxidant activity. A variation in both the parameters observed in different treatments.
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Strictly NO 3nutrition alleviates iron deficiency chlorosis in Arabidopsis thaliana plants
Статья научная
The effects of NO 3- nutrition on iron deficiency responses were investigated in Arabidopsis thaliana. Plants were grown with or without 5 µM Fe, and with NO 3- alone or a mixture of NO 3- and NH 4+. The results indicated that, NO 3- nutrition induced higher dry matter production, regardless the Fe concentration. Fe deficiency reduced growth activity, photosynthetic pigment concentration and Fe content of plants, whatever the N forms. This decrease was more pronounced in plants grown with mixed N source; those plants presented the highest EL and MDA and anthocyanin contents compared to plants grown under Fe sufficient conditions. In iron free-solutions, with NO 3- as the sole nitrogen source, enhanced FC-R activity in the roots was observed. However, in the presence of NH 4+, plants displayed some decrease in in FC-R and PEPC activities. The presence of NH 4+ modified typical Fe stress responses in Arabidopsis thaliana plants.
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Structural and Functional Characterisation of the Chitinase Gene in Chickpea under Aluminum Stress
Статья научная
Aluminum (Alі⁺) toxicity in acidic soils severely limits chickpea (Cicer arietinum L.) productivity by impairing root growth and suppressing defence responses. Chitinases are well studied for antifungal activity, but their role under aluminum stress is largely unknown. We present the first structural and functional characterization of chickpea Chitinase 10 (XP_004494478.1) under Alі⁺ stress using in silico approaches. Multiple sequence alignment and phylogenetic analysis revealed strong conservation and close relatedness to legumes such as Medicago truncatula and Cajanus cajan. Conserved domain mapping and 3D modeling confirmed a stable GH19 catalytic fold. The protein–protein interaction analysis demonstrated that Chitinase 10 is acting as a central hub in the stress responses, while the molecular docking analysis demonstrated that Feі⁺ attaches strongly to a distant site, while Alі⁺ attached comparatively weakly but destructively to the catalytic site near Lys46, Glu228 and Asp231, inhibiting the activity of the enzyme. Our findings reveal that aluminum disrupts the function of Chitinase 10 by disrupting the catalytic site, disrupting its antifungal mode of action. This mechanistic connection indicates that aluminum toxicity disrupts plant defence and provides structural basis to inform breeding or biotechnological applications to improve aluminum tolerance in chickpea.
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Structural and functional alteration of photosynthetic apparatus in rice under submergence
Статья научная
Submergence induced alteration of photo-system II (PS II) structure and function was probed using fast O-J-I-P chlorophyll a fluorescence transient and CO2 photo-assimilation rate. Submergence resulted in an inhibition of CO2 photo-assimilation rate and reduction in leaf chlorophyll content in rice but the decrease was more in submergence susceptible (IR 42) and avoiding type (Sabita) cultivars compared to the tolerant (FR 13A) one. Quantification of the chlorophyll a fluorescence transients (JIP-test) revealed large cultivar differences in the response of PS II to submergence. The kinetics of chlorophyll a fluorescence transient showed complex changes in the magnitude and rise of O-J, J-I and I-P phases of fluorescence rise. Due to submergence both donor and acceptor sides of PS II were damaged and electron transport perturbed, which resulted in the fall of CO2 photo-assimilation rate. Submergence also affected overall grouping probability (PG) or energetic connectivity between PS II antennae. The fall of PG was greater in submergence susceptible and avoiding types of cultivar compared to the tolerant one. These indicate that chlorophyll fluorescence can be used to know the sensitivity of rice to submergence stress.
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Role of structural carbohydrate content and lignifications in rice shoot were studied in three indica rice ( Oryza sativa L.) cultivars namely FR13A (tolerant to submergence), IR42 (susceptible to submergence) and Sabita (adapted to medium depth, 0-50 cm stagnant flooding), varying degrees of tolerance to submergence under control and 8 days after submergence conditions. During submergence, Sabita and IR 42 accelerated the rate of stem and leaf elongation more than that of FR 13A. Submergence significantly reduces the cellulose and hemicelluloses content. The decrease was more pronounced in susceptible rice cultivar than that of tolerant rice cultivar. Lignifications’ was monitored by measuring the content of lignin and the activities of two enzymes of the lignin biosynthetic pathway, coniferyl alcohol dehydrogenase (CAD) and phenylalanine ammonia-lyase (PAL) in rice shoots. Lignin content and PAL and CAD activity was more in susceptible cv. both under control and 8d after submergence. In conclusion submergence induced elongation of rice shoot might decreased the structural carbohydrate level as our experiment showed a significant negative correlation of cellulose and hemicelluloses with plant height but also positively associated with plant survival under submergence. The content of lignin and activities of CAD and PAL showed negative association with shoot elongation, yet the association of these parameters with survival was non-significant.
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Studies on developmental variation of isoperoxidase and protein profile of Zea mays L
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The present study was aimed to produce the isoperoxidase and protein marker for the maize cultivars from India and to find the isoperoxidase and protein expression on the various developmental stages. The leaf samples of Zea mays L. were harvested on 3rd, 7th, 11th, 15th, 19th, 23rd and 27th d for electrophoretic analysis of isoperoxidase and protein. The total protein and isoperoxidase were isolated using the standard procedure described by Sadasivam and Manickam. Isozyme and protein separation was carried out using 10% non-denaturing polyacrylamide gel electrophoresis. The banding pattern suggested several similarities as well as presence of unique isoperoxidase and protein in each stage. These studies will be useful for the identification of the major proteins present at different developmental stages of Z. mays.
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The fall army worm (FAW) ( Spodoptera frugiperda ) is a polyphagous pest which causes damage to commercially important cultivated crops such as maize, rice, sorghum, cotton and also different vegetable crops. Carboxyl esterases (CarE, EC.3.1.1.1) or esterases are enzymes in the carboxyl / cholinesterase gene family that catalyze the hydrolysis of carboxyl esters. The carboxylesterases enzyme are the detoxification enzymes in FAW. Therefore, the inhibition of carboxylesterases from FAW would help in pest management. In this scenario, Isolation, purification and inhibition studies were carried out on the midgut carboxylesterase enzyme of FAW. Through a combination of steps including centrifugation, ammonium sulfate gradient precipitation, DEAE-Cellulose ion exchange chromatography, the enzyme was purified from fifth instar larvae of FAW. The final purified carboxylesterase after ion exchange chromatography had a specific activity of 7282.22 units / mg protein, 5.6 - fold of crude homogenate, and a yield of 25%. The purity of esterase was established by PAGE and SDS-PAGE. The SDS-PAGE revealed a molecular weight of approximately 45kDa to 66kDa. Our studies on the purified midgut carboxylesterase showed complete inhibition by organophosphorous inhibitor (10-4 M). The enzyme was also inhibited by 1x concentration of Lizol (disinfectant) and by different natural extracts (1x) as well.
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Seed is a developmental stage that is highly protective against external stresses in the plant life cycle. Present study was undertaken with the aim to elucidate the toxic effect of Cu (II) ions on the antioxidant enzymes in chickpea cultivars during seed development and growth. Seven-day-old seedlings were subjected to different concentrations (0-100 ppm) of CuSO 4*7H 2O for 2 days followed by analysis of effect on different antioxidant enzymes. Our results indicated that with the increase in the Cu ion concentration, the antioxidant activities of catalase, ascorbate peroxidase and superoxide dismutase showed a differential behavioral pattern. Besides the antioxidant activity, the toxic affect was also observed in other physiological parameters viz. root/shoot length, RWC and lipid peroxidation. This study indicates that the toxicity of Cu stress is dependent on the physiological state of tissue surrounding the embryo is in part responsible for determining the toxicity.
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